Schermelleh L Ferrand A Huser T Eggeling C Sauer M Biehlmaier O Drummen GPC 2019 Super-resolution microscopy (SRM) bypasses the diffraction limit, a physical barrier that restricts the optical resolution to roughly 250 nm and was previously thought to be impenetrable. SRM techniques allow the visualization of subcellular organization with unprecedented detail, but also confront biologists with the challenge of selecting the best-suited approach for their particular research question. Here, we provide guidance on how to use SRM techniques advantageously for investigating cellular structures and dynamics to promote new discoveries. application/pdf https://sonar.ch/global/documents/73204 eng info:eu-repo/semantics/altIdentifier/doi/10.1038/s41556-018-0251-8 info:eu-repo/semantics/altIdentifier/pmid/30602772 info:eu-repo/semantics/openAccess Nature cell biology. - 2019 Animals Cell Biology Humans Imaging, Three-Dimensional Microscopy, Fluorescence Molecular Biology Reproducibility of Results Super-resolution microscopy demystified. http://purl.org/coar/resource_type/c_6501