Ganter B Tan S Richmond TJ 1993 Dimethyl sulfate, DNase I and micrococcal nuclease DNA cleavage were combined with the ligation-mediated polymerase chain reaction to obtain high resolution maps of the promoter regions for two cell-type-specific genes: the a-specific STE2 gene and the alpha-specific STE3 gene. We find that MCM1 binds in vivo in a-cells to a 16 bp P-box sequence located in the STE2 UAS. In alpha-cells, the footprint pattern is extended relative to a-cells, consistent with the additional binding of MAT alpha 2 to the sequences flanking each end of the P-box. A nucleosome was found adjacent to the P-box of the transcriptionally repressed a-specific STE2 UAS in alpha-cells, positioned so that the nucleosome overlaps the TATA-box. In contrast, such well-positioned nucleosomes were not found for the transcriptionally active STE2 UAS in a-cells, where instead the TATA box appears to be bound to the general transcription factor TFIID. These observations support the hypothesis that MAT alpha 2 repression of a-specific genes is mediated by nucleosomes, perhaps by exclusion of TFIID from the TATA-box. https://sonar.ch/global/documents/47406 eng info:eu-repo/semantics/altIdentifier/doi/10.1006/jmbi.1993.1652 info:eu-repo/semantics/altIdentifier/pmid/8263944 Journal of molecular biology. - 1993 Base Sequence DNA Primers Fungal Proteins Gene Expression Regulation, Fungal Genes, Fungal Molecular Sequence Data Nucleosomes Promoter Regions, Genetic RNA, Messenger Receptors, Cell Surface Receptors, G-Protein-Coupled Receptors, Mating Factor Receptors, Peptide Receptors, Pheromone Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins TATA Box Transcription Factors Genomic footprinting of the promoter regions of STE2 and STE3 genes in the yeast Saccharomyces cerevisiae. http://purl.org/coar/resource_type/c_6501