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Lymphoma and Leukemia Cell Vulnerabilities and Resistance Identified by Compound Library Screens.
Journal article

Lymphoma and Leukemia Cell Vulnerabilities and Resistance Identified by Compound Library Screens.

  • Tomska K Molecular Therapy in Haematology and Oncology, National Center for Tumor Diseases, German Cancer Research Center (DKFZ), Heidelberg, Germany.
  • Scheinost S Molecular Therapy in Haematology and Oncology, National Center for Tumor Diseases, German Cancer Research Center (DKFZ), Heidelberg, Germany.
  • Zenz T Department of Haematology, Center for Oncology and Haematology, University Hospital and University of Zurich, Zurich, Switzerland. Thorsten.zenz@usz.ch.
  • 2019-02-20
Published in:
  • Methods in molecular biology (Clifton, N.J.). - 2019
Assay plates
Drug library
Drug response profiling
Genomics of drug response
Leukemia
Lymphoma
Antineoplastic Agents
Biomarkers, Pharmacological
Cell Culture Techniques
Cell Line, Tumor
Cell Survival
Drug Resistance, Neoplasm
Drug Screening Assays, Antitumor
High-Throughput Screening Assays
Humans
Leukemia
Lymphoma
Tumor Cells, Cultured
English Response to anticancer agents is often restricted to subsets of patients. The recognition of factors underlying this heterogeneity and the identification of biomarkers associated with response to drugs would greatly improve the efficacy of drug treatment. Platforms that can comprehensively map drug response in high-throughput ex vivo provide a unique tool to identify associated biomarkers and provide hypotheses for mechanisms underlying variable response. Such screens can be performed on cell lines and short-term cultures of primary cells to take advantage of the respective models' strength, which include, e.g., the ability to silence genes in cell lines and the "indefinite" supply of primary cells where clonal selection can be avoided. Cohorts of such samples represent the natural diversity of cancers, including rarer mutations and combinatorial patterns of mutations.We here summarize a simple and scalable method for the measurement of viability after drug exposure based on ATP measurements as a surrogate for viability, which we use to measure and understand drug response in cell lines and primary cells.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/47486
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