Templin C Volkmann J Emmert MY Mocharla P Müller M Kraenkel N Ghadri JR Meyer M Styp-Rekowska B Briand S Klingenberg R Jaguszewski M Matter CM Djonov V Mach F Windecker S Hoerstrup SP Thum T Lüscher TF Landmesser U 2017 OBJECTIVE Proangiogenic effects of mobilized bone marrow-derived stem/progenitor cells are essential for cardiac repair after myocardial infarction. MicroRNAs (miRNA/miR) are key regulators of angiogenesis. We investigated the differential regulation of angio-miRs, that is, miRNAs regulating neovascularization, in mobilized CD34+ progenitor cells obtained from patients with an acute ST-segment-elevation myocardial infarction (STEMI) as compared with those with stable coronary artery disease or healthy subjects. APPROACH AND RESULTS CD34+ progenitor cells were isolated from patients with STEMI (on day 0 and day 5), stable coronary artery disease, and healthy subjects (n=27). CD34+ progenitor cells of patients with STEMI exhibited increased proangiogenic activity as compared with CD34+ cells from the other groups. Using a polymerase chain reaction-based miRNA-array and real-time polymerase chain reaction validation, we identified a profound upregulation of 2 known angio-miRs, that are, miR-378 and let-7b, in CD34+ cells of patients with STEMI. Especially, we demonstrate that miR-378 is a critical regulator of the proangiogenic capacity of CD34+ progenitor cells and its stimulatory effects on endothelial cells in vitro and in vivo, whereas let-7b upregulation in CD34+ cells failed to proof its effect on endothelial cells in vivo. CONCLUSIONS The present study demonstrates a significant upregulation of the angio-miRs miR-378 and let-7b in mobilized CD34+ progenitor cells of patients with STEMI. The increased proangiogenic activity of these cells in patients with STEMI and the observation that in particular miR-378 regulates the angiogenic capacity of CD34+ progenitor cells in vivo suggest that this unique miRNA expression pattern represents a novel endogenous repair mechanism activated in acute myocardial infarction. application/pdf https://sonar.ch/global/documents/278669 eng info:eu-repo/semantics/altIdentifier/doi/10.1161/ATVBAHA.116.308695 info:eu-repo/semantics/altIdentifier/pmid/28062497 info:eu-repo/semantics/openAccess Arteriosclerosis, thrombosis, and vascular biology. - 2017 coronary artery disease endothelial cells microRNAs myocardial infarction real-time polymerase chain reaction Aged Animals Antigens, CD34 Case-Control Studies Cell Movement Cell Proliferation Cells, Cultured Chick Embryo Coculture Techniques Endothelial Progenitor Cells Female Human Umbilical Vein Endothelial Cells Humans Male Mice, Nude MicroRNAs Middle Aged Neovascularization, Physiologic Paracrine Communication ST Elevation Myocardial Infarction Signal Transduction Time Factors Transfection Up-Regulation Increased Proangiogenic Activity of Mobilized CD34+ Progenitor Cells of Patients With Acute ST-Segment-Elevation Myocardial Infarction: Role of Differential MicroRNA-378 Expression. http://purl.org/coar/resource_type/c_6501