<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:creator>Schäfer RJB</dc:creator>
  <dc:creator>Monaco MR</dc:creator>
  <dc:creator>Li M</dc:creator>
  <dc:creator>Tirla A</dc:creator>
  <dc:creator>Rivera-Fuentes P</dc:creator>
  <dc:creator>Wennemers H</dc:creator>
  <dc:date>2019</dc:date>
  <dc:description xmlns:ns0="xml" ns0:lang="en">Bioorthogonal reactions are valuable tools for the selective labeling and imaging of natural products and proteins. Here, we present the reaction between isonitriles and chlorooximes as a ligation that proceeds quickly (k ≈ 1 M-1 s-1) and with high chemoselectivity in an aqueous environment. Imaging of metabolically labeled cell surface glycans underlined the tolerance of the ligation to common functional groups in cellular systems. Live-cell dual-labeling experiments revealed that the isonitrile-chlorooxime ligation is orthogonal to the strain-promoted azide-alkyne cycloaddition.</dc:description>
  <dc:identifier>https://sonar.ch/global/documents/275226</dc:identifier>
  <dc:language>eng</dc:language>
  <dc:relation>info:eu-repo/semantics/altIdentifier/doi/10.1021/jacs.9b07632</dc:relation>
  <dc:relation>info:eu-repo/semantics/altIdentifier/pmid/31710811</dc:relation>
  <dc:source>Journal of the American Chemical Society. - 2019</dc:source>
  <dc:subject xmlns:ns1="xml" ns1:lang="en">Animals</dc:subject>
  <dc:subject xmlns:ns2="xml" ns2:lang="en">CHO Cells</dc:subject>
  <dc:subject xmlns:ns3="xml" ns3:lang="en">Cricetulus</dc:subject>
  <dc:subject xmlns:ns4="xml" ns4:lang="en">Kinetics</dc:subject>
  <dc:subject xmlns:ns5="xml" ns5:lang="en">Nitriles</dc:subject>
  <dc:subject xmlns:ns6="xml" ns6:lang="en">Oximes</dc:subject>
  <dc:subject xmlns:ns7="xml" ns7:lang="en">Polysaccharides</dc:subject>
  <dc:title xmlns:ns8="xml" ns8:lang="en">The Bioorthogonal Isonitrile-Chlorooxime Ligation.</dc:title>
  <dc:type>http://purl.org/coar/resource_type/c_6501</dc:type>
</oai_dc:dc>