<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:creator>Kamiya M</dc:creator>
  <dc:creator>Johnsson K</dc:creator>
  <dc:date>2010</dc:date>
  <dc:description xmlns:ns0="xml" ns0:lang="en">We introduce here a new class of BODIPY-based Ca2+ indicators which can be derivatized with biological ligands that permit the localization of the indicators in living cells. The underivatized BODIPY-based Ca2+ indicator (BOCA-1) shows a 250-fold increase in fluorescence intensity upon Ca2+ binding. We also prepared its O6-benzylguanine (BG) derivative, BOCA-1-BG, which can be covalently and specifically linked to SNAP-tag fusion proteins in living cells. The indicator retains its properties as a highly sensitive Ca2+ indicator after conjugation to proteins, displaying a 180-fold increase in fluorescence intensity upon Ca2+ binding. We further demonstrated that BOCA-1-BG through reaction with localized SNAP-tag fusion proteins can be used to sense changes in Ca2+ concentrations in the nuclei and in the cytosol of live CHO-K1 cells. The high sensitivity of the indicator together with the possibility to selectively couple it to proteins of interest makes it a powerful tool for measuring local changes in Ca2+ concentrations in living cells.</dc:description>
  <dc:identifier>https://sonar.ch/global/documents/96891</dc:identifier>
  <dc:language>eng</dc:language>
  <dc:relation>info:eu-repo/semantics/altIdentifier/doi/10.1021/ac100741t</dc:relation>
  <dc:relation>info:eu-repo/semantics/altIdentifier/pmid/20590099</dc:relation>
  <dc:source>Analytical chemistry. - 2010</dc:source>
  <dc:subject xmlns:ns1="xml" ns1:lang="en">Animals</dc:subject>
  <dc:subject xmlns:ns2="xml" ns2:lang="en">Boron Compounds</dc:subject>
  <dc:subject xmlns:ns3="xml" ns3:lang="en">CHO Cells</dc:subject>
  <dc:subject xmlns:ns4="xml" ns4:lang="en">Calcium</dc:subject>
  <dc:subject xmlns:ns5="xml" ns5:lang="en">Cricetinae</dc:subject>
  <dc:subject xmlns:ns6="xml" ns6:lang="en">Cricetulus</dc:subject>
  <dc:subject xmlns:ns7="xml" ns7:lang="en">Fluorescent Dyes</dc:subject>
  <dc:subject xmlns:ns8="xml" ns8:lang="en">Glycine</dc:subject>
  <dc:subject xmlns:ns9="xml" ns9:lang="en">Guanine</dc:subject>
  <dc:subject xmlns:ns10="xml" ns10:lang="en">Indicators and Reagents</dc:subject>
  <dc:subject xmlns:ns11="xml" ns11:lang="en">Microscopy, Fluorescence</dc:subject>
  <dc:subject xmlns:ns12="xml" ns12:lang="en">Purines</dc:subject>
  <dc:title xmlns:ns13="xml" ns13:lang="en">Localizable and highly sensitive calcium indicator based on a BODIPY fluorophore.</dc:title>
  <dc:type>http://purl.org/coar/resource_type/c_6501</dc:type>
</oai_dc:dc>