<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:creator>Kettner A</dc:creator>
  <dc:creator>Hughes GJ</dc:creator>
  <dc:creator>Frutiger S</dc:creator>
  <dc:creator>Astori M</dc:creator>
  <dc:creator>Roggero M</dc:creator>
  <dc:creator>Spertini F</dc:creator>
  <dc:creator>Corradin G</dc:creator>
  <dc:date>2001</dc:date>
  <dc:description xmlns:ns0="xml" ns0:lang="en">BACKGROUND
Characterization of the primary structure of allergens is a prerequisite for the design of new diagnostic and therapeutic tools for allergic diseases.


OBJECTIVE
The purpose of this study was the identification and characterization of a low-molecular-weight, IgE-binding, bee venom (BV) allergen.


METHODS
BV proteins were separated by using size exclusion chromatography and HPLC. IgE antibody binding to purified proteins was analyzed by means of immunoblotting, and T-cell response was analyzed by means of proliferation assay. Amino acid sequence was determined with 2 approaches, namely Edman degradation and carboxy terminal analysis with mass spectrometry.


RESULTS
Api m 6, which migrated as an 8-kd band in SDS-PAGE, was frequently (42%) recognized by IgE from BV-hypersensitive patients. In addition, PBMCs from BV-hypersensitive patients, as well as from a normal control subject, proliferated in response to this allergen. Api m 6 exists as 4 isoforms of 7190, 7400, 7598, and 7808 d, respectively. Amino acid sequences obtained from HPLC-purified preparations revealed that the isoforms were constituted of a common central core of 67 residues, only differing in the amino- and carboxy-terminal ends. Api m 6 showed no significant sequence homology with known proteins.


CONCLUSIONS
We have identified and sequenced a new BV allergen that elicits a strong IgE and T-cell response in a large number of BV-hypersensitive patients. Api m 6 should be considered in the diagnostic and therapeutic approach of BV immunotherapy on the basis of peptides or recombinant proteins.</dc:description>
  <dc:identifier>https://sonar.ch/global/documents/93549</dc:identifier>
  <dc:language>eng</dc:language>
  <dc:relation>info:eu-repo/semantics/altIdentifier/doi/10.1067/mai.2001.113867</dc:relation>
  <dc:relation>info:eu-repo/semantics/altIdentifier/pmid/11344362</dc:relation>
  <dc:source>The Journal of allergy and clinical immunology. - 2001</dc:source>
  <dc:subject xmlns:ns1="xml" ns1:lang="en">Allergens</dc:subject>
  <dc:subject xmlns:ns2="xml" ns2:lang="en">Amino Acid Motifs</dc:subject>
  <dc:subject xmlns:ns3="xml" ns3:lang="en">Amino Acid Sequence</dc:subject>
  <dc:subject xmlns:ns4="xml" ns4:lang="en">Animals</dc:subject>
  <dc:subject xmlns:ns5="xml" ns5:lang="en">Antibody Specificity</dc:subject>
  <dc:subject xmlns:ns6="xml" ns6:lang="en">Antigens, Plant</dc:subject>
  <dc:subject xmlns:ns7="xml" ns7:lang="en">Bee Venoms</dc:subject>
  <dc:subject xmlns:ns8="xml" ns8:lang="en">Chromatography, Gel</dc:subject>
  <dc:subject xmlns:ns9="xml" ns9:lang="en">Chromatography, High Pressure Liquid</dc:subject>
  <dc:subject xmlns:ns10="xml" ns10:lang="en">Humans</dc:subject>
  <dc:subject xmlns:ns11="xml" ns11:lang="en">Hypersensitivity, Immediate</dc:subject>
  <dc:subject xmlns:ns12="xml" ns12:lang="en">Immunoglobulin E</dc:subject>
  <dc:subject xmlns:ns13="xml" ns13:lang="en">Insect Proteins</dc:subject>
  <dc:subject xmlns:ns14="xml" ns14:lang="en">Lymphocyte Activation</dc:subject>
  <dc:subject xmlns:ns15="xml" ns15:lang="en">Molecular Sequence Data</dc:subject>
  <dc:subject xmlns:ns16="xml" ns16:lang="en">Protein Denaturation</dc:subject>
  <dc:subject xmlns:ns17="xml" ns17:lang="en">T-Lymphocytes</dc:subject>
  <dc:title xmlns:ns18="xml" ns18:lang="en">Api m 6: a new bee venom allergen.</dc:title>
  <dc:type>http://purl.org/coar/resource_type/c_6501</dc:type>
</oai_dc:dc>