Lettieri T Kraehenbuehl R Capiaghi C Livingstone-Zatchej M Thoma F 2008 In yeast, Rad7 and Rad16 are two proteins required for nucleotide excision repair (NER) of non-transcribed chromatin. They have roles in damage recognition, in the postincision steps of NER, and in ultraviolet-light-dependent histone H3 acetylation. Moreover, Rad16 is an ATP-ase of the SNF2 superfamily and therefore might facilitate chromatin repair by nucleosome remodelling. Here, we used yeast rad7 Delta rad16 Delta mutants and show that Rad7-Rad16 is also required for NER of UV-lesions in three functionally distinct nucleosome-free regions (NFRs), the promoter and 3'-end of the URA3 gene and the ARS1 origin of replication. Moreover, rapid repair of UV-lesions by photolyase confirmed that nucleosomes were absent and that neither UV-damage formation nor rad7 Delta rad16 Delta mutations altered chromatin accessibility in NFRs. The data are consistent with a role of Rad7-Rad16 in damage recognition and processing in absence of nucleosomes. An additional role in nucleosome remodelling is discussed. https://sonar.ch/global/documents/67195 eng info:eu-repo/semantics/altIdentifier/doi/10.1016/j.dnarep.2008.01.016 info:eu-repo/semantics/altIdentifier/pmid/18329964 DNA repair. - 2008 Adenosine Triphosphatases DNA Repair DNA, Fungal DNA-Binding Proteins Fungal Proteins Mutation Nucleosomes Pyrimidine Dimers Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors Functionally distinct nucleosome-free regions in yeast require Rad7 and Rad16 for nucleotide excision repair. http://purl.org/coar/resource_type/c_6501