Orfanidou CG Beta C Reynard JS Tsiolakis G Katis NI Maliogka VI 2020 A new cytorhabdovirus was identified in zucchini (Cucurbita pepo) in Greece with the aid of high-throughput sequencing technology. The negative-sense, single-stranded genomic RNA of the new virus was determined and includes seven open reading frames in the order 3'-N-P-P3-P4-M-G-L-5' in the antigenomic orientation. Typical rhabdovirus-like particles were observed in infected leaf material. Comparative sequence analysis and phylogenetic reconstructions suggested that the described virus is a new member of the genus Cytorhabdovirus, and it was tentatively named cucurbit cytorhabdovirus 1 (CuCV1). To our knowledge CuCV1 is the first cytorhabdovirus infecting cucurbits in nature. Our surveys indicated that it occurs in a percentage of 36.7 % in zucchini crops in Greece. https://sonar.ch/global/documents/292245 eng info:eu-repo/semantics/altIdentifier/doi/10.1016/j.virusres.2020.198095 info:eu-repo/semantics/altIdentifier/pmid/32735997 Virus research. - 2020 Cucurbits Cytorhabdovirus Detection Electron microscopy HTS Phylogeny Identification, molecular characterization and prevalence of a novel cytorhabdovirus infecting zucchini crops in Greece. http://purl.org/coar/resource_type/c_6501