Nordmann, Patrice Kerbol, Auriane Bouvier, Maxime Sadek, Mustafa Poirel, Laurent Hallal Ferreira Raro, Otavio 2023 Background: The treatment options for infections caused by carbapenem-resistant Enterobacterales (CRE) are extremely scarce nowadays and the development of new antibiotics does not follow the exponential increase in the dissemination of carbapenem resistance determinants worldwide. Meropenem/vaborbactam was recently approved for clinical use and it has been indicated for treating several infections. Although relatively rare, meropenem/vaborbactam resistance has already been reported in Enterobacterales and its early detection could be a valuable tool for faster clinical decision-making. Objectives: To develop a rapid test, namely the Rapid MEV NP, for the identification of meropenem/vaborbactam resistance in Enterobacterales. Methods: The Rapid MEV NP test is based on detection of glucose metabolization occurring upon bacterial growth in the presence of meropenem/vaborbactam at a concentration of 16/8 mg/L. Bacterial growth is detectable by a colour change of phenol red (from red to yellow) subsequent of the acidification of the medium upon bacterial growth. A total of 75 Enterobacterales isolates were randomly selected for evaluating the performance of the Rapid MEV NP test. Results: The test showed 97.2% sensitivity and 93.8% specificity when compared with the reference method. The results are obtained after 3 h of incubation at 35°C ± 2°C, which is a gain of time of at least 15 h (one day in practice) compared with currently used antimicrobial susceptibility testing including broth microdilution methods. Conclusions: The Rapid MEV NP test, easy to perform and to interpret, showed remarkable performance while providing fast results, and is therefore suitable for implementation in routine clinical microbiology laboratories. application/pdf https://sonar.ch/global/documents/328449 https://sonar.ch/documents/328449/files/nordmann2023_mev.pdf eng info:eu-repo/semantics/altIdentifier/doi/10.1093/jac/dkad224 info:eu-repo/semantics/altIdentifier/issn/0305-7453 info:eu-repo/semantics/altIdentifier/issn/1460-2091 info:eu-repo/semantics/openAccess CC BY Journal of Antimicrobial Chemotherapy. - Oxford, UK : Oxford University Press. - 2023, vol. 78, no. 10, p. 1-7 color infections meropenem antimicrobial susceptibility test vaborbactam info:eu-repo/classification/udc/61 Rapid meropenem/vaborbactam NP test for detecting susceptibility/resistance in Enterobacterales http://purl.org/coar/resource_type/c_6501