<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:creator>Lanz C</dc:creator>
  <dc:creator>Ledermann M</dc:creator>
  <dc:creator>Slavík J</dc:creator>
  <dc:creator>Idle JR</dc:creator>
  <dc:date>2011</dc:date>
  <dc:description xmlns:ns0="xml" ns0:lang="en">PURPOSE
The aim of this work was to use metabolomics to evaluate sebum as a source of biomarkers for gamma-radiation exposure in the rat, and potentially in man. Proof of concept of radiation metabolomics was previously demonstrated in both mouse and rat urine, from the radiation dose- and time-dependent excretion of a set of urinary biomarkers.


MATERIALS AND METHODS
Rats were gamma-irradiated (3 Gy) or sham irradiated and groups of rats were euthanised at 1 h or 24 h post-irradiation. Sebum was collected by multiple washings of the carcasses with acetone. Nonpolar lipids were extracted, methylated, separated and quantitated using gas chromatography-mass spectrometry (GCMS). Metabolomic analysis of the GCMS data was performed using both orthogonal projection to latent structures-discriminant analysis and random forests machine learning algorithm.


RESULTS
Irradiation did not alter sebum production. A total of 35 lipids were identified in rat sebum, 29 fatty acids, five fatty aldehydes, and cholesterol. Metabolomics showed that three fatty acids, palmitic, 2-hydroxypalmitic, and stearic acids were potential biomarkers. Sebaceous palmitic acid was marginally statistically significantly elevated (7.5-8.4%) at 24 h post-irradiation.


CONCLUSIONS
Rat sebaceous gland appears refractory to 3 Gy gamma-irradiation. Unfortunately, collection of sebum shortly after gamma-irradiation is unlikely to form the basis of high-throughput non-invasive radiation biodosimetry in man.</dc:description>
  <dc:format>application/pdf</dc:format>
  <dc:identifier>https://sonar.ch/global/documents/59102</dc:identifier>
  <dc:language>eng</dc:language>
  <dc:relation>info:eu-repo/semantics/altIdentifier/doi/10.3109/09553002.2010.537432</dc:relation>
  <dc:relation>info:eu-repo/semantics/altIdentifier/pmid/21158499</dc:relation>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:source>International journal of radiation biology. - 2011</dc:source>
  <dc:subject xmlns:ns1="xml" ns1:lang="en">Animals</dc:subject>
  <dc:subject xmlns:ns2="xml" ns2:lang="en">Biological Assay</dc:subject>
  <dc:subject xmlns:ns3="xml" ns3:lang="en">Biomarkers</dc:subject>
  <dc:subject xmlns:ns4="xml" ns4:lang="en">Body Burden</dc:subject>
  <dc:subject xmlns:ns5="xml" ns5:lang="en">Gamma Rays</dc:subject>
  <dc:subject xmlns:ns6="xml" ns6:lang="en">Male</dc:subject>
  <dc:subject xmlns:ns7="xml" ns7:lang="en">Mice</dc:subject>
  <dc:subject xmlns:ns8="xml" ns8:lang="en">Radiation Dosage</dc:subject>
  <dc:subject xmlns:ns9="xml" ns9:lang="en">Rats</dc:subject>
  <dc:subject xmlns:ns10="xml" ns10:lang="en">Rats, Inbred F344</dc:subject>
  <dc:subject xmlns:ns11="xml" ns11:lang="en">Sebaceous Glands</dc:subject>
  <dc:subject xmlns:ns12="xml" ns12:lang="en">Sebum</dc:subject>
  <dc:subject xmlns:ns13="xml" ns13:lang="en">Whole-Body Counting</dc:subject>
  <dc:subject xmlns:ns14="xml" ns14:lang="en">Whole-Body Irradiation</dc:subject>
  <dc:title xmlns:ns15="xml" ns15:lang="en">The production and composition of rat sebum is unaffected by 3 Gy gamma radiation.</dc:title>
  <dc:type>http://purl.org/coar/resource_type/c_6501</dc:type>
</oai_dc:dc>