Vogg MC Galliot B Tsiairis CD 2019 The freshwater polyp Hydra provides a potent model system for investigating the conditions that promote wound healing, reactivation of a developmental process and, ultimately, regeneration of an amputated body part. Hydra polyps can also be dissociated to the single cell level and can regenerate a complete body axis from aggregates, behaving as natural organoids. In recent years, the ability to exploit Hydra has been expanded with the advent of new live-imaging approaches, genetic manipulations that include stable transgenesis, gene silencing and genome editing, and the accumulation of high-throughput omics data. In this Primer, we provide an overview of Hydra as a model system for studying regeneration, highlighting recent results that question the classical self-enhancement and long-range inhibition model supposed to drive Hydra regeneration. We underscore the need for integrative explanations incorporating biochemical as well as mechanical signalling. application/pdf https://sonar.ch/global/documents/92713 eng info:eu-repo/semantics/altIdentifier/doi/10.1242/dev.177212 info:eu-repo/semantics/altIdentifier/pmid/31676551 info:eu-repo/semantics/openAccess Development (Cambridge, England). - 2019 Genetic manipulations Hydra model system Organizer centre Organoid Reaggregation Regeneration Animals Gene Editing Gene Expression Regulation, Developmental Gene Silencing Homeostasis Hydra Models, Biological Organoids Phylogeny Regeneration Signal Transduction Stem Cells Transgenes Wnt Proteins beta Catenin Model systems for regeneration: Hydra. http://purl.org/coar/resource_type/c_6501