Peloso A Citro A Corradetti V Brambilla S Oldani G Calabrese F Dominioni T Maestri M Cobianchi L 2018 Whole-organ decellularization is recently gaining interest in the transplantation field as strategy to obtain acellular scaffold only composed by extracellular matrix. These structures, that still remain organ-specific in terms of biological cues and tridimensional morphology could be then recellularized with patient's autologous cells. The final result should be a nwe transplantable autologous organ that should by-pass, at the same time, the problem of organ shortage and secondly the consequences related to the immunosuppression need. Herein we describe the protocol to manufacture a whole-organ transplantable rat kidney scaffold by a dual-detergent (Triton X-100 and SDS) arterial peristaltic perfusion. Final results show whole-renal acellular scaffold with contextual preservation of tridimensional architecture and biological properties deriving from the extracellular matrix composition. https://sonar.ch/global/documents/46491 eng info:eu-repo/semantics/altIdentifier/doi/10.1007/7651_2017_96 info:eu-repo/semantics/altIdentifier/pmid/29159728 Methods in molecular biology (Clifton, N.J.). - 2018 Extracellular matrix Kidney scaffold Kidney transplantation Organ bioengineering Rat model Regenerative medicine Animals Detergents Extracellular Matrix Kidney Perfusion Rats Tissue Engineering Tissue Scaffolds In-Lab Manufacturing of Decellularized Rat Renal Scaffold for Kidney Bioengineering. http://purl.org/coar/resource_type/c_6501