Lenssen, Eve Azzouz, Nowel Michel, Agnès Landrieux, Emilie Collart, Martine A. <jats:title>ABSTRACT</jats:title> <jats:p>The Ccr4-Not complex is a multifunctional regulatory platform composed of nine subunits that controls diverse cellular events including mRNA degradation, protein ubiquitination, and transcription. In this study, we identified the yeast <jats:italic>Saccharomyces cerevisiae</jats:italic> osmotic and oxidative stress transcription factor Skn7 as a new target for regulation by the Ccr4-Not complex. Skn7 interacts with Not1 in a two-hybrid assay and coimmunoprecipitates with Not5 in a Not4-dependent manner. Skn7-dependent expression of <jats:italic>OCH1</jats:italic> and Skn7 binding to the <jats:italic>OCH1</jats:italic> promoter are increased in <jats:italic>not4</jats:italic>Δ or <jats:italic>not5</jats:italic>Δ mutants. Skn7 purified from wild-type cells but not from <jats:italic>not4</jats:italic>Δ cells is associated with the Srb10 kinase. This kinase plays a central role in the regulation of Skn7 by Not4, since increased <jats:italic>OCH1</jats:italic> expression in <jats:italic>not4</jats:italic>Δ cells requires Srb10. These results reveal a critical role for the Ccr4-Not complex in the mechanism of activation of Skn7 that is dependent upon the Srb10 kinase.</jats:p> application/pdf https://sonar.ch/global/documents/29657 eng info:eu-repo/semantics/altIdentifier/doi/10.1128/ec.00327-06 info:eu-repo/semantics/altIdentifier/issn/1535-9778 info:eu-repo/semantics/openAccess Eukaryotic Cell. - American Society for Microbiology. - 2007, vol. 6, no. 12, p. 2251-2259 Molecular Biology Microbiology General Medicine The Ccr4-Not Complex Regulates Skn7 through Srb10 Kinase http://purl.org/coar/resource_type/c_6501