Neef AB Pernot L Schreier VN Scapozza L Luedtke NW 2015 Pathogen-selective labeling was achieved by using the novel gemcitabine metabolite analogue 2'-deoxy-2',2'-difluoro-5-ethynyluridine (dF-EdU) and click chemistry. Cells infected with Herpes Simplex Virus-1 (HSV-1), but not uninfected cells, exhibit nuclear staining upon the addition of dF-EdU and a fluorescent azide. The incorporation of the dF-EdU into DNA depends on its phosphorylation by a herpes virus thymidine kinase (TK). Crystallographic analyses revealed how dF-EdU is well accommodated in the active site of HSV-1 TK, but steric clashes prevent dF-EdU from binding human TK. These results provide the first example of pathogen-enzyme-dependent incorporation and labeling of bioorthogonal functional groups in human cells. application/pdf https://sonar.ch/global/documents/258835 eng info:eu-repo/semantics/altIdentifier/doi/10.1002/anie.201500250 info:eu-repo/semantics/altIdentifier/pmid/25974835 info:eu-repo/semantics/openAccess Angewandte Chemie (International ed. in English). - 2015 bioorthogonal chemistry chemical reporters click chemistry nucleosides viruses Animals Azides Catalytic Domain Chlorocebus aethiops Click Chemistry Fluorescent Dyes Halogenation HeLa Cells Herpes Simplex Herpesvirus 1, Human Humans Microscopy, Fluorescence Models, Molecular Staining and Labeling Thymidine Kinase Uridine Vero Cells A Bioorthogonal Chemical Reporter of Viral Infection. http://purl.org/coar/resource_type/c_6501