Kumar A Clerici M Muckenfuss LM Passmore LA Jinek M 2019 The polyadenosine (poly(A)) tail found on the 3'-end of almost all eukaryotic mRNAs is important for mRNA stability and regulation of translation. mRNA 3'-end processing occurs co-transcriptionally and involves more than 20 proteins to specifically recognize the polyadenylation site, cleave the pre-mRNA, add a poly(A) tail, and trigger transcription termination. The polyadenylation site (PAS) defines the end of the 3'-untranslated region (3'-UTR) and, therefore, selection of the cleavage site is a critical event in regulating gene expression. Integrated structural biology approaches including biochemical reconstitution of multi-subunit complexes, cross-linking mass spectrometry, and structural analyses by X- ray crystallography and single-particle electron cryo-microscopy (cryoEM) have enabled recent progress in understanding the molecular mechanisms of the mRNA 3'-end processing machinery. Here, we describe new molecular insights into pre-mRNA recognition, cleavage and polyadenylation. https://sonar.ch/global/documents/258750 eng info:eu-repo/semantics/altIdentifier/doi/10.1016/j.sbi.2019.08.001 info:eu-repo/semantics/altIdentifier/pmid/31499460 Current opinion in structural biology. - 2019 3' Untranslated Regions Binding Sites Eukaryota Models, Molecular Polyadenylation Protein Binding RNA Processing, Post-Transcriptional RNA, Messenger RNA-Binding Proteins Structure-Activity Relationship Mechanistic insights into mRNA 3'-end processing. http://purl.org/coar/resource_type/c_6501