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In vivo evaluation of the biocompatibility and biodegradation of a new denatured plasma membrane combined with liquid PRF (Alb-PRF).
Journal article

In vivo evaluation of the biocompatibility and biodegradation of a new denatured plasma membrane combined with liquid PRF (Alb-PRF).

  • Gheno E Post-Graduation Program in Dentistry, Fluminense Federal University , Niterói, Brazil.
  • Mourão CFAB Post-Graduation Program in Dentistry, Fluminense Federal University , Niterói, Brazil.
  • Mello-Machado RC Post-Graduation Program in Dentistry, Fluminense Federal University , Niterói, Brazil.
  • Stellet Lourenço E Post-Graduation Program in Dentistry, Fluminense Federal University , Niterói, Brazil.
  • Miron RJ Department of Periodontology, University of Bern , Bern, Switzerland.
  • Catarino KFF Undergraduate Student, Dentistry School, Fluminense Federal University , Niterói, Brazil.
  • Alves AT Department of Oral Pathology, Dentistry School, Fluminense Federal University , Niterói, Brazil.
  • Alves GG Department of Molecular and Cell Biology, Institute of Biology, Fluminense Federal University , Niterói, Brazil.
  • Calasans-Maia MD Oral Surgery Department, Dentistry School, Fluminense Federal University , Niterói, Brazil.
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  • 2020-06-13
Published in:
  • Platelets. - 2020
Alb-PRF
albumin
extended-PRF
horizontal centrifugation
platelet-rich fibrin
English Guided bone regeneration (GBR) is a process that involves the regeneration of bone defects through the application of occlusive membranes that mechanically exclude the population of non-osteogenic cells from the surrounding soft tissue. Interestingly, platelet-rich fibrin (PRF) has previously been proposed as an autologous GBR membrane despite its short-term resorption period of 2-3 weeks. Recent clinical observations have demonstrated that, by heating a liquid platelet-poor plasma (PPP) layer and mixing the cell-rich buffy coat zone, the resorption properties of heated albumin gel with liquid-PRF (Alb-PRF) can be significantly improved. The aim of this study was to evaluate the inflammatory reaction, biocompatibility, and extended degradation properties of a new autologous Alb-PRF membrane in comparison to commonly utilized standard PRF after nude mice implantation, according to ISO 10993-6/2016. Two standard preparations of PRF (L-PRF and H-PRF) were compared to novel Alb-PRF following subcutaneous implantation at 7, 14, and 21 days. All groups demonstrated excellent biocompatibility owing to their autologous sources. However, it is worth noting that, while both L-PRF and H-PRF membranes demonstrated significant or complete resorption by 21 days, the Alb-PRF membrane remained volume-stable throughout the duration of the study. This study demonstrates-for the first time, to the best of our knowledge-a marked improvement in the membrane stability of Alb-PRF. This indicates its future potential for use as a biological barrier membrane for GBR procedures with a long-lasting half-life, or as a biological filler material in esthetic medicine applications. Thus, further studies are warranted to explore future clinical applications in various fields of medicine.
Language
  • English
Open access status
closed
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Persistent URL
https://sonar.ch/global/documents/93
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