Kuratli S Hemphill A Lindh J Smith DF Connolly B 2001 The Trichinella tsJ5 gene is preferentially expressed in muscle larvae of Trichinella spiralis and encodes a novel protein. Previous observations have shown tsJ5 to be expressed at higher levels in encapsulating species than in non-encapsulating species and down-regulation of gene expression in T. pseudospiralis to be correlated with a lower protein abundance in the muscle larva of this species. In the present study we have determined the full-length cDNA sequence of the tsJ5 homologue in T. pseudospiralis (tpJ5). Antigens recognised by an anti-J5 antibody are found on the cuticular surface of both T. spiralis and T. pseudospiralis muscle larvae, as well as in the body wall muscle. We show that both the TSJ5 and TPJ5 proteins are found in the excretory/secretory fractions collected from muscle larva cultured in vitro and that despite the absence of a typical N-terminal signal sequence, secretion of pTSJ5 is mediated through the classical ER/Golgi secretory pathway. https://sonar.ch/global/documents/70757 eng info:eu-repo/semantics/altIdentifier/doi/10.1016/s0166-6851(01)00287-0 info:eu-repo/semantics/altIdentifier/pmid/11420106 Molecular and biochemical parasitology. - 2001 Amino Acid Sequence Animals DNA, Complementary DNA, Helminth Fluorescent Antibody Technique Helminth Proteins Larva Mice Mice, Inbred ICR Molecular Sequence Data Muscle Proteins Muscle, Skeletal Repressor Proteins Sequence Analysis, DNA Trichinella Trichinella spiralis Trichinellosis Secretion of the novel Trichinella protein TSJ5 by T. spiralis and T. pseudospiralis muscle larvae. http://purl.org/coar/resource_type/c_6501