Cardo L Karunatilaka KS Rueda D Sigel RK 2012 A procedure to investigate the folding of group II intron by single molecule Fluorescence Resonance Energy Transfer (smFRET) using total internal reflection fluorescence microscopy (TIRFM) is described in this chapter. Using our previous studies on the folding and dynamics of a large ribozyme in the presence of metal ions (i.e., Mg(2+) and Ca(2+)) and/or the DEAD-box protein Mss116 as an example, we here describe step-by-step procedures to perform experiments. smFRET allows the investigation of individual molecules, thus, providing kinetic and mechanistic information hidden in ensemble averaged experiments. application/pdf https://sonar.ch/global/documents/65865 eng info:eu-repo/semantics/altIdentifier/doi/10.1007/978-1-61779-545-9_15 info:eu-repo/semantics/altIdentifier/pmid/22315073 info:eu-repo/semantics/openAccess Methods in molecular biology (Clifton, N.J.). - 2012 Base Sequence Calibration DEAD-box RNA Helicases Fluorescence Polarization Fluorescence Resonance Energy Transfer Introns Magnesium Chloride Microfluidic Analytical Techniques Molecular Sequence Data Nucleic Acid Conformation Polyethylene Glycols RNA, Catalytic Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Transcription, Genetic Single molecule FRET characterization of large ribozyme folding. http://purl.org/coar/resource_type/c_6501