Meunier D Findlay J Doumith M Godoy D Perry C Pike R Gronthoud F Shryane T Poirel L Welfare W Woodford N Hopkins KL 2017 Objectives Detection of rarer carbapenemases is challenging, as it requires molecular assays with comprehensive coverage or the use of phenotypic methods for the detection of carbapenemase activity. We describe a new class A carbapenemase, FRI-2, in an Enterobacter cloacae complex isolate following implementation of an in-house multiplex PCR for the detection of 'rare' class A carbapenemases. Methods MICs were determined by agar dilution. A carbapenem-resistant E. cloacae complex isolate was tested by PCR for the class A carbapenemases blaKPC, blaFRI, blaIMI, blaGES and blaSME. Carbapenemase activity was assessed using Carba NP and the carbapenem inactivation method. Whole genome and plasmid analyses of the clinical isolate and the FRI-2 transformant were performed by WGS, respectively. Typing was carried out by PFGE. Results The E. cloacae complex isolate showed resistance to imipenem (MIC = 16 mg/L), meropenem (MIC = 8 mg/L) and ertapenem (MIC = 8 mg/L), but remained susceptible to piperacillin/tazobactam (MIC = 8 mg/L). Carbapenemase activity was confirmed in the isolate by both phenotypic methods. A blaFRI-1-like gene was detected by PCR and analysis of WGS data of the clinical isolate identified an ORF of 885 bp, which showed 97% nucleotide identity with blaFRI-1 and was named blaFRI-2. WGS of the transformant indicated blaFRI-2 was located on a 108 kb IncF/IncR plasmid. The FRI-2-positive E. cloacae complex isolate belonged to a novel ST (ST829). Conclusions The possible circulation of rarer carbapenemases in clinical settings highlights the role of phenotypic tests to detect carbapenemase activity when molecular assays are negative for the 'big 5' carbapenemase families. application/pdf https://sonar.ch/global/documents/35245 eng info:eu-repo/semantics/altIdentifier/doi/10.1093/jac/dkx173 info:eu-repo/semantics/altIdentifier/pmid/28605515 info:eu-repo/semantics/openAccess The Journal of antimicrobial chemotherapy. - 2017 Anti-Bacterial Agents Bacterial Proteins Carbapenems Drug Resistance, Multiple, Bacterial Enterobacter cloacae Enterobacteriaceae Infections Genome, Bacterial High-Throughput Nucleotide Sequencing Humans Microbial Sensitivity Tests Multiplex Polymerase Chain Reaction Plasmids United Kingdom beta-Lactamases FRI-2 carbapenemase-producing Enterobacter cloacae complex in the UK. http://purl.org/coar/resource_type/c_6501