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Label-free detection of hypoxia-induced extracellular vesicle secretion from MCF-7 cells.

  • Kilic T Swiss Federal Institute of Technology Lausanne, EPFL, Integrated Systems Laboratory (LSI), 1015, Lausanne, Switzerland. tugba.kilic@epfl.ch.
  • Valinhas ATS Swiss Federal Institute of Technology Lausanne, EPFL, Integrated Systems Laboratory (LSI), 1015, Lausanne, Switzerland.
  • Wall I Department of Biochemical Engineering, University College London, Bernard Katz Building, Gordon Street, WC1H 0AH, London, England.
  • Renaud P Swiss Federal Institute of Technology Lausanne, EPFL, Microsystems Laboratory 4 (LMIS4), 1015, Lausanne, Switzerland.
  • Carrara S Swiss Federal Institute of Technology Lausanne, EPFL, Integrated Systems Laboratory (LSI), 1015, Lausanne, Switzerland.
  • 2018-06-22
Published in:
  • Scientific reports. - 2018
Cell Hypoxia
Dielectric Spectroscopy
Enzyme-Linked Immunosorbent Assay
Extracellular Vesicles
Humans
MCF-7 Cells
English Nanoscale extracellular vesicles (EVs) including exosomes (50-150 nm membrane particles) have emerged as promising cancer biomarkers due to the carried genetic information about the parental cells. However the sensitive detection of these vesicles remains a challenge. Here we present a label-free electrochemical sensor to measure the EVs secretion levels of hypoxic and normoxic MCF-7 cells. The sensor design includes two consecutive steps; i) Au electrode surface functionalization for anti-CD81 Antibody and ii) EVs capture. The label-free detection of EVs was done via Differential Pulse Voltammetry (DPV) and Electrochemical Impedance Spectroscopy (EIS). The working linear range for the sensor was 102-109 EVs/ml with an LOD 77 EVs/mL and 379 EVs/ml for EIS and DPV based detection. A blood-abundant protein, RhD was used for the selectivity test. In order to assess the performance of the biosensor, the level of EVs secretion by the human breast cancer MCF-7 cell line was compared with enzyme-linked immunosorbent assays (ELISA) and Nanoparticle Tracking Analysis (NTA). Designed label-free electrochemical sensors utilized for quantification of EVs secretion enhancement due to CoCl2-induced hypoxia and 1.23 fold increase with respect to normoxic conditions was found.
Language
  • English
Open access status
gold
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https://sonar.ch/global/documents/99583
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