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The complete mitochondrial genome of the invasive Ponto-Caspian goby Ponticola kessleri obtained from high-throughput sequencing using the Ion Torrent Personal Genome Machine.
Journal article

The complete mitochondrial genome of the invasive Ponto-Caspian goby Ponticola kessleri obtained from high-throughput sequencing using the Ion Torrent Personal Genome Machine.

  • Kalchhauser I a Department of Environmental Science , Programm MGU, University of Basel , Basel , Switzerland and.
  • Kutschera VE b Biodiversität und Klima Forschungszentrum , Senckenberganlage 25 , Frankfurt am Main , Germany.
  • Burkhardt-Holm P a Department of Environmental Science , Programm MGU, University of Basel , Basel , Switzerland and.
  • 2014-10-21
Published in:
  • Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis. - 2016
Bighead goby
Ponticola kessleri
Rhine
high throughput sequencing
invasive
mitochondrial genome
Animals
Base Composition
Base Pairing
DNA, Mitochondrial
Genes, Mitochondrial
Genome, Mitochondrial
High-Throughput Nucleotide Sequencing
Perciformes
RNA, Transfer
English We report the first complete mitochondrial genome (mitogenome) of an invasive Ponto-Caspian goby, Ponticola kessleri (bighead goby, Günther 1891). Ion Torrent PGM sequencing of total DNA from two individuals yielded a contig of 16,971 bp, with overlapping ends located in the repetitive control region, which was validated using Sanger sequencing. The final mitogenome of Ponticola kessleri has a size of 16,890 bp and contains the expected gene configuration of 13 protein-coding genes, 2 rRNA genes and 22 tRNA genes. In a comparison with complete mitogenomes from other goby species, we identified a translocation of tRNA-Glu in the mitogenome of P. kessleri. Rearrangements are unique and rare events, and can thus provide phylogenetic information.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/53429
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