Yu Q Duan L Yu L Chen X Si G Ke P Ye Z Mulder J 2018 The influence of nitrogen (N) deposition on forest ecosystems largely depend on the N status. Developing threshold and practical indicators for N saturation in subtropical forests, with extremely high N deposition, would both enhance forest management and the assessments of global N balance and carbon (C) sequestration. Here, we quantified the N mass balance and assessed current N status at a number of subtropical forest sites in South China, using both N content, C/N ratio, and 15N natural abundance (δ15N) as potential indicators of N saturation. Among the studied sites, N deposition ranged from 13.8 to 113 kg N ha-1 yr-1 in throughfall, and was dominated by ammonium (NH4+). The threshold for N leaching in subtropical forest was first found to be 26-36 kg N ha-1 yr-1, which was 160% higher than in temperate forest (based on prescribed minimum). This indicates that critical parameter inputs in global models of the impact of N deposition are in need of revision, based on specific ecosystem characteristics. We found a critical C/N ratio of 20 for the O/A horizon as indicator of N saturation. Foliar N content and δ15N were positively correlated with N deposition and were well suited to indicate regional N status. The δ15N enrichment factor (Ɛfoli/So2, δ15Nfoliage - δ15NSoil2) was between -10‰ and -1‰, and had similar trend to those obtained from other regions with increasing N deposition. These suggest that the enrichment factor could be used to investigate the influence of N deposition in forest ecosystems, regardless of spatial heterogeneity in δ15N of N input, soil N availability and geomorphology. https://sonar.ch/global/documents/75509 eng info:eu-repo/semantics/altIdentifier/doi/10.1016/j.envpol.2018.06.001 info:eu-repo/semantics/altIdentifier/pmid/29902749 Environmental pollution (Barking, Essex : 1987). - 2018 Enrichment factor N deposition N isotope N saturation Subtropical forests Ammonium Compounds China Ecosystem Environmental Monitoring Forests Nitrogen Nitrogen Cycle Soil Trees Threshold and multiple indicators for nitrogen saturation in subtropical forests. http://purl.org/coar/resource_type/c_6501