Neef AB Pernot L Schreier VN Scapozza L Luedtke NW 2015 Pathogen-selective labeling was achieved by using the novel gemcitabine metabolite analogue 2'-deoxy-2',2'-difluoro-5-ethynyluridine (dF-EdU) and click chemistry. Cells infected with Herpes Simplex Virus-1 (HSV-1), but not uninfected cells, exhibit nuclear staining upon the addition of dF-EdU and a fluorescent azide. The incorporation of the dF-EdU into DNA depends on its phosphorylation by a herpes virus thymidine kinase (TK). Crystallographic analyses revealed how dF-EdU is well accommodated in the active site of HSV-1 TK, but steric clashes prevent dF-EdU from binding human TK. These results provide the first example of pathogen-enzyme-dependent incorporation and labeling of bioorthogonal functional groups in human cells. application/pdf https://sonar.ch/global/documents/3725 eng info:eu-repo/semantics/altIdentifier/doi/10.1002/ange.201500250 info:eu-repo/semantics/altIdentifier/pmid/32313318 info:eu-repo/semantics/openAccess Angewandte Chemie (Weinheim an der Bergstrasse, Germany). - 2015 Bioorthogonale Chemie Chemische Reporter Klick‐Chemie Nukleoside Viren A Bioorthogonal Chemical Reporter of Viral Infection. http://purl.org/coar/resource_type/c_6501