Agarwal HK Janicek R Chi SH Perry JW Niggli E Ellis-Davies GC 2016 We have designed a nitroaromatic photochemical protecting group that absorbs visible light in the violet-blue range. The chromophore is a dinitro derivative of bisstyrylthiophene (or BIST) that absorbs light very effectively (ε440 = 66,000 M(-1) cm(-1) and two-photon cross section of 350 GM at 775 nm). We developed a "caged calcium" molecule by conjugation of BIST to a Ca(2+) chelator that upon laser flash photolysis rapidly releases Ca(2+) in <0.2 ms. Using the patch-clamp method the optical probe, loaded with Ca(2+), was delivered into acutely isolated mouse cardiac myocytes, where either one- and two-photon uncaging of Ca(2+) induced highly local or cell-wide physiological Ca(2+) signaling events. application/pdf https://sonar.ch/global/documents/285748 eng info:eu-repo/semantics/altIdentifier/doi/10.1021/jacs.5b11606 info:eu-repo/semantics/altIdentifier/pmid/26974387 info:eu-repo/semantics/openAccess Journal of the American Chemical Society. - 2016 Calcium Compounds Chelating Agents Egtazic Acid Light Photochemical Processes Thiophenes Calcium Uncaging with Visible Light. http://purl.org/coar/resource_type/c_6501