Kovac M Risch L Thiel S Weber M Grossmann K Wohlwend N Lung T Hillmann D Ritzler M Bigler S Ferrara F Bodmer T Egli K Imperiali M Heer S Salimi Y Renz H Kohler P Vernazza P Kahlert CR Paprotny M Risch M 2020 While lateral flow test formats can be utilized with whole blood and low sample volumes, their diagnostic characteristics are inferior to immunoassays based on chemiluminescence immunoassay (CLIA) or enzyme-linked immunosorbent assay (ELISA) technology. CLIAs and ELISAs can be automated to a high degree but commonly require larger serum or plasma volumes for sample processing. We addressed the suitability of EDTA-anticoagulated whole blood as an alternative sample material for antibody testing against SARS-CoV-2 by electro-CLIA (ECLIA; Roche, Rotkreuz, Switzerland) and ELISA (IgG and IgA; Euroimmun, Germany). Simultaneously drawn venous serum and EDTA-anticoagulated whole blood samples from 223 individuals were included. Correction of the whole blood results for hematocrit led to a good agreement with the serum results for weakly to moderately positive antibody signals. In receiver-operating characteristic curve analysis, all three assays displayed comparable diagnostic accuracy (area under the curve (AUC)) using corrected whole blood and serum (AUCs: 0.97 for ECLIA and IgG ELISA; 0.84 for IgA ELISA). In conclusion, our results suggest that the investigated assays can reliably detect antibodies against SARS-CoV-2 in hemolyzed whole blood anticoagulated with EDTA. Correction of these results for hematocrit is suggested. This study demonstrates that the automated processing of whole blood for identification of SARS-CoV-2 antibodies with common ECLIA and ELISA methods is accurate and feasible. application/pdf https://sonar.ch/global/documents/46847 eng info:eu-repo/semantics/altIdentifier/doi/10.3390/diagnostics10080593 info:eu-repo/semantics/altIdentifier/pmid/32823852 info:eu-repo/semantics/openAccess Diagnostics (Basel, Switzerland). - 2020 COVID-19 SARS-CoV-2 antibodies preanalytics sensitivity serum specificity whole blood EDTA-Anticoagulated Whole Blood for SARS-CoV-2 Antibody Testing by Electrochemiluminescence Immunoassay (ECLIA) and Enzyme-Linked Immunosorbent Assay (ELISA). http://purl.org/coar/resource_type/c_6501