Bulat N Widmann C 2008 Ability to induce protein expression at will in a cell is a powerful strategy used by scientists to better understand the function of a protein of interest. Various inducible systems have been designed in eukaryotic cells to achieve this goal. Most of them rely on two distinct vectors, one encoding a protein that can regulate transcription by binding a compound X, and one hosting the cDNA encoding the protein of interest placed downstream of promoter sequences that can bind the protein regulated by compound X (e.g., tetracycline, ecdysone). The commercially available systems are not designed to allow cell- or tissue-specific regulated expression. Additionally, although these systems can be used to generate stable clones that can be induced to express a given protein, extensive screening is often required to eliminate the clones that display poor induction or high basal levels. In the present report, we aimed to design a pancreatic beta cell-specific tetracycline-inducible system. Since the classical two-vector based tetracycline-inducible system proved to be unsatisfactory in our hands, a single vector was eventually designed that allowed tight beta cell-specific tetracycline induction in unselected cell populations. application/pdf https://sonar.ch/global/documents/46345 eng info:eu-repo/semantics/altIdentifier/doi/10.2144/000112947 info:eu-repo/semantics/altIdentifier/pmid/18855768 info:eu-repo/semantics/openAccess BioTechniques. - 2008 Animals COS Cells Chlorocebus aethiops GTPase-Activating Proteins Gene Expression Regulation Genetic Techniques Genetic Vectors Humans Insulin-Secreting Cells Mice Organ Specificity Plasmids Response Elements Tetracycline Generation of a tightly regulated all-cis beta cell-specific tetracycline-inducible vector. http://purl.org/coar/resource_type/c_6501