<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:creator>Müller KM</dc:creator>
  <dc:creator>Bickel M</dc:creator>
  <dc:creator>Wiesmann UN</dc:creator>
  <dc:creator>Spörri B</dc:creator>
  <dc:date>2000</dc:date>
  <dc:description xmlns:ns0="xml" ns0:lang="en">Human dermal fibroblasts (HDF) undergo activation and secrete cytokines when cocultured with T cells. Here, we identify potent activators of HDF among human peripheral CD2(+)-lymphocytes. Populations with strong HDF activating capacity consisted essentially of cells with a natural killer (NK) surface marker phenotype (CD3(-), CD4(-), CD8(-), CD56(+)). Addition of these cells to HDF resulted in rapid increase of intracellular free calcium concentrations as an early rapid cell activation signal. Upregulation of mRNA encoding for the inflammatory cytokines IL-1 beta and IL-6 as well as for chemokines IL-8 and MCP-1 was detected after cells were cocultured. Elevated concentrations of IL-6 and IL-8 were found in coculture supernatants of HDF and NK-cells. Skin-homing NK cells leaving the blood-stream during an inflammatory skin reaction might therefore represent potent activators of local inflammatory cytokine and chemokine production.</dc:description>
  <dc:identifier>https://sonar.ch/global/documents/63936</dc:identifier>
  <dc:language>eng</dc:language>
  <dc:relation>info:eu-repo/semantics/altIdentifier/doi/10.1006/cyto.2000.0787</dc:relation>
  <dc:relation>info:eu-repo/semantics/altIdentifier/pmid/11097744</dc:relation>
  <dc:source>Cytokine. - 2000</dc:source>
  <dc:subject xmlns:ns1="xml" ns1:lang="en">CD2 Antigens</dc:subject>
  <dc:subject xmlns:ns2="xml" ns2:lang="en">CD3 Complex</dc:subject>
  <dc:subject xmlns:ns3="xml" ns3:lang="en">CD4 Antigens</dc:subject>
  <dc:subject xmlns:ns4="xml" ns4:lang="en">CD56 Antigen</dc:subject>
  <dc:subject xmlns:ns5="xml" ns5:lang="en">CD8 Antigens</dc:subject>
  <dc:subject xmlns:ns6="xml" ns6:lang="en">Calcium</dc:subject>
  <dc:subject xmlns:ns7="xml" ns7:lang="en">Cell Count</dc:subject>
  <dc:subject xmlns:ns8="xml" ns8:lang="en">Cell Division</dc:subject>
  <dc:subject xmlns:ns9="xml" ns9:lang="en">Cell Membrane</dc:subject>
  <dc:subject xmlns:ns10="xml" ns10:lang="en">Chemokine CCL2</dc:subject>
  <dc:subject xmlns:ns11="xml" ns11:lang="en">Coculture Techniques</dc:subject>
  <dc:subject xmlns:ns12="xml" ns12:lang="en">Dose-Response Relationship, Drug</dc:subject>
  <dc:subject xmlns:ns13="xml" ns13:lang="en">Enzyme-Linked Immunosorbent Assay</dc:subject>
  <dc:subject xmlns:ns14="xml" ns14:lang="en">Fibroblasts</dc:subject>
  <dc:subject xmlns:ns15="xml" ns15:lang="en">Flow Cytometry</dc:subject>
  <dc:subject xmlns:ns16="xml" ns16:lang="en">Humans</dc:subject>
  <dc:subject xmlns:ns17="xml" ns17:lang="en">Inflammation</dc:subject>
  <dc:subject xmlns:ns18="xml" ns18:lang="en">Interleukin-1</dc:subject>
  <dc:subject xmlns:ns19="xml" ns19:lang="en">Interleukin-6</dc:subject>
  <dc:subject xmlns:ns20="xml" ns20:lang="en">Interleukin-8</dc:subject>
  <dc:subject xmlns:ns21="xml" ns21:lang="en">Killer Cells, Natural</dc:subject>
  <dc:subject xmlns:ns22="xml" ns22:lang="en">Leukocytes, Mononuclear</dc:subject>
  <dc:subject xmlns:ns23="xml" ns23:lang="en">Microscopy, Fluorescence</dc:subject>
  <dc:subject xmlns:ns24="xml" ns24:lang="en">Phenotype</dc:subject>
  <dc:subject xmlns:ns25="xml" ns25:lang="en">RNA</dc:subject>
  <dc:subject xmlns:ns26="xml" ns26:lang="en">RNA, Messenger</dc:subject>
  <dc:subject xmlns:ns27="xml" ns27:lang="en">Time Factors</dc:subject>
  <dc:subject xmlns:ns28="xml" ns28:lang="en">Up-Regulation</dc:subject>
  <dc:title xmlns:ns29="xml" ns29:lang="en">Natural killer cells activate human dermal fibroblasts.</dc:title>
  <dc:type>http://purl.org/coar/resource_type/c_6501</dc:type>
</oai_dc:dc>