<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:creator>Jaeger BN</dc:creator>
  <dc:creator>Yángüez E</dc:creator>
  <dc:creator>Gesuita L</dc:creator>
  <dc:creator>Denoth-Lippuner A</dc:creator>
  <dc:creator>Kruse M</dc:creator>
  <dc:creator>Karayannis T</dc:creator>
  <dc:creator>Jessberger S</dc:creator>
  <dc:date>2020</dc:date>
  <dc:description xmlns:ns0="xml" ns0:lang="en">This protocol presents a plate-based workflow to perform RNA sequencing analysis of single cells/nuclei using Smart-seq2. We describe (1) the dissociation procedures for cell/nucleus isolation from the mouse brain and human organoids, (2) the flow sorting of single cells/nuclei into 384-well plates, and (3) the preparation of libraries following miniaturization of the Smart-seq2 protocol using a liquid-handling robot. This pipeline allows for the reliable, high-throughput, and cost-effective preparation of mouse and human samples for full-length deep single-cell/nucleus RNA sequencing. For complete details on the use and execution of this protocol, please refer to Bowers et al. (2020).</dc:description>
  <dc:identifier>https://sonar.ch/global/documents/79457</dc:identifier>
  <dc:language>eng</dc:language>
  <dc:relation>info:eu-repo/semantics/altIdentifier/doi/10.1016/j.xpro.2020.100081</dc:relation>
  <dc:relation>info:eu-repo/semantics/altIdentifier/pmid/33000004</dc:relation>
  <dc:source>STAR protocols. - 2020</dc:source>
  <dc:title xmlns:ns1="xml" ns1:lang="en">Miniaturization of Smart-seq2 for Single-Cell and Single-Nucleus RNA Sequencing.</dc:title>
  <dc:type>http://purl.org/coar/resource_type/c_6501</dc:type>
</oai_dc:dc>