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An In Vitro Lung System to Assess the Proinflammatory Hazard of Carbon Nanotube Aerosols.

  • Barosova H BioNanomaterials Group, Adolphe Merkle Institute, University of Fribourg, 1700 Fribourg, Switzerland.
  • Karakocak BB BioNanomaterials Group, Adolphe Merkle Institute, University of Fribourg, 1700 Fribourg, Switzerland.
  • Septiadi D BioNanomaterials Group, Adolphe Merkle Institute, University of Fribourg, 1700 Fribourg, Switzerland.
  • Petri-Fink A BioNanomaterials Group, Adolphe Merkle Institute, University of Fribourg, 1700 Fribourg, Switzerland.
  • Stone V Institute of Biological Chemistry, Biophysics and Bioengineering, Heriot-Watt University, Edinburgh EH14 4AS, UK.
  • Rothen-Rutishauser B BioNanomaterials Group, Adolphe Merkle Institute, University of Fribourg, 1700 Fribourg, Switzerland.
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  • 2020-07-31
Published in:
  • International journal of molecular sciences. - 2020
air-liquid interface
carbon nanotubes
co-culture
in vitro
lung
multiwalled carbon nanotubes
profibrotic
proinflammatory
toxicity
English In vitro three-dimensional (3D) lung cell models have been thoroughly investigated in recent years and provide a reliable tool to assess the hazard associated with nanomaterials (NMs) released into the air. In this study, a 3D lung co-culture model was optimized to assess the hazard potential of multiwalled carbon nanotubes (MWCNTs), which is known to provoke inflammation and fibrosis, critical adverse outcomes linked to acute and prolonged NM exposure. The lung co-cultures were exposed to MWCNTs at the air-liquid interface (ALI) using the VITROCELL® Cloud system while considering realistic occupational exposure doses. The co-culture model was composed of three human cell lines: alveolar epithelial cells (A549), fibroblasts (MRC-5), and macrophages (differentiated THP-1). The model was exposed to two types of MWCNTs (Mitsui-7 and Nanocyl) at different concentrations (2-10 μg/cm2) to assess the proinflammatory as well as the profibrotic responses after acute (24 h, one exposure) and prolonged (96 h, repeated exposures) exposure cycles. The results showed that acute or prolonged exposure to different concentrations of the tested MWCNTs did not induce cytotoxicity or apparent profibrotic response; however, suggested the onset of proinflammatory response.
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  • English
Open access status
gold
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https://sonar.ch/global/documents/93367
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