Fournier MF Sauser R Ambrosi D Meister JJ Verkhovsky AB 2010 During cell migration, forces generated by the actin cytoskeleton are transmitted through adhesion complexes to the substrate. To investigate the mechanism of force generation and transmission, we analyzed the relationship between actin network velocity and traction forces at the substrate in a model system of persistently migrating fish epidermal keratocytes. Front and lateral sides of the cell exhibited much stronger coupling between actin motion and traction forces than the trailing cell body. Further analysis of the traction-velocity relationship suggested that the force transmission mechanisms were different in different cell regions: at the front, traction was generated by a gripping of the actin network to the substrate, whereas at the sides and back, it was produced by the network's slipping over the substrate. Treatment with inhibitors of the actin-myosin system demonstrated that the cell body translocation could be powered by either of the two different processes, actomyosin contraction or actin assembly, with the former associated with significantly larger traction forces than the latter. application/pdf https://sonar.ch/global/documents/48596 eng info:eu-repo/semantics/altIdentifier/doi/10.1083/jcb.200906139 info:eu-repo/semantics/altIdentifier/pmid/20100912 info:eu-repo/semantics/openAccess The Journal of cell biology. - 2010 Actin Cytoskeleton Animals Biomechanical Phenomena Cell Adhesion Cell Movement Cell Polarity Cell Shape Cells, Cultured Cytochalasin D Cytoskeleton Fishes Heterocyclic Compounds, 4 or More Rings Keratinocytes Models, Biological Myosins Protein Synthesis Inhibitors Stress, Mechanical Tensile Strength Force transmission in migrating cells. http://purl.org/coar/resource_type/c_6501