Thoms M Mitterer V Kater L Falquet L Beckmann R Kressler D Hurt E 2018 During eukaryotic 60S biogenesis, the 5S RNP requires a large rotational movement to achieve its mature position. Cryo-EM of the Rix1-Rea1 pre-60S particle has revealed the post-rotation stage, in which a gently undulating α-helix corresponding to Cgr1 becomes wedged between Rsa4 and the relocated 5S RNP, but the purpose of this insertion was unknown. Here, we show that cgr1 deletion in yeast causes a slow-growth phenotype and reversion of the pre-60S particle to the pre-rotation stage. However, spontaneous extragenic suppressors could be isolated, which restore growth and pre-60S biogenesis in the absence of Cgr1. Whole-genome sequencing reveals that the suppressor mutations map in the Rpf2-Rrs1 module and Rpl5, which together stabilize the unrotated stage of the 5S RNP. Thus, mutations in factors stabilizing the pre-rotation stage facilitate 5S RNP relocation upon deletion of Cgr1, but Cgr1 itself could stabilize the post-rotation stage. application/pdf https://sonar.ch/global/documents/93864 eng info:eu-repo/semantics/altIdentifier/doi/10.1038/s41467-018-06660-w info:eu-repo/semantics/altIdentifier/pmid/30291245 info:eu-repo/semantics/openAccess Nature communications. - 2018 Amino Acid Sequence Nuclear Proteins RNA, Ribosomal, 5S RNA-Binding Proteins Ribosomal Proteins Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Suppression, Genetic Suppressor mutations in Rpf2-Rrs1 or Rpl5 bypass the Cgr1 function for pre-ribosomal 5S RNP-rotation. http://purl.org/coar/resource_type/c_6501