Gabev, Evgeni Tobler, Kurt Abril, Carlos Hilbe, Monika Senn, Claudia Franchini, Marco Campadelli-Fiume, Gabriella Fraefel, Cornel Ackermann, Mathias <jats:title>ABSTRACT</jats:title> <jats:p>Bovine herpesvirus 1 (BoHV-1) and BoHV-5 are closely related pathogens of cattle, but only BoHV-5 is considered a neuropathogen. We engineered intertypic gD exchange mutants with BoHV-1 and BoHV-5 backbones in order to address their <jats:italic>in vitro</jats:italic> and <jats:italic>in vivo</jats:italic> host ranges, with particular interest in invasion of the brain. The new viruses replicated in cell culture with similar dynamics and to titers comparable to those of their wild-type parents. However, gD of BoHV-5 (gD5) was able to interact with a surprisingly broad range of nectins. <jats:italic>In vivo</jats:italic>, gD5 provided a virulent phenotype to BoHV-1 in AR129 mice, featuring a high incidence of neurological symptoms and early onset of disease. However, only virus with the BoHV-5 backbone, independent of the gD type, was detected in the brain by immunohistology. Thus, gD of BoHV-5 confers an extended cellular host range to BoHV-1 and may be considered a virulence factor but does not contribute to the invasion of the brain.</jats:p> application/pdf https://sonar.ch/global/documents/81972 eng info:eu-repo/semantics/altIdentifier/doi/10.1128/jvi.00228-10 info:eu-repo/semantics/altIdentifier/issn/0022-538X info:eu-repo/semantics/openAccess Journal of Virology. - American Society for Microbiology. - 2010, vol. 84, no. 11, p. 5583-5593 Immunology Insect Science Microbiology Virology Glycoprotein D of Bovine Herpesvirus 5 (BoHV-5) Confers an Extended Host Range to BoHV-1 but Does Not Contribute to Invasion of the Brain http://purl.org/coar/resource_type/c_6501