Béguin P Crambert G Monnet-Tschudi F Uldry M Horisberger JD Garty H Geering K 2002 Recently, corticosteroid hormone-induced factor (CHIF) and the gamma-subunit, two members of the FXYD family of small proteins, have been identified as regulators of renal Na,K-ATPase. In this study, we have investigated the tissue distribution and the structural and functional properties of FXYD7, another family member which has not yet been characterized. Expressed exclusively in the brain, FXYD7 is a type I membrane protein bearing N-terminal, post-translationally added modifications on threonine residues, most probably O-glycosylations that are important for protein stabilization. Expressed in Xenopus oocytes, FXYD7 can interact with Na,K-ATPase alpha 1-beta 1, alpha 2-beta 1 and alpha 3-beta 1 but not with alpha-beta 2 isozymes, whereas, in brain, it is only associated with alpha 1-beta isozymes. FXYD7 decreases the apparent K(+) affinity of alpha 1-beta 1 and alpha 2-beta 1, but not of alpha 3-beta1 isozymes. These data suggest that FXYD7 is a novel, tissue- and isoform-specific Na,K-ATPase regulator which could play an important role in neuronal excitability. application/pdf https://sonar.ch/global/documents/93594 eng info:eu-repo/semantics/altIdentifier/doi/10.1093/emboj/cdf330 info:eu-repo/semantics/altIdentifier/pmid/12093728 info:eu-repo/semantics/openAccess The EMBO journal. - 2002 Action Potentials Animals Brain Chemistry Brefeldin A DNA, Complementary Glycosylation Isoenzymes Kidney Membrane Glycoproteins Mice Microsomes Mutagenesis, Site-Directed Nerve Tissue Proteins Neurons Oocytes Organ Specificity Potassium Protein Interaction Mapping Protein Processing, Post-Translational Protein Subunits RNA, Messenger Rats Recombinant Fusion Proteins Sodium-Potassium-Exchanging ATPase Structure-Activity Relationship Xenopus laevis FXYD7 is a brain-specific regulator of Na,K-ATPase alpha 1-beta isozymes. http://purl.org/coar/resource_type/c_6501