<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:creator>Gallaud E</dc:creator>
  <dc:creator>Ramdas Nair A</dc:creator>
  <dc:creator>Horsley N</dc:creator>
  <dc:creator>Monnard A</dc:creator>
  <dc:creator>Singh P</dc:creator>
  <dc:creator>Pham TT</dc:creator>
  <dc:creator>Salvador Garcia D</dc:creator>
  <dc:creator>Ferrand A</dc:creator>
  <dc:creator>Cabernard C</dc:creator>
  <dc:date>2020</dc:date>
  <dc:description xmlns:ns0="xml" ns0:lang="en">Centrosomes, the main microtubule organizing centers (MTOCs) of metazoan cells, contain an older "mother" and a younger "daughter" centriole. Stem cells either inherit the mother or daughter-centriole-containing centrosome, providing a possible mechanism for biased delivery of cell fate determinants. However, the mechanisms regulating centrosome asymmetry and biased centrosome segregation are unclear. Using 3D-structured illumination microscopy (3D-SIM) and live-cell imaging, we show in fly neural stem cells (neuroblasts) that the mitotic kinase Polo and its centriolar protein substrate Centrobin (Cnb) accumulate on the daughter centriole during mitosis, thereby generating molecularly distinct mother and daughter centrioles before interphase. Cnb's asymmetric localization, potentially involving a direct relocalization mechanism, is regulated by Polo-mediated phosphorylation, whereas Polo's daughter centriole enrichment requires both Wdr62 and Cnb. Based on optogenetic protein mislocalization experiments, we propose that the establishment of centriole asymmetry in mitosis primes biased interphase MTOC activity, necessary for correct spindle orientation.</dc:description>
  <dc:format>application/pdf</dc:format>
  <dc:identifier>https://sonar.ch/global/documents/93635</dc:identifier>
  <dc:language>eng</dc:language>
  <dc:relation>info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pbio.3000762</dc:relation>
  <dc:relation>info:eu-repo/semantics/altIdentifier/pmid/32760088</dc:relation>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:source>PLoS biology. - 2020</dc:source>
  <dc:subject xmlns:ns1="xml" ns1:lang="en">Animals</dc:subject>
  <dc:subject xmlns:ns2="xml" ns2:lang="en">Animals, Genetically Modified</dc:subject>
  <dc:subject xmlns:ns3="xml" ns3:lang="en">Bacterial Proteins</dc:subject>
  <dc:subject xmlns:ns4="xml" ns4:lang="en">Cell Cycle Proteins</dc:subject>
  <dc:subject xmlns:ns5="xml" ns5:lang="en">Centrioles</dc:subject>
  <dc:subject xmlns:ns6="xml" ns6:lang="en">Centrosome</dc:subject>
  <dc:subject xmlns:ns7="xml" ns7:lang="en">Drosophila Proteins</dc:subject>
  <dc:subject xmlns:ns8="xml" ns8:lang="en">Drosophila melanogaster</dc:subject>
  <dc:subject xmlns:ns9="xml" ns9:lang="en">Embryo, Nonmammalian</dc:subject>
  <dc:subject xmlns:ns10="xml" ns10:lang="en">Gene Expression Regulation, Developmental</dc:subject>
  <dc:subject xmlns:ns11="xml" ns11:lang="en">Genes, Reporter</dc:subject>
  <dc:subject xmlns:ns12="xml" ns12:lang="en">Green Fluorescent Proteins</dc:subject>
  <dc:subject xmlns:ns13="xml" ns13:lang="en">Interphase</dc:subject>
  <dc:subject xmlns:ns14="xml" ns14:lang="en">Luminescent Proteins</dc:subject>
  <dc:subject xmlns:ns15="xml" ns15:lang="en">Mitosis</dc:subject>
  <dc:subject xmlns:ns16="xml" ns16:lang="en">Nerve Tissue Proteins</dc:subject>
  <dc:subject xmlns:ns17="xml" ns17:lang="en">Neural Stem Cells</dc:subject>
  <dc:subject xmlns:ns18="xml" ns18:lang="en">Optogenetics</dc:subject>
  <dc:subject xmlns:ns19="xml" ns19:lang="en">Phosphorylation</dc:subject>
  <dc:subject xmlns:ns20="xml" ns20:lang="en">Protein-Serine-Threonine Kinases</dc:subject>
  <dc:subject xmlns:ns21="xml" ns21:lang="en">Signal Transduction</dc:subject>
  <dc:title xmlns:ns22="xml" ns22:lang="en">Dynamic centriolar localization of Polo and Centrobin in early mitosis primes centrosome asymmetry.</dc:title>
  <dc:type>http://purl.org/coar/resource_type/c_6501</dc:type>
</oai_dc:dc>