Hofmann M Wyler R 1988 Porcine epidemic diarrhea virus (PEDV) was adapted to serial propagation in Vero cell cultures by adding trypsin to the medium. PEDV-infected cells showed a distinct cytoplasmic fluorescence when examined by a fluorescent-antibody-staining technique. Cytopathic effects, such as vacuolation, formation of syncytia, and fusion of cells, were detected even at passage 1 of the PEDV in Vero cells. Once adapted, the virus induced numerous syncytia containing over 100 nuclei. From virus passage 5 on, all cells forming the monolayer were fused and totally destroyed within 24 h after inoculation. Cell culture-grown PEDV had typical coronavirus morphology when viewed by electron microscopy. Attempts to propagate PEDV in several primary and secondary fetal porcine cell cultures in the presence or absence of trypsin were unsuccessful. application/pdf https://sonar.ch/global/documents/30383 eng info:eu-repo/semantics/altIdentifier/doi/10.1128/JCM.26.11.2235-2239.1988 info:eu-repo/semantics/altIdentifier/pmid/2853174 info:eu-repo/semantics/openAccess Journal of clinical microbiology. - 1988 Animals Coronaviridae Culture Techniques Fluorescent Antibody Technique Microscopy, Electron Transmissible gastroenteritis virus Vero Cells Viral Plaque Assay Propagation of the virus of porcine epidemic diarrhea in cell culture. http://purl.org/coar/resource_type/c_6501