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Environmental factors determining distribution and activity of anammox bacteria in minerotrophic fen soils.
Journal article

Environmental factors determining distribution and activity of anammox bacteria in minerotrophic fen soils.

  • Bagnoud A Laboratory of Microbiology, University of Neuchâtel, Rue Emile-Argand 11, CH-2009 Neuchâtel, Switzerland.
  • Guye-Humbert S Laboratory of Microbiology, University of Neuchâtel, Rue Emile-Argand 11, CH-2009 Neuchâtel, Switzerland.
  • Schloter-Hai B Research Unit for Comparative Microbiome Analysis; Helmholtz Zentrum München, Ingolstädter Landstraße 1, D-85764 Neuherberg, Germany.
  • Schloter M Research Unit for Comparative Microbiome Analysis; Helmholtz Zentrum München, Ingolstädter Landstraße 1, D-85764 Neuherberg, Germany.
  • Zopfi J Laboratory of Microbiology, University of Neuchâtel, Rue Emile-Argand 11, CH-2009 Neuchâtel, Switzerland.
  • 2019-11-30
Published in:
  • FEMS microbiology ecology. - 2020
ammonia oxidation
ecotone
generalized linear model
nitrogen cycling
wetland
Ammonia
Anaerobiosis
Bacteria
Nitrification
Nitrogen
Oxidation-Reduction
RNA, Ribosomal, 16S
Soil Microbiology
English In contrast to the pervasive occurrence of denitrification in soils, anammox (anaerobic ammonium oxidation) is a spatially restricted process that depends on specific ecological conditions. To identify the factors that constrain the distribution and activity of anammox bacteria in terrestrial environments, we investigated four different soil types along a catena with opposing ecological gradients of nitrogen and water content, from an amended pasture to an ombrotrophic bog. Anammox was detected by polymerase chain reaction (PCR) and quantitative PCR (qPCR) only in the nitrophilic wet meadow and the minerotrophic fen, in soil sections remaining water-saturated for most of the year and whose interstitial water contained inorganic nitrogen. Contrastingly, aerobic ammonia oxidizing microorganisms were present in all examined samples and outnumbered anammox bacteria usually by at least one order of magnitude. 16S rRNA gene sequencing revealed a relatively high diversity of anammox bacteria with one Ca. Brocadia cluster. Three additional clusters could not be affiliated to known anammox genera, but have been previously detected in other soil systems. Soil incubations using 15N-labeled substrates revealed that anammox processes contributed about <2% to total N2 formation, leaving nitrification and denitrification as the dominant N-removal mechanism in these soils that represent important buffer zones between agricultural land and ombrotrophic peat bogs.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/231831
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