Detsch R Rübner M Strissel PL Mohn D Strasser E Stark WJ Strick R Boccaccini AR 2016 BACKGROUND There is limited knowledge regarding differentiation of osteoclasts in the presence of nanoscale bioactive glass (nBG). This investigation examined increasing concentrations of 45S5 nBG and their influence on osteoclast differentiation. MATERIALS & METHODS Different concentrations of 45S5 nBG were cultured up to 14 days with the murine RAW264.7 cell line and human primary monocytes cultured with M-CSF and RANKL. RESULTS Culturing cells for 14 days with 500 μg/ml nBG showed a viability of 100%; however DNA synthesis was reduced, supporting differentiation into osteoclast-like cells. Using RAW cells, activation of nine genes, including cell fusion genes, occurred in an nBG concentration dependent manner. Low concentrations of nBG increased expression of genes involved in commitment to cell fusion, whereas high concentrations increased gene expression supporting osteoclast-like differentiation. CONCLUSION nBG enhances both RAW264.7 and human osteoclast differentiation. nBG controlled gene expression in a concentration dependent manner could reflect normal regulation during bone growth. application/pdf https://sonar.ch/global/documents/265743 eng info:eu-repo/semantics/altIdentifier/doi/10.2217/nnm.16.20 info:eu-repo/semantics/altIdentifier/pmid/27092984 info:eu-repo/semantics/openAccess Nanomedicine (London, England). - 2016 bioactive glass biomaterials bone tissue engineering nanoparticles osteoclasts Animals Biocompatible Materials Cell Differentiation Cell Proliferation Ceramics Gene Expression Regulation, Developmental Glass Humans Mice Monocytes Osteoclasts RAW 264.7 Cells Nanoscale bioactive glass activates osteoclastic differentiation of RAW 264.7 cells. http://purl.org/coar/resource_type/c_6501