<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:creator>Zimmermann-Klemd AM</dc:creator>
  <dc:creator>Reinhardt JK</dc:creator>
  <dc:creator>Nilsu T</dc:creator>
  <dc:creator>Morath A</dc:creator>
  <dc:creator>Falanga CM</dc:creator>
  <dc:creator>Schamel WW</dc:creator>
  <dc:creator>Huber R</dc:creator>
  <dc:creator>Hamburger M</dc:creator>
  <dc:creator>Gründemann C</dc:creator>
  <dc:date>2020</dc:date>
  <dc:description xmlns:ns0="xml" ns0:lang="en">Resins from various Boswellia species have a long track record in different cultures as a treatment for inflammatory diseases. This study was designed to provide evidence for the anti-inflammatory capacity and medicinal use of Boswellia carteri (Burseraceae). A dichloromethane (DCM) extract of B. carteri gum resin and isolated compounds thereof were immunologically characterized. Flow cytometric-based analysis was performed to investigate the impact of B. carteri extract on proliferation, viability, and function of anti-CD3 and anti-CD28 activated human primary T cells. The secretion level of IL-2 and IFN-γ was determined by a bead array-based flow cytometric technique. HPLC-based activity profiling of the B. carteri extract identified active compounds. The impact of B. carteri extract and isolated compounds on the IL-2 transcription factor activity was addressed using specially designed Jurkat reporter cells. The extract of B. carteri suppressed the proliferation of human primary T lymphocytes in vitro in a concentration-dependent manner, without inducing cytotoxicity. Thereby, the B. carteri extract further reduced the degranulation capacity and cytokine secretion of stimulated human T cells. Transcription factor analysis showed that the immunosuppressive effects of the extract are based on specific NFAT-conditioned suppression within T cell signaling. Through HPLC-based activity profiling of the extract, 3-O-acetyl-alpha-boswellic acid was identified as the compound responsible for the NFAT-based mechanism. The recent study presents a scientific base for the immunosuppressive effects of B. carteri gum resin extract including a mode-of-action via the NFAT-conditioned suppression of T lymphocyte proliferation. The immunosuppressive effects of 3-O-acetyl-alpha-boswellic acid are depicted for the first time.</dc:description>
  <dc:identifier>https://sonar.ch/global/documents/93568</dc:identifier>
  <dc:language>eng</dc:language>
  <dc:relation>info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fitote.2020.104694</dc:relation>
  <dc:relation>info:eu-repo/semantics/altIdentifier/pmid/32712132</dc:relation>
  <dc:source>Fitoterapia. - 2020</dc:source>
  <dc:subject xmlns:ns1="xml" ns1:lang="en">3-O-acetyl-α-boswellic acid</dc:subject>
  <dc:subject xmlns:ns2="xml" ns2:lang="en">Boswellia</dc:subject>
  <dc:subject xmlns:ns3="xml" ns3:lang="en">Immunosuppression</dc:subject>
  <dc:subject xmlns:ns4="xml" ns4:lang="en">Interleukin-2</dc:subject>
  <dc:subject xmlns:ns5="xml" ns5:lang="en">NFAT</dc:subject>
  <dc:subject xmlns:ns6="xml" ns6:lang="en">T cell signaling</dc:subject>
  <dc:title xmlns:ns7="xml" ns7:lang="en">Boswellia carteri extract and 3-O-acetyl-alpha-boswellic acid suppress T cell function.</dc:title>
  <dc:type>http://purl.org/coar/resource_type/c_6501</dc:type>
</oai_dc:dc>