2026-11-03T07:36:57Z https://sonar.ch/oai2d

oai:sonar.ch:5235 2025-10-24T20:14:22Z

Bellée V Pais P Puig Navarro A Blanc F Schneider O Trabelsi K Veneziano G 2019 A method is proposed to measure the photon polarisation parameter in transitions using an amplitude analysis of decays. Simplified models of the system are used to simulate and decays, validate the amplitude analysis method, and demonstrate the feasibility of a measurement of the parameter irrespective of the model parameters. Similar sensitivities to are obtained with both the charged and neutral hadronic systems. In the absence of any background and distortion due to experimental effects, the statistical uncertainty expected from an analysis of decays in an LHCb data set corresponding to an integrated luminosity of 9  is estimated to be 0.009. A similar measurement using decays in a Belle II data sample corresponding to an integrated luminosity of 5  would lead to a statistical uncertainty of 0.018. application/pdf https://sonar.ch/global/documents/5235 eng info:eu-repo/semantics/altIdentifier/doi/10.1140/epjc/s10052-019-7127-3 info:eu-repo/semantics/altIdentifier/pmid/31402844 info:eu-repo/semantics/openAccess The European physical journal. C, Particles and fields. - 2019 Physics and Astronomy (miscellaneous) Engineering (miscellaneous) Using an amplitude analysis to measure the photon polarisation in decays. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:5277 2025-10-24T20:14:23Z

Okonska A Bühler S Rao V Ronner M Blijlevens M van der Meulen-Muileman IH de Menezes RX Wipplinger M Oehl K Smit EF Weder W Stahel RA Penengo L van Beusechem VW Felley-Bosco E 2020 Loss of function of BRCA1-associated protein 1 (BAP1) is observed in about 50% of malignant pleural mesothelioma (MPM) cases. The aim of this study was to investigate whether this aspect could be exploited for targeted therapy. A genetically engineered model was established expressing either functional or nonfunctional BAP1, and whole-genome siRNA synthetic lethality screens were performed assessing differentially impaired survival between the two cell lines. The whole-genome siRNA screen unexpectedly revealed 11 hits (FDR < 0.05) that were more cytotoxic to BAP1-proficient cells. Two actionable targets, ribonucleotide reductase (RNR) catalytic subunit M1 (RRM1) and RNR regulatory subunit M2 (RRM2), were validated. In line with the screen results, primary mesothelioma (BAP1 +/-) overexpressing BAP1 C91A (catalytically dead mutant) was more resistant to RNR inhibition, while BAP1 knockdown in the BAP1-proficient cell lines rescued the cells from their vulnerability to RNR depletion. Gemcitabine and hydroxyurea were more cytotoxic in BAP1-proficient cell line-derived spheroids compared with BAP1 deficient. Upregulation of RRM2 upon gemcitabine and hydroxyurea treatment was more profound in BAP1 mut/del cell lines. Increased lethality mediated by RNR inhibition was observed in NCI-H2452 cells reconstituted with BAP1-WT but not with BAP1 C91A. Upregulation of RRM2 in NCI-H2452-BAP1 WT spheroids was modest compared with control or C91A mutant. Together, we found that BAP1 is involved in the regulation of RNR levels during replication stress. Our observations reveal a potential clinical application where BAP1 status could serve as predictive or stratification biomarker for RNR inhibition-based therapy in MPM. application/pdf https://sonar.ch/global/documents/5277 eng info:eu-repo/semantics/altIdentifier/doi/10.1158/1535-7163.MCT-19-0356 info:eu-repo/semantics/altIdentifier/pmid/31619462 info:eu-repo/semantics/openAccess Molecular cancer therapeutics. - 2020 Cancer Research Oncology Functional Genomic Screen in Mesothelioma Reveals that Loss of Function of BRCA1-Associated Protein 1 Induces Chemoresistance to Ribonucleotide Reductase Inhibition. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:5312 2025-10-24T20:14:23Z

Felley-Bosco E 2019 application/pdf https://sonar.ch/global/documents/5312 eng info:eu-repo/semantics/altIdentifier/doi/10.3389/fgene.2019.01121 info:eu-repo/semantics/altIdentifier/pmid/31788004 info:eu-repo/semantics/openAccess Frontiers in genetics. - 2019 Gli-1 inhibition hedgehog inhibition hedgehog signaling macrophage differentiation malignant pleural mesothelioma personalized medicine tumor-stroma cross talk Hedgehog Signaling in Mesothelioma: 2019 Status. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:5387 2025-10-24T20:14:25Z

Martin-Isla C Campello VM Izquierdo C Raisi-Estabragh Z Baeßler B Petersen SE Lekadir K 2020 Cardiac imaging plays an important role in the diagnosis of cardiovascular disease (CVD). Until now, its role has been limited to visual and quantitative assessment of cardiac structure and function. However, with the advent of big data and machine learning, new opportunities are emerging to build artificial intelligence tools that will directly assist the clinician in the diagnosis of CVDs. This paper presents a thorough review of recent works in this field and provide the reader with a detailed presentation of the machine learning methods that can be further exploited to enable more automated, precise and early diagnosis of most CVDs. application/pdf https://sonar.ch/global/documents/5387 eng info:eu-repo/semantics/altIdentifier/doi/10.3389/fcvm.2020.00001 info:eu-repo/semantics/altIdentifier/pmid/32039241 info:eu-repo/semantics/openAccess Frontiers in cardiovascular medicine. - 2020 artificial intelligence automated diagnosis cardiac imaging cardiovascular disease deep learning machine learning radiomics Image-Based Cardiac Diagnosis With Machine Learning: A Review. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:4967 2025-10-24T20:14:26Z

Kishore S Jaskiewicz L Burger L Hausser J Khorshid M Zavolan M 2011 Cross-linking and immunoprecipitation (CLIP) is increasingly used to map transcriptome-wide binding sites of RNA-binding proteins. We developed a method for CLIP data analysis, and applied it to compare CLIP with photoactivatable ribonucleoside-enhanced CLIP (PAR-CLIP) and to uncover how differences in cross-linking and ribonuclease digestion affect the identified sites. We found only small differences in accuracies of these methods in identifying binding sites of HuR, which binds low-complexity sequences, and Argonaute 2, which has a complex binding specificity. We found that cross-link-induced mutations led to single-nucleotide resolution for both PAR-CLIP and CLIP. Our results confirm the expectation from original CLIP publications that RNA-binding proteins do not protect their binding sites sufficiently under the denaturing conditions used during the CLIP procedure, and we show that extensive digestion with sequence-specific RNases strongly biases the recovered binding sites. This bias can be substantially reduced by milder nuclease digestion conditions. https://sonar.ch/global/documents/4967 eng info:eu-repo/semantics/altIdentifier/doi/10.1038/nmeth.1608 info:eu-repo/semantics/altIdentifier/pmid/21572407 Nature methods. - 2011 Binding Sites Cross-Linking Reagents Immunoprecipitation RNA-Binding Proteins A quantitative analysis of CLIP methods for identifying binding sites of RNA-binding proteins. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:4995 2025-10-24T20:14:29Z

Abel S Chien P Wassmann P Schirmer T Kaever V Laub MT Baker TA Jenal U 2011 In Caulobacter crescentus, phosphorylation of key regulators is coordinated with the second messenger cyclic di-GMP to drive cell-cycle progression and differentiation. The diguanylate cyclase PleD directs pole morphogenesis, while the c-di-GMP effector PopA initiates degradation of the replication inhibitor CtrA by the AAA+ protease ClpXP to license S phase entry. Here, we establish a direct link between PleD and PopA reliant on the phosphodiesterase PdeA and the diguanylate cyclase DgcB. PdeA antagonizes DgcB activity until the G1-S transition, when PdeA is degraded by the ClpXP protease. The unopposed DgcB activity, together with PleD activation, upshifts c-di-GMP to drive PopA-dependent CtrA degradation and S phase entry. PdeA degradation requires CpdR, a response regulator that delivers PdeA to the ClpXP protease in a phosphorylation-dependent manner. Thus, CpdR serves as a crucial link between phosphorylation pathways and c-di-GMP metabolism to mediate protein degradation events that irreversibly and coordinately drive bacterial cell-cycle progression and development. application/pdf https://sonar.ch/global/documents/4995 eng info:eu-repo/semantics/altIdentifier/doi/10.1016/j.molcel.2011.07.018 info:eu-repo/semantics/altIdentifier/pmid/21855795 info:eu-repo/semantics/openAccess Molecular cell. - 2011 Bacterial Proteins Caulobacter crescentus Cell Cycle Cell Polarity Models, Biological Phosphoric Diester Hydrolases Phosphorylation Second Messenger Systems Regulatory cohesion of cell cycle and cell differentiation through interlinked phosphorylation and second messenger networks. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:5008 2025-10-24T20:14:30Z

Kossowsky J Wilhelm FH Roth WT Schneider S 2012 BACKGROUND Separation anxiety disorder (SAD) is one of the most common anxiety disorders in childhood and is predictive of adult anxiety disorders, especially panic disorder. However, the disorder has seldom been studied and the attempt to distinguish SAD from other anxiety disorders with regard to psychophysiology has not been made. We expected exaggerated anxiety as well as sympathetic and respiratory reactivity in SAD during separation from the mother. METHOD Participants were 49 children with a principal diagnosis of SAD, 21 clinical controls (CC) with a principal diagnosis of anxiety disorder other than SAD, and 39 healthy controls (HC) not meeting criteria for any current diagnosis. Analyses of covariance controlling for age were used to assess sympathetic and parasympathetic activation (preejection period and respiratory sinus arrhythmia) as well as cardiovascular (heart rate, mean arterial pressure, total peripheral resistance), respiratory (total breath time, minute ventilation, tidal volume, end-tidal CO(2) , respiratory variability), electrodermal, and self-report (anxiety, cognitions, symptoms) variables during baseline, 4-min separation from, and reunion with the mother. RESULTS Children with a diagnosis of SAD were characterized by elevated self-reported anxiety responses to separation and increased sympathetic reactivity compared with CC and HC groups. The SAD group also displayed greater vagal withdrawal and higher reactivity in multiple cardiovascular, respiratory, and electrodermal measures compared with the HC group, while corresponding responses were less in the CC group and not significantly different from the other groups. CONCLUSIONS Separation from the mother elicits greater autonomic, respiratory, and experiential responses in children with SAD. Our findings based on brief experimental separation demonstrate differential subjective and physiological manifestations of specific anxiety diagnoses, thus supporting the validity of the diagnostic category of SAD. https://sonar.ch/global/documents/5008 eng info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1469-7610.2011.02465.x info:eu-repo/semantics/altIdentifier/pmid/21923807 Journal of child psychology and psychiatry, and allied disciplines. - 2012 Adolescent Adult Anxiety Disorders Anxiety, Separation Autonomic Nervous System Blood Pressure Capnography Child Child, Preschool Electrocardiography Female Galvanic Skin Response Humans Male Mothers Psychiatric Status Rating Scales Respiration Self Report Stress, Psychological Separation anxiety disorder in children: disorder-specific responses to experimental separation from the mother. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:5014 2025-10-24T20:14:31Z

Flicker F Betz G 2012 UNLABELLED The aim of this study was to develop a high-dose tablet formulation of the poorly soluble carbamazepine (CBZ) with sufficient tablet hardness and immediate drug release. A further aim was to investigate the influence of various commercial CBZ raw materials on the optimized tablet formulation. MATERIALS AND METHODS Hydroxypropyl cellulose (HPC-SL) was selected as a dry binder and crospovidone (CrosPVP) as a superdisintegrant. A direct compacted tablet formulation of 70% CBZ was optimized by a 3² full factorial design with two input variables, HPC (0--10%) and CrosPVP (0--5%). Response variables included disintegration time, amount of drug released at 15 and 60 min, and tablet hardness, all analyzed according to USP 31. RESULTS AND DISCUSSION Increasing HPC-SL together with CrosPVP not only increased tablet hardness but also reduced disintegration time. Optimal condition was achieved in the range of 5--9% HPC and 3--5% CrosPVP, where tablet properties were at least 70 N tablet hardness, less than 1 min disintegration, and within the USP requirements for drug release. Testing the optimized formulation with four different commercial CBZ samples, their variability was still observed. Nonetheless, all formulations conformed to the USP specifications. CONCLUSIONS With the excipients CrosPVP and HPC-SL an immediate release tablet formulation was successfully formulated for high-dose CBZ of various commercial sources. https://sonar.ch/global/documents/5014 eng info:eu-repo/semantics/altIdentifier/doi/10.3109/03639045.2011.623166 info:eu-repo/semantics/altIdentifier/pmid/22010838 Drug development and industrial pharmacy. - 2012 Analysis of Variance Anticonvulsants Carbamazepine Cellulose Excipients Hardness Particle Size Povidone Solubility Tablets Effect of crospovidone and hydroxypropyl cellulose on carbamazepine in high-dose tablet formulation. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:5016 2025-10-24T20:14:31Z

Scherer K Schindler C Arnold A Bach S Bircher AJ 2012 Systemic reactions to subcutaneous immunotherapy occur despite all necessary precautions and experienced staff and should prompt a search for causative factors. We present an analysis of 11 reactions, 8 of them within a short period. The patients and reactions were evaluated regarding extract errors (composition, concentration), dosing errors, ignored contraindications to specific subcutaneous immunotherapy, introductions versus maintenance phase and accidental intravascular injection. No single or common cause could be identified. Statistical analysis suggests that exceptional clusters of systemic reactions such as these may be just random cumulations without identifiable cause. application/pdf https://sonar.ch/global/documents/5016 eng info:eu-repo/semantics/altIdentifier/doi/10.1159/000328753 info:eu-repo/semantics/altIdentifier/pmid/22056648 info:eu-repo/semantics/openAccess International archives of allergy and immunology. - 2012 Adolescent Adult Aged Allergens Desensitization, Immunologic Female Humans Hypersensitivity, Immediate Injections, Subcutaneous Logistic Models Male Middle Aged Plant Extracts Respiratory Hypersensitivity Retrospective Studies Vaccines Young Adult Analysis of an unusual cluster of systemic reactions to subcutaneous specific immunotherapy with respiratory allergens in one allergy center. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:5020 2025-10-24T20:14:32Z

Brand Y Setz C Levano S Listyo A Chavez E Pak K Sung M Radojevic V Ryan AF Bodmer D 2011 BACKGROUND Inhibitors of 3-hydroxy-3-methylglutaryl-coenzyme A reductase, known as statins, are commonly used as cholesterol-lowering drugs. During the past decade, evidence has emerged that statins also have neuroprotective effects. Research in the retina has shown that simvastatin, a commonly used statin, increases Akt phosphorylation in vivo, indicating that the PI3K/Akt pathway contributes to the protective effects achieved. While research about neuroprotective effects have been conducted in several systems, the effects of statins on the inner ear are largely unknown. RESULTS We evaluated whether the 3-hydroxy-3-methylglutaryl-coenzyme A reductase is present within the rat cochlea and whether simvastatin is able to protect auditory hair cells from gentamicin-induced apoptotic cell death in a in vitro mouse model. Furthermore, we evaluated whether simvastatin increases Akt phosphorylation in the organ of Corti. We detected 3-hydroxy-3-methylglutaryl-coenzyme A reductase mRNA in organ of Corti, spiral ganglion, and stria vascularis by reverse transcriptase-polymerase chain reaction (RT-PCR). Moreover, we observed a dose-dependent and significant reduction of hair cell loss in organs of Corti treated with simvastatin in addition to gentamicin, as compared to samples treated with gentamicin alone. The protective effect of simvastatin was reversed by addition of mevalonate, a downstream metabolite blocked by simvastatin, demonstrating the specificity of protection. Finally, Western blotting showed an increase in organ of Corti Akt phosphorylation after simvastatin treatment in vitro. CONCLUSION These results suggest a neuroprotective effect of statins in the inner ear, mediated by reduced 3-hydroxy-3-methylglutaryl-coenzyme A reductase metabolism and Akt activation. https://sonar.ch/global/documents/5020 eng info:eu-repo/semantics/altIdentifier/doi/10.1186/1471-2202-12-114 info:eu-repo/semantics/altIdentifier/pmid/22082490 BMC neuroscience. - 2011 Animals Animals, Newborn Disease Models, Animal Gentamicins Hair Cells, Auditory Hearing Loss, Sensorineural Hydroxymethylglutaryl-CoA Reductase Inhibitors Mice Mice, Transgenic Nerve Degeneration Neuroprotective Agents Primary Cell Culture Proto-Oncogene Proteins c-akt Rats Rats, Wistar Signal Transduction Simvastatin Simvastatin protects auditory hair cells from gentamicin-induced toxicity and activates Akt signaling in vitro. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:5038 2025-10-24T20:14:34Z

Kind F Scherer K Bircher AJ 2012 BACKGROUND The increased frequency of case reports of allergic contact dermatitis from non-permanent black henna tattoos in recent years shows the popularity of this form of body painting. PATIENTS AND METHODS Seven patients presented with allergic contact dermatitis after initial hair or eyelash dyeing. They all had a history of a previous reaction from a black henna tattoo. All were patch tested with the European standard patch test series and the standard supplemental series, as well as special series for dyes and hairdressers. RESULTS All seven patients showed a positive reaction in patch testing with para-phenylenediamine (PPD) (0.3 % and/or 1.0 % in pet.). Five patients also had positive reactions to other dyes such as aminophenol, para-toluene diamine, disperse orange and yellow and four patients reacted to benzocaine. These were interpreted as cross-reactions. The time from sensitization by the black henna tattoo to the onset of allergic contact dermatitis after hair dyeing was an average of 6.2 years. CONCLUSIONS The most common cause of allergic contact dermatitis after black henna tattoos is PPD. Both the long skin contact and the high concentrations of PPD increase the risk of sensitization. Allergic contact dermatitis may be followed by post-inflammatory hyper- or hypopigmentation, scarring and lifelong sensitization, which can have occupational impact, especially for hair dressers and cosmeticians. https://sonar.ch/global/documents/5038 eng info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1610-0387.2011.07882.x info:eu-repo/semantics/altIdentifier/pmid/22299643 Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG. - 2012 Adolescent Adult Dermatitis, Allergic Contact Female Hair Dyes Humans Male Middle Aged Naphthoquinones Phenylenediamines Tattooing Contact dermatitis to para-phenylenediamine in hair dye following sensitization to black henna tattoos - an ongoing problem. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:5068 2025-10-24T20:14:37Z

Broz P Jaeger KA 2012 https://sonar.ch/global/documents/5068 eng info:eu-repo/semantics/altIdentifier/doi/10.1177/1358863X12442265 info:eu-repo/semantics/altIdentifier/pmid/22556152 Vascular medicine (London, England). - 2012 Fingers Gangrene Humans Male Marijuana Smoking Middle Aged Risk Factors Smoking Thromboangiitis Obliterans Treatment Outcome Vasodilator Agents Wound Healing Images in vascular medicine. Buerger's disease. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:5069 2025-10-24T20:14:37Z

Hynes NE Smirnova T 2012 Ling and colleagues demonstrated that loss of the conditional 14-3-3σ allele results in accelerated HER2/ERBB2-driven mammary tumorigenesis and metastasis. This study underscores the role of 14-3-3σ as a potent tumor suppressor in ERBB2-driven tumor initiation and progression. application/pdf https://sonar.ch/global/documents/5069 eng info:eu-repo/semantics/altIdentifier/doi/10.1158/2159-8290.CD-11-0317 info:eu-repo/semantics/altIdentifier/pmid/22585164 info:eu-repo/semantics/openAccess Cancer discovery. - 2012 14-3-3 Proteins Animals Female Mammary Neoplasms, Experimental Receptor, ErbB-2 The 14-3-3σ tumor suppressor has multiple functions in ErbB2-induced breast cancer. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:5026 2025-10-24T20:14:40Z

Gaillard RC Abeywickrama K Brownell J Muller AF 1989 CV 205-502, an octahydrobenzo[g]quinoline, is a dopamine agonist compound that is not an ergot or ergoline derivative. To investigate the site of action of CV 205-502, three groups of five men were given single daily doses of CV 205-502 (0.04, 0.06, or 0.08 mg/day, doses that suppress plasma PRL by 60-80% for 24 h) for 5 days; on day 6 a combined anterior pituitary function test using iv administration of four hypothalamic releasing hormones (TRH, 200 micrograms; GHRH, 100 micrograms; CRH, 100 micrograms; LHRH, 100 micrograms) was performed. One month later the challenge tests were repeated to obtain control values. The following hormones were measured by RIA in plasma: TSH, ACTH, cortisol, PRL, GH, LH, FSH, and testosterone. With the exception of plasma PRL levels, basal and releasing hormone-stimulated values were similar after CV 205-502 administration and after the 1-month washout period. Basal plasma PRL was lower after CV 205-502 administration, and the response to TRH was attenuated by all three doses of CV 205-502 (the mean percent inhibition values were 76%, 93%, and 94%, respectively). All three doses of CV 205-502 were well tolerated, and another group of men well tolerated 0.1 mg daily. The results confirm that CV 205-502 is a potent dopamine agonist, which directly inhibits lactotropic cells but has no effect on other pituitary cell types. https://sonar.ch/global/documents/5026 eng info:eu-repo/semantics/altIdentifier/doi/10.1210/jcem-68-2-329 info:eu-repo/semantics/altIdentifier/pmid/2563733 The Journal of clinical endocrinology and metabolism. - 1989 Adult Aminoquinolines Binding Sites Corticotropin-Releasing Hormone Dopamine Agents Dose-Response Relationship, Drug Gonadotropin-Releasing Hormone Growth Hormone-Releasing Hormone Humans Hypothalamus Male Pituitary Function Tests Pituitary Gland, Anterior Pituitary Hormones, Anterior Prolactin Receptors, Dopamine Testosterone Thyrotropin-Releasing Hormone Specific effect of CV 205-502, a potent nonergot dopamine agonist, during a combined anterior pituitary function test. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:5059 2025-10-24T20:14:45Z

Golaz O Sanchez JC James RW Hochstrasser DF 1995 Apolipoprotein E (apo E) is a normal component of several classes of plasma lipoproteins. Apo E phenotypes are closely related to total cholesterol, low density lipoprotein (LDL)-cholesterol and apo B concentration. The apo E 2/2 phenotype is related to the type III hyperlipoproteinemia due to the defective binding of apo E-2 to the hepatic receptors. The apo E 4/4 phenotype has been reported to be present in most elderly people suffering from the Alzheimer disease, and is associated with increased risk of coronary heart disease and Creutzfeld-Jakob disease. Therefore, apo E phenotyping is essential. The method described here uses a precast immobilized pH gradient, avoids time-consuming separation of lipoproteins from plasma, needs no pretreatment with neuraminidase and involves highly sensitive enhanced chemiluminescence for visualization. Therefore it has many advantages over previously published methods. https://sonar.ch/global/documents/5059 eng info:eu-repo/semantics/altIdentifier/doi/10.1002/elps.11501601196 info:eu-repo/semantics/altIdentifier/pmid/7498163 Electrophoresis. - 1995 Apolipoproteins E Electrophoresis, Gel, Two-Dimensional Electrophoresis, Polyacrylamide Gel Humans Hydrogen-Ion Concentration Phenotype Phenotyping of apolipoprotein E using immobilized pH gradient gels for one-dimensional and two-dimensional separations. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:5072 2025-10-24T20:14:49Z

Imbach P 2012 Since 1946 the development of fractionation and purification methods of human plasma led to biologic immunoglobulin for intravenous use (IVIG). In 1980 a child with refractory immune thrombocytopenia (ITP), bleeding and secondary hypogammaglobulinaemia due to long term immunosuppressive treatment got IVIG. His platelet counts dramatically increased. In 13 consecutive children with ITP, but without hypogammaglobulinaemia, similar rapid platelet increases were observed and confirmed in a controlled, multicentre study. During the past three decades this biologic treatment modality evoked clinical and laboratory research on the mechanisms of action and in disorders with similar immune pathogenesis. It was recognised that IVIG modulates the disturbed immune response in multiple, synergistic ways between the different components of the immune system. Beside other immune hematologic disorders other inflammatory and autoimmune diseases, mainly in the field of neurology and dermatology, IVIG showed beneficial effects. The worldwide consumption of IVIG increased from 300 kg per year in 1980 to 100 tonnes per year in 2010. Due to the heterogeneity of immunopathological mechanisms of autoimmune diseases evidence based indications of IVIG remain rare and off label use high. Registries of large numbers of patients and first endpoints of defining less heterogenous subgroups in immune related disorders are the next steps toward establishment of evidence based IVIG indications. https://sonar.ch/global/documents/5072 eng info:eu-repo/semantics/altIdentifier/doi/10.4414/smw.2012.13593 info:eu-repo/semantics/altIdentifier/pmid/22653800 Swiss medical weekly. - 2012 Autoimmune Diseases History, 20th Century Humans Immunoglobulins, Intravenous Purpura, Thrombocytopenic, Idiopathic Treatment of immune thrombocytopenia with intravenous immunoglobulin and insights for other diseases. A historical review. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:5090 2025-10-24T20:14:52Z

Kantengwa S Müller I Louis J Polla BS 1995 Heat shock/stress proteins (HSP) represent the most conserved proteins expressed in prokaryotes and eukaryotes. These constitutive and inducible proteins function as molecular chaperones and are part of virulence factors. They participate in self/non-self discrimination and may protect phagocytes from the toxic effects of the reactive oxygen species generated by these cells during bacterial phagocytosis and infection. In this study, we investigated the early stress response of host cells [either human alveolar macrophages (AM) or murine peritoneal macrophages (PM)] during infection by an obligate intracellular parasite (Leishmania major), which lives within phagolysosomes. Immunoblotting with specific antibodies demonstrated that L. major had no effect on host stress protein synthesis, but synthesized high levels of its own stress proteins within AM and PM. The lack of induction of a host cell stress response may relate to the failure of L. major to activate the respiratory burst in these cells, whereas the upshift of L. major HSP within macrophages is part of an adaptive response of the parasite to the host. https://sonar.ch/global/documents/5090 eng info:eu-repo/semantics/altIdentifier/doi/10.1038/icb.1995.12 info:eu-repo/semantics/altIdentifier/pmid/7768547 Immunology and cell biology. - 1995 Animals Cells, Cultured Female Heat-Shock Proteins Humans Interferon-gamma Leishmania major Macrophages Mice Mice, Inbred BALB C Mice, Inbred DBA Protozoan Proteins Superoxides Infection of human and murine macrophages with Leishmania major is associated with early parasite heat shock protein synthesis but fails to induce a host cell stress response. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:5093 2025-10-24T20:14:52Z

Marti MC Roche B Gumener RG 1994 https://sonar.ch/global/documents/5093 eng info:eu-repo/semantics/altIdentifier/doi/10.1007/bf00290195 info:eu-repo/semantics/altIdentifier/pmid/7814992 International journal of colorectal disease. - 1994 Humans Perineum Surgical Flaps Treatment Outcome Skin cover of perineal defects using V-Y flaps. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:5105 2025-10-24T20:14:55Z

Kahlen JP Carlberg C 1994 Calcitriol (1,25-dihydroxyvitamin D3, VD) controls multiple aspects of homeostasis, cell growth and differentiation by the action of its nuclear receptor (VDR), which binds to, and activates transcription from, response elements in the promoter region of its target genes. One of these target genes is calcitriol 24-hydroxylase, an enzyme that initiates the degradation of 25-dihydroxyvitamin D3 (calcidiol) and calcitriol. We screened the promoter of rat calcitriol 24-hydroxylase for potential VDR binding sites and identified a functional VD response element, between positions -250 and -233. This response element consists of two directly repeated hexameric core binding motifs spaced by six nucleotides and confers VD-dependent transactivation mediated by VDR homodimers or alternatively by heterodimers formed by VDR and retinoic acid receptor (RAR). Its structure and function are very similar to those of the homodimer-type response element of the human osteocalcin promoter. https://sonar.ch/global/documents/5105 eng info:eu-repo/semantics/altIdentifier/doi/10.1006/bbrc.1994.2081 info:eu-repo/semantics/altIdentifier/pmid/8060315 Biochemical and biophysical research communications. - 1994 Animals Base Sequence Binding Sites Cells, Cultured Cytochrome P-450 Enzyme System Drosophila Molecular Sequence Data Oligodeoxyribonucleotides Promoter Regions, Genetic Rats Receptors, Calcitriol Sequence Alignment Steroid Hydroxylases Tumor Cells, Cultured Vitamin D3 24-Hydroxylase Identification of a vitamin D receptor homodimer-type response element in the rat calcitriol 24-hydroxylase gene promoter. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:5116 2025-10-24T20:14:56Z

Brosi R Gröning K Behrens SE Lührmann R Krämer A 1993 In the assembly of a prespliceosome, U2 small nuclear ribonucleoprotein (snRNP) functions in pre-messenger RNA (mRNA) splicing together with splicing factors (SFs) 3a, SF3b, and several other proteins. The 17S but not the 12S form of U2 snRNP is active in splicing-complex formation. Here it is shown that the SF3a subunits correspond to three of the 17S U2 snRNP-specific polypeptides. SF3a interacts with U2 snRNP in the presence of SF3b to generate a structure similar to 17S U2 snRNP, which suggests a function for SF3a and SF3b in the incorporation of U2 snRNP into the spliceosome. Furthermore, the 60-kilodalton subunit of SF3a is related to the yeast splicing protein PRP9. https://sonar.ch/global/documents/5116 eng info:eu-repo/semantics/altIdentifier/doi/10.1126/science.8211112 info:eu-repo/semantics/altIdentifier/pmid/8211112 Science (New York, N.Y.). - 1993 Adenosine Triphosphate Cross Reactions Electrophoresis, Polyacrylamide Gel Fungal Proteins Humans Nuclear Proteins RNA Splicing RNA Splicing Factors RNA-Binding Proteins Ribonucleoprotein, U2 Small Nuclear Saccharomyces cerevisiae Proteins Spliceosomes Interaction of mammalian splicing factor SF3a with U2 snRNP and relation of its 60-kD subunit to yeast PRP9. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:5137 2025-10-24T20:14:59Z

Kiss JZ Wang C Rougon G 1993 The adult hypothalamo-neurohypophysial system of the rat retains the capacity to express highly polysialylated isoforms of neural cell adhesion molecule normally expressed in developing tissues. Here we report that the expression of these isoforms in neurohypophysial astrocytes (pituicytes) may be regulated by neurosecretory cells. In the intact neurohypophysis a strong and homogeneously distributed immunostaining for the "embryonic", highly sialylated form of neural cell adhesion molecules was detected by light-microscopic immunocytochemistry. By electron-microscopy, both neurosecretory axons and pituicytes were immunoreactive for this isoform. However, in contrast to the rather uniform staining on nerve fibres, polysialic acid immunolabelling on glial surfaces was uneven: immunostaining could be observed on glial surfaces facing neuronal elements, but not at contact sites between pituicytes. In addition, most glial and neuronal elements were heavily and evenly labelled with the polyclonal antibody recognizing "total" neural cell adhesion molecule. Surgical transection of the hypophysial stalk, a procedure that eliminates descending neurosecretory axons from the neurohypophysis, resulted in the complete disappearance of polysialic immunoreactivity from the neurohypophysis. The electron-microscopic analysis confirmed that cell surfaces of pituicytes lacked this immunoreactivity after the lesion. When residual neurosecretory axons were observed following an incomplete lesion, immunoreactivity on axons and glial processes was maintained. Transection did not affect the distribution of "total" neural cell adhesion molecule. We postulate that the presence of neurosecretory axons in the neurohypophysis is necessary to maintain the capacity of pituicytes to express immunoreactivity for the polysialylated isoforms of neural cell adhesion molecule but not the neural cell adhesion molecule itself since immunoreactivity for "total" neural cell adhesion molecule was unaltered after hypophysial stalk transection.(ABSTRACT TRUNCATED AT 250 WORDS) https://sonar.ch/global/documents/5137 eng info:eu-repo/semantics/altIdentifier/doi/10.1016/0306-4522(93)90299-u info:eu-repo/semantics/altIdentifier/pmid/8469308 Neuroscience. - 1993 Animals Astrocytes Axons Cell Adhesion Molecules, Neuronal Immunohistochemistry Male Microscopy, Immunoelectron Neuronal Plasticity Pituitary Gland Pituitary Gland, Posterior Rats Rats, Sprague-Dawley Sialic Acids Nerve-dependent expression of high polysialic acid neural cell adhesion molecule in neurohypophysial astrocytes of adult rats. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:5144 2025-10-24T20:15:01Z

Galetto-Lacour A Yerly S Perneger TV Baumberger C Hirschel B Perrin L 1996 Human immunodeficiency virus (HIV) viremia was evaluated in 73 patients with long-standing infection to investigate its relationship with clinical or biologic parameters and to assess its use as a predictor of clinical progression and death. After adjustment for other parameters, baseline HIV RNA level was significantly associated with baseline clinical stage and CD4 cell count. During follow-up (mean, 14.6 months), 16 patients died; 34 others had clinical progression of disease. In multivariate analysis, mortality was better predicted by baseline CD4 cell count (relative hazard [RH] for 100-cell decrease, 3.5; 95% confidence interval [CI], 1.5-8.2; P = .003) than by HIV RNA (P = .28) or clinical stage. HIV RNA level was the best predictor of clinical progression (RH for 1 log increase, 2.8; 95% CI, 1.6-4.9; P < .001). Monitoring of HIV RNA level may help to identify patients who might benefit from antiretroviral or prophylactic therapy. application/pdf https://sonar.ch/global/documents/5144 eng info:eu-repo/semantics/altIdentifier/doi/10.1093/infdis/173.6.1388 info:eu-repo/semantics/altIdentifier/pmid/8648210 info:eu-repo/semantics/openAccess The Journal of infectious diseases. - 1996 Adult CD4 Lymphocyte Count Cohort Studies Disease Progression Disease-Free Survival Female HIV HIV Infections Humans Male Middle Aged Predictive Value of Tests Prognosis Prospective Studies RNA, Viral Viremia Prognostic value of viremia in patients with long-standing human immunodeficiency virus infection. Swiss HIV Cohort Study Group. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:5159 2025-10-24T20:15:02Z

Golaz O Wilkins MR Sanchez JC Appel RD Hochstrasser DF Williams KL 1996 Expression of different genomes can be studied by high-resolution two-dimensional electrophoresis (2-D PAGE). To help these studies, two-dimensional reference maps of different biological tissues and fluids have been built and can be found in the SWISS-2DPAGE database, accessible via the World Wide Web network on the ExPASy molecular biology server. Different techniques were used to identify the polypeptides. At the present time, the method considered to be the fastest and the most cost-effective is amino acid composition analysis (AAC). Proteins, transferred onto polyvinylidene (PVDF) membranes, were submitted to vapor-phase hydrolysis, derivatized with 9-fluorenylmethyl chloroformate (FMOC) and separated on an ODS-Hypersil column. Identification was obtained by using the program 'AACompIdent' available from ExPASy. In this work, different experimental parameters, such as contamination, reproducibility and accuracy, have been assessed. First, it has been found that a major source of contamination was human keratin. Next, amino acids have been classified into 'reliable' and 'nonreliable'. Accordingly, 'bias' and 'weights' were defined for each amino acid, which could be set in the 'AACompIdent' program. Finally, examples of identification, including the use of Edman degradation sequence tagging, are described. https://sonar.ch/global/documents/5159 eng info:eu-repo/semantics/altIdentifier/doi/10.1002/elps.1150170328 info:eu-repo/semantics/altIdentifier/pmid/8740182 Electrophoresis. - 1996 Amino Acid Sequence Databases, Factual Electrophoresis, Gel, Two-Dimensional Evaluation Studies as Topic Hydrolysis Molecular Sequence Data Proteins Reproducibility of Results Software Identification of proteins by their amino acid composition: an evaluation of the method. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:5125 2025-10-24T20:15:04Z

François P Vaudaux P Lew PD 1998 Foreign body implants are highly susceptible to microorganism infection. The infectious agents may be of low pathogenicity (such as S. epidermidis) or involve more virulent strains (such as S. aureus). The common denominator for the three main elements that play a role in the physiopathology of such infections (bacteria, neutrophils, and different biomaterials) are host proteins deposited over the surface of the devices immediately after their implantation. These proteins modulate that host cells response but can also promote Staphylococcus adhesion to the biomaterial. Neutrophils and other cells such as fibroblasts adhere to several extracellular matrix proteins such as fibronectin, fibrinogen, collagen, vitronectin, via specific cell surface receptor. The evolution of the technology and the increasing numbers of long-term artificial implants require a better understanding of fundamental mechanisms of foreign body infections to reduce their incidence and optimize their treatment. https://sonar.ch/global/documents/5125 eng info:eu-repo/semantics/altIdentifier/doi/10.1007/s100169900112 info:eu-repo/semantics/altIdentifier/pmid/9451994 Annals of vascular surgery. - 1998 Bacterial Adhesion Blood Proteins Catheterization, Central Venous Extracellular Matrix Proteins Foreign-Body Reaction Humans Neutrophils Staphylococcal Infections Role of plasma and extracellular matrix proteins in the physiopathology of foreign body infections. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:5148 2025-10-24T20:15:06Z

Allaz AF Vannotti M Desmeules J Piguet V Celik Y Pyroth O Guez P Dayer P 1998 The use of the term "litigation neurosis"--a condition with controversial clinical significance--might correspond to the expression for a difficult physician-patient relationship. The characteristics of patients with a DSM-III-R diagnosis of somatoform pain disorder who had been labeled "litigation neurosis" by their physicians were explored. Among 74 patients referred to a pain clinic, 30% had been labeled litigation neurosis, and among 81 patients referred while claiming disability benefits, 19% had been thus categorized. The attribution of this label was neither correlated to actually being involved in a claim for disability benefits nor to involvement in legal action. Patients who had been designated with litigation neurosis were characterized by a lower educational level, a higher rate of DSM-III-R major depression, and a much higher frequency of personality disorders than patients who were not thus labeled. We postulate that the communication style of patients with this constellation of characteristics, in particular the presence of psychiatric comorbidity, may have engendered a difficult doctor-patient relationship, leading physicians to use the label in the absence of objective evidence of litigation or involvement in legal action. We agree that the inappropriate use of labels such as "litigation neurosis" should be questioned. https://sonar.ch/global/documents/5148 eng info:eu-repo/semantics/altIdentifier/doi/10.1016/s0163-8343(97)00127-8 info:eu-repo/semantics/altIdentifier/pmid/9582593 General hospital psychiatry. - 1998 Adult Attitude of Health Personnel Chi-Square Distribution Chronic Disease Comorbidity Disability Evaluation Female Humans Male Middle Aged Neurotic Disorders Pain Personality Disorders Physician-Patient Relations Prevalence Socioeconomic Factors Somatoform Disorders Switzerland Terminology as Topic Use of the label "litigation neurosis" in patients with somatoform pain disorder. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:5197 2025-10-24T20:15:12Z

Fabre-Jonca N Viard I French LE Masson D 1999 Microtubules are involved in the positioning and movement of organelles and vesicles and therefore play fundamental roles in cell polarization and differentiation. Their organization and properties are cell-type specific and are controlled by microtubule-associated proteins (MAP). E-MAP-115 (epithelial microtubule-associated protein of 115 kDa) has been identified as a microtubule-stabilizing protein predominantly expressed in epithelial cells. We have used human skin and primary keratinocytes as a model to assess a putative function of E-MAP-115 in stabilizing and reorganizing the microtubule network during epithelial cell differentiation. Immunolabeling of skin sections indicated that E-MAP-115 is predominantly expressed in the suprabasal layers of the normal epidermis and, in agreement with this observation, is relatively abundant in squamous cell carcinomas but barely detectable in basal cell carcinomas. In primary keratinocytes whose terminal differentiation was induced by increasing the Ca2+ concentration of the medium, E-MAP-115 expression significantly increased during the first day, as observed by northern and western blot analysis. Parallel immunofluorescence studies showed an early redistribution of E-MAP-115 from microtubules with a paranuclear localization to cortical microtubules organized in spike-like bundles facing intercellular contacts. This phenomenon is transient and can be reversed by Ca2+ depletion. Treatment of cells with cytoskeleton-active drugs after raising the Ca2+ concentration indicated that E-MAP-115 is associated with a subset of stable microtubules and that the cortical localization of these microtubules is dependent on other microtubules but not on strong interactions with the actin cytoskeleton or the plasma membrane. The mechanism whereby E-MAP-115 would redistribute to and stabilize cortical microtubules used for the polarized transport of vesicles towards the plasma membrane, where important reorganizations take place upon stratification, is discussed. https://sonar.ch/global/documents/5197 eng info:eu-repo/semantics/altIdentifier/doi/10.1046/j.1523-1747.1999.00500.x info:eu-repo/semantics/altIdentifier/pmid/9989799 The Journal of investigative dermatology. - 1999 Cytochalasin D Humans Intracellular Fluid Keratinocytes Microtubule-Associated Proteins Skin Neoplasms Up-Regulation Upregulation and redistribution of E-MAP-115 (epithelial microtubule-associated protein of 115 kDa) in terminally differentiating keratinocytes is coincident with the formation of intercellular contacts. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:5208 2025-10-24T20:15:13Z

Par M Spanovic N Tauböck TT Attin T Tarle Z 2019 Resin composites containing reinforcing inert glass fillers combined with bioactive glass (BG) can aid in the prevention of secondary caries, which is a major cause of failure of contemporary composite restorations. A series of previous studies on experimental resin composites filled with BG 45S5 has demonstrated that methacrylate resin polymerization can be impaired by the addition of unsilanized BG, leading to lower degrees of conversion (DC). In order to distinguish whether the polymerization inhibition is caused by a direct (temperature-independent) effect of BG or an indirect (temperature-dependent) effect of restricted mobility of reactive species, this study used Raman spectroscopy to evaluate the DC values of experimental composites post-cured at 37 °C and 150 °C. The potential of BG to adversely affect DC was highly dependent on the resin system. The highest DC reduction was observed in the resin system based on ethoxylated bisphenol A dimethacrylate (Bis-EMA), followed by bisphenol A glycidyl methacrylate (Bis-GMA). In contrast, the DC for urethane dimethacrylate (UDMA) was not compromised by BG. Increasing the mobility of reactive species by heating at 150 °C showed limited potential for increasing the DC in the Bis-EMA and Bis-GMA resin systems, indicating a direct inhibitory effect of BG on polymerization. application/pdf https://sonar.ch/global/documents/5208 eng info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-019-54035-y info:eu-repo/semantics/altIdentifier/pmid/31754180 info:eu-repo/semantics/openAccess Scientific reports. - 2019 Multidisciplinary Degree of conversion of experimental resin composites containing bioactive glass 45S5: the effect of post-cure heating. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:5182 2025-10-24T20:15:20Z

Baeyens V Varesio E Veuthey JL Gurny R 1997 A selective capillary zone electrophoresis (CZE) microassay was developed for the simultaneous determination of dexamethasone phosphate and its major metabolite, dexamethasone, in tears. The calibration was carried out in the biological matrix with indoprofen as an internal standard which allowed the separation of dexamethasone phosphate and dexamethasone from the tear constituents. The limits of detection and quantification of the assay were 0.5 and 2.0 microg ml(-1), respectively. This quantification method is essential for the in vivo determination of dexamethasone concentration-time profiles in tears after application of the antiinflammatory drug. https://sonar.ch/global/documents/5182 eng info:eu-repo/semantics/altIdentifier/doi/10.1016/s0378-4347(96)00494-x info:eu-repo/semantics/altIdentifier/pmid/9187404 Journal of chromatography. B, Biomedical sciences and applications. - 1997 Administration, Topical Anti-Inflammatory Agents Dexamethasone Electrophoresis, Capillary Glucocorticoids Sensitivity and Specificity Tears Determination of dexamethasone in tears by capillary electrophoresis. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:5219 2025-10-24T20:15:23Z

Barazzone Argiroffo C Donati YR Boccard J Rochat AF Vesin C Kan CD Piguet PF 2002 Recent studies suggest that apoptosis plays a role in oxygen-induced injury, although the activation pathways and the executioner proteases that lead to cleavage of lung cell proteins and DNA, are not yet identified. We explored previously the tumor necrosis factor/tumor necrosis factor receptor and the Fas/FasL, belonging to the intrinsic pathway, and could not demonstrate any protective effect by interfering with these cell receptors. Lately, it has been shown that interacting with the CD40 system, also known to promote cell death, by administering anti-CD40 ligand (L) antibody was beneficial in several diseases and, in particular, in hyperoxia-induced injury. Using CD40- and CD40L-deficient mice (-/-) as well as administering anti-CD40L antibody, we examined the extent of lung injury in oxygen-breathing mice by several ways (lung weight, histology, inflammatory mediators, and DNA ladder) as well as the mortality. The development of lung injury was similar in wild-type, CD40-/-, CD40L-/-, or in wild-type mice treated with anti-CD40L antibody. Apoptosis was present in all conditions at 72 hours of oxygen exposure. These results show that oxygen-induced injury does not require CD40-CD40L interaction and that apoptosis of lung cells does not involve this pathway. application/pdf https://sonar.ch/global/documents/5219 eng info:eu-repo/semantics/altIdentifier/doi/10.1016/S0002-9440(10)64350-7 info:eu-repo/semantics/altIdentifier/pmid/11786400 info:eu-repo/semantics/openAccess The American journal of pathology. - 2002 Animals Antibodies Apoptosis Bronchoalveolar Lavage Fluid CD40 Antigens CD40 Ligand Cytokines Hyperoxia Lung Mice Mice, Inbred C57BL Mice, Knockout RNA, Messenger Time Factors CD40-CD40 ligand disruption does not prevent hyperoxia-induced injury. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:5259 2025-10-24T20:15:26Z

Loubeyre P Debard I Nemoz C Minh VA 2002 OBJECTIVE The main goal of this study was to determine whether in thoracic helical CT for general indications a high opacification of hilar pulmonary vessels could be obtained using a small amount of nonionic contrast medium. SUBJECTS AND METHODS Two hundred consecutive patients referred for contrast-enhanced thoracic CT for malignancies or infections prospectively entered the study. They were randomly assigned to one of two simple bolus injection protocols (100 patients in each protocol): 60 mL of a nonionic contrast agent (250 mg I/mL) injected at a 3 mL/sec flow rate, or 80 mL of the same contrast agent injected at a 4 mL/sec flow rate. No saline flush or bolus triggering system was used. Hilar and mediastinal vessel enhancement was qualitatively (using a 4-point scale) and quantitatively (arterial attenuation values) assessed. Perivenous artifacts were also assessed. RESULTS No extensive perivenous artifacts were noted. No significant difference was noted regarding pulmonary venous enhancement. Excellent opacification of the pulmonary veins was observed in 66% of patients injected at 3 mL/sec and in 56% of patients injected at 4 mL/sec (p > 0.192). A highly significant difference was noted for pulmonary artery enhancement. Excellent opacification of the pulmonary arteries was noted in 83% of patients injected with a 3mL/sec flow rate and in 61% of patients injected with a 4 mL/sec flow rate (p = 0.001). CONCLUSION A high level of opacification of hilar pulmonary vessels, with no major perivenous artifacts, can be obtained with a small amount of nonionic contrast medium using a simple bolus injection. https://sonar.ch/global/documents/5259 eng info:eu-repo/semantics/altIdentifier/doi/10.2214/ajr.178.6.1781377 info:eu-repo/semantics/altIdentifier/pmid/12034600 AJR. American journal of roentgenology. - 2002 Adult Aged Aged, 80 and over Contrast Media Female Humans Male Middle Aged Prospective Studies Radiography, Thoracic Tomography, X-Ray Computed High opacification of hilar pulmonary vessels with a small amount of nonionic contrast medium for general thoracic CT: a prospective study. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:5237 2025-10-24T20:15:28Z

Bernasconi L Şen S Angerame L Balyegisawa AP Hong Yew Hui D Hotter M Hsu CY Ito T Jörger F Krassnitzer W Phillips AT Li R Stockley L Tay F von Heijne Widlund C Wan M Wong C Yau H Hiemstra TF Uresin Y Senti G 2020 BACKGROUND The progress of electronic health technologies and biobanks holds enormous promise for efficient research. Evidence shows that studies based on sharing and secondary use of data/samples have the potential to significantly advance medical knowledge. However, sharing of such resources for international collaboration is hampered by the lack of clarity about ethical and legal requirements for transfer of data and samples across international borders. MAIN TEXT Here, the International Clinical Trial Center Network (ICN) reports the legal and ethical requirements governing data and sample exchange (DSE) across four continents. The most recurring requirement is ethical approval, whereas only in specific conditions approval of national health authorities is required. Informed consent is not required in all sharing situations. However, waiver of informed consent is only allowed in certain countries/regions and under certain circumstances. The current legal and ethical landscape appears to be very complex and under constant evolution. Regulations differ between countries/regions and are often incomplete, leading to uncertainty. CONCLUSION With this work, ICN illuminates the unmet need for a single international collaborative framework to facilitate DSE. Harmonising requirements for global DSE will reduce inefficiency and waste in research. There are many challenges to realising this ambitious vision, including inconsistent terminology and definitions, and heterogeneous and dynamic legal constraints. Here, we identify areas of agreement and significant difference as a necessary first step towards facilitating international collaboration. We propose the establishment of a working group to continue the comparison across jurisdictions, create a standardised glossary and define a set of basic principles and fundamental requirements for DSE. https://sonar.ch/global/documents/5237 eng info:eu-repo/semantics/altIdentifier/doi/10.1186/s12910-020-0448-9 info:eu-repo/semantics/altIdentifier/pmid/31964390 BMC medical ethics. - 2020 Big data Biobanking Data sharing International exchange Research policies Electronic Health Records Global Health Humans Information Dissemination International Cooperation Internationality Ownership Tissue Banks Legal and ethical framework for global health information and biospecimen exchange - an international perspective. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:5238 2025-10-24T20:15:28Z

Savarino E di Pietro M Bredenoord AJ Carlson DA Clarke JO Khan A Vela MF Yadlapati R Pohl D Pandolfino JE Roman S Gyawali CP 2020 The functional lumen imaging probe (FLIP) measures luminal dimensions using impedance planimetry, performed most often during sedated upper endoscopy. Mechanical properties of the esophageal wall and opening dynamics of the esophagogastric junction (EGJ) can be objectively evaluated in esophageal motor disorders, eosinophilic esophagitis, esophageal strictures, during esophageal surgery and in postsurgical symptomatic states. Distensibility index, the ratio of EGJ cross sectional area to intraballoon pressure, is the most useful FLIP metric. Secondary peristalsis from balloon distension can be displayed topographically as repetitive anterograde or retrograde contractile activity in the esophageal body, similar to high-resolution manometry. Real-time interpretation and postprocessing of FLIP metadata can complement the identification of esophageal outflow obstruction and achalasia, especially when findings are inconclusive from alternate esophageal tests in symptomatic patients. FLIP can complement the diagnosis of achalasia when manometry and barium studies are inconclusive or negative in patients with typical symptoms. FLIP can direct adequacy of disruption of the EGJ in achalasia when used during and immediately after myotomy and pneumatic dilation. Lumen diameter measured using FLIP in eosinophilic esophagitis and in complex strictures can potentially guide management. An abbreviated modification of the Grading of Recommendations Assessment, Development, and Evaluation was used to determine the quality of available evidence and recommendations regarding FLIP utilization. FLIP metrics that are diagnostic or suggestive of an abnormal motor pattern and metrics that define normal esophageal physiology were developed by consensus and are described in this review. https://sonar.ch/global/documents/5238 eng info:eu-repo/semantics/altIdentifier/doi/10.14309/ajg.0000000000000773 info:eu-repo/semantics/altIdentifier/pmid/33156096 The American journal of gastroenterology. - 2020 Hepatology Gastroenterology Use of the Functional Lumen Imaging Probe in Clinical Esophagology. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12314 2025-10-24T20:47:18Z

Galli A Robay D Osterwalder M Bao X Bénazet JD Tariq M Paro R Mackem S Zeller R 2010 The polarization of nascent embryonic fields and the endowment of cells with organizer properties are key to initiation of vertebrate organogenesis. One such event is antero-posterior (AP) polarization of early limb buds and activation of morphogenetic Sonic Hedgehog (SHH) signaling in the posterior mesenchyme, which in turn promotes outgrowth and specifies the pentadactylous autopod. Inactivation of the Hand2 transcriptional regulator from the onset of mouse forelimb bud development disrupts establishment of posterior identity and Shh expression, which results in a skeletal phenotype identical to Shh deficient limb buds. In wild-type limb buds, Hand2 is part of the protein complexes containing Hoxd13, another essential regulator of Shh activation in limb buds. Chromatin immunoprecipitation shows that Hand2-containing chromatin complexes are bound to the far upstream cis-regulatory region (ZRS), which is specifically required for Shh expression in the limb bud. Cell-biochemical studies indicate that Hand2 and Hoxd13 can efficiently transactivate gene expression via the ZRS, while the Gli3 repressor isoform interferes with this positive transcriptional regulation. Indeed, analysis of mouse forelimb buds lacking both Hand2 and Gli3 reveals the complete absence of antero-posterior (AP) polarity along the entire proximo-distal axis and extreme digit polydactyly without AP identities. Our study uncovers essential components of the transcriptional machinery and key interactions that set-up limb bud asymmetry upstream of establishing the SHH signaling limb bud organizer. application/pdf https://sonar.ch/global/documents/12314 eng info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pgen.1000901 info:eu-repo/semantics/altIdentifier/pmid/20386744 info:eu-repo/semantics/openAccess PLoS genetics. - 2010 Animals Basic Helix-Loop-Helix Transcription Factors Body Patterning Chromatin Embryo, Mammalian Gene Expression Regulation, Developmental Hedgehog Proteins Homeodomain Proteins Limb Buds Mice Mice, Transgenic Mutation Transcription Factors Distinct roles of Hand2 in initiating polarity and posterior Shh expression during the onset of mouse limb bud development. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12371 2025-10-24T20:47:23Z

Eisenring M vom Berg J Kristiansen G Saller E Becher B 2010 The potent tumoricidal activity of interleukin 12 (IL-12) is thought to be mediated by the activation and polarization of natural killer (NK) cells and T helper type 1 (T(H)1) cells, respectively. By systematic analysis of the IL-12-induced immune response to subcutaneous melanoma (B16), we found that tumor suppression was mediated independently of T lymphocytes or NK cells. IL-12 initiated local antitumor immunity by stimulating a subset of NKp46(+) lymphoid tissue-inducer (LTi) cells dependent on the transcription factor RORγt. The presence of these NKp46(+) LTi cells induced upregulation of adhesion molecules in the tumor vasculature and resulted in more leukocyte invasion. Thus, this innate cell type is responsive to IL-12 and is a powerful mediator of tumor suppression. https://sonar.ch/global/documents/12371 eng info:eu-repo/semantics/altIdentifier/doi/10.1038/ni.1947 info:eu-repo/semantics/altIdentifier/pmid/20935648 Nature immunology. - 2010 Animals Cell Line, Tumor Interleukin-12 Lymphoid Tissue Melanoma Mice Mice, Inbred C57BL Mice, Knockout Natural Cytotoxicity Triggering Receptor 1 Nuclear Receptor Subfamily 1, Group F, Member 3 Tumor Cells, Cultured IL-12 initiates tumor rejection via lymphoid tissue-inducer cells bearing the natural cytotoxicity receptor NKp46. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12381 2025-10-24T20:47:26Z

Scherrer D Schmid S Körner C 2011 Strong topographic variation interacting with low stature alpine vegetation creates a multitude of micro-habitats poorly represented by common 2 m above the ground meteorological measurements (weather station data). However, the extent to which the actual habitat temperatures in alpine landscapes deviate from meteorological data at different spatial scales has rarely been quantified. In this study, we assessed thermal surface and soil conditions across topographically rich alpine landscapes by thermal imagery and miniature data loggers from regional (2-km(2)) to plot (1-m(2)) scale. The data were used to quantify the effects of spatial sampling resolution on current micro-habitat distributions and habitat loss due to climate warming scenarios. Soil temperatures showed substantial variation among slopes (2-3 K) dependent on slope exposure, within slopes (3-4 K) due to micro-topography and within 1-m(2) plots (1 K) as a result of plant cover effects. A reduction of spatial sampling resolution from 1 × 1 m to 100 × 100 m leads to an underestimation of current habitat diversity by 25% and predicts a six-times higher habitat loss in a 2-K warming scenario. Our results demonstrate that weather station data are unable to reflect the complex thermal patterns of aerodynamically decoupled alpine vegetation at the investigated scales. Thus, the use of interpolated weather station data to describe alpine life conditions without considering the micro-topographically induced thermal mosaic might lead to misinterpretation and inaccurate prediction. application/pdf https://sonar.ch/global/documents/12381 eng info:eu-repo/semantics/altIdentifier/doi/10.1007/s00484-010-0364-7 info:eu-repo/semantics/altIdentifier/pmid/20924613 info:eu-repo/semantics/openAccess International journal of biometeorology. - 2011 Altitude Animals Biodiversity Ecosystem Global Warming Meteorological Concepts Microclimate Plant Development Soil Species Specificity Temperature Elevational species shifts in a warmer climate are overestimated when based on weather station data. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12407 2025-10-24T20:47:28Z

Bentz D Steiner M Meinlschmidt G 2012 BACKGROUND Premenstrual dysphoric disorder (PMDD) and severe premenstrual syndrome (PMS) are common, yet often remain unrecognized and not adequately treated. One reason for this is the lack of a valid German screening instrument. The aim of the present study was to create a German version of the English "Premenstrual Symptoms Screening Tool (PSST)" and to verify its applicability. MATERIAL AND METHODS The German version of the PSST was created as "Screening-Instrument für prämenstruelle Symptome (SIPS)" and its reliability and validity determined based on data from 47 women with and without PMDD/severe PMS using Internet-based daily symptom ratings. RESULTS The retest reliability of the SIPS was r=0.69, and Cronbach's alpha was 0.924. As an indicator of the convergent validity of the SIPS, there were significant differences between women with and without PMDD/severe PMS as identified by the SIPS with regard to prospectively assessed premenstrual symptomatology (F[2,44]=4.52, p<0.001) and symptom change (F[2,44]=25.23, p<0.001). CONCLUSIONS The SIPS is reliable and valid and may help to improve the identification of women who require treatment for their premenstrual symptoms. https://sonar.ch/global/documents/12407 eng info:eu-repo/semantics/altIdentifier/doi/10.1007/s00115-010-3210-6 info:eu-repo/semantics/altIdentifier/pmid/21221519 Der Nervenarzt. - 2012 Adult Female Germany Humans Mass Screening Premenstrual Syndrome Psychiatric Status Rating Scales Psychometrics Reproducibility of Results Sensitivity and Specificity Surveys and Questionnaires Young Adult [SIPS--screening instrument for premenstrual symptoms. The German version of Premenstrual Symptoms Screening Tool to assess clinically relevant disturbances]. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12416 2025-10-24T20:47:30Z

Leventhal ME Denhaerynck K Brunner-La Rocca HP Burnand B Conca-Zeller A Bernasconi AT Mahrer-Imhof R Froelicher ES De Geest S 2011 PRINCIPLES International guidelines for heart failure (HF) care recommend the implementation of inter-professional disease management programmes. To date, no such programme has been tested in Switzerland. The aim of this randomised controlled trial (RCT) was to test the effect on hospitalisation, mortality and quality of life of an adult ambulatory disease management programme for patients with HF in Switzerland. METHODS Consecutive patients admitted to internal medicine in a Swiss university hospital were screened for decompensated HF. A total of 42 eligible patients were randomised to an intervention (n = 22) or usual care group (n = 20). Medical treatment was optimised and lifestyle recommendations were given to all patients. Intervention patients additionally received a home visit by a HF-nurse, followed by 17 telephone calls of decreasing frequency over 12 months, focusing on self-care. Calls from the HF nurse to primary care physicians communicated health concerns and identified goals of care. Data were collected at baseline, 3, 6, 9 and 12 months. Mixed regression analysis (quality of life) was used. Outcome assessment was conducted by researchers blinded to group assignment. RESULTS After 12 months, 22 (52%) patients had an all-cause re-admission or died. Only 3 patients were hospitalised with HF decompensation. No significant effect of the intervention was found on HF related to quality of life. CONCLUSIONS An inter-professional disease management programme is possible in the Swiss healthcare setting but effects on outcomes need to be confirmed in larger studies. https://sonar.ch/global/documents/12416 eng info:eu-repo/semantics/altIdentifier/doi/10.4414/smw.2011.13171 info:eu-repo/semantics/altIdentifier/pmid/21384285 Swiss medical weekly. - 2011 Aged Aged, 80 and over Ambulatory Care Disease Management Female Heart Failure Hospitalization Humans Length of Stay Male Patient Care Team Patient Education as Topic Patient Readmission Quality of Life Regression Analysis Switzerland Treatment Outcome Swiss Interdisciplinary Management Programme for Heart Failure (SWIM-HF): a randomised controlled trial study of an outpatient inter-professional management programme for heart failure patients in Switzerland. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12448 2025-10-24T20:47:33Z

Berner D Kaeuffer R Grandchamp AC Raeymaekers JA Räsänen K Hendry AP 2011 Ecological selection against hybrids between populations occupying different habitats might be an important component of reproductive isolation during the initial stages of speciation. The strength and directionality of this barrier to gene flow depends on the genetic architecture underlying divergence in ecologically relevant phenotypes. We here present line cross analyses of inheritance for two key foraging-related morphological traits involved in adaptive divergence between stickleback ecotypes residing parapatrically in lake and stream habitats within the Misty Lake watershed (Vancouver Island, Canada). One main finding is the striking genetic dominance of the lake phenotype for body depth. Selection associated with this phenotype against first- and later-generation hybrids should therefore be asymmetric, hindering introgression from the lake to the stream population but not vice versa. Another main finding is that divergence in gill raker number is inherited additively and should therefore contribute symmetrically to reproductive isolation. Our study suggests that traits involved in adaptation might contribute to reproductive isolation qualitatively differently, depending on their mode of inheritance. application/pdf https://sonar.ch/global/documents/12448 eng info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1420-9101.2011.02330.x info:eu-repo/semantics/altIdentifier/pmid/21649765 info:eu-repo/semantics/openAccess Journal of evolutionary biology. - 2011 Animals British Columbia Ecotype Female Gills Lakes Male Quantitative Trait, Heritable Reproductive Isolation Rivers Smegmamorpha Quantitative genetic inheritance of morphological divergence in a lake-stream stickleback ecotype pair: implications for reproductive isolation. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12449 2025-10-24T20:47:34Z

Rudin D Grize L Schindler C Marsch S Rüegg S Sutter R 2011 BACKGROUND Status epilepticus is one of the most important neurological emergencies and requires immediate therapy and admission to the intensive care unit. We hypothesized that nonconvulsive and subtle status epilepticus are more frequent than reported. METHODS This observational cohort study describes types, courses, duration, length of hospital stay, outcome and case fatality rate of status epilepticus in adults in relation to demographic and clinical variables. It was conducted in an intensive care unit of a tertiary care center over three years. RESULTS 111 status epilepticus episodes had a median duration of 48h. Hospitalization length was 18±15.3 days. 81% of the status epilepticus episodes were nonconvulsive and subtle. Case fatality rate was 17%. Age over 70 years had independent positive influence on status epilepticus course (OR: 5.135; p=0.0029). Hospital stay increased by 1.13h with each additional hour of status epilepticus (p=0.02). Subtle status epilepticus was a risk factor for refractoriness (p=0.0065). CONCLUSIONS Prevalence of nonconvulsive and subtle status epilepticus was higher than reported, emphasising the importance of clinical awareness. Older age was associated with more favorable course. This unexpected and contradictory result has to be taken into account during therapeutic interventions in the elderly and should warn from early resignation regarding treatment. https://sonar.ch/global/documents/12449 eng info:eu-repo/semantics/altIdentifier/doi/10.1016/j.eplepsyres.2011.05.018 info:eu-repo/semantics/altIdentifier/pmid/21676592 Epilepsy research. - 2011 Adult Aged Aged, 80 and over Algorithms Anticonvulsants Cohort Studies Electroencephalography Female Hospitalization Hospitals Humans Intensive Care Units Length of Stay Male Middle Aged Observation Outcome Assessment, Health Care Prevalence Retrospective Studies Statistics, Nonparametric Status Epilepticus Switzerland Young Adult High prevalence of nonconvulsive and subtle status epilepticus in an ICU of a tertiary care center: a three-year observational cohort study. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12383 2025-10-24T20:47:37Z

Borbély AA Tobler I 2011 https://sonar.ch/global/documents/12383 eng info:eu-repo/semantics/altIdentifier/doi/10.1016/B978-0-444-52006-7.00013-7 info:eu-repo/semantics/altIdentifier/pmid/21056188 Handbook of clinical neurology. - 2011 Brain Circadian Rhythm Electroencephalography Homeostasis Humans Models, Biological Sleep Manifestations and functional implications of sleep homeostasis. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12384 2025-10-24T20:47:37Z

Ohlerth S Becker-Birck M Augsburger H Jud R Makara M Braun U 2012 The present study was performed to provide computed tomographic (CT) reference values for structures in the thorax of 26 clinically normal Saanen goats. Animals were anesthetized, positioned in sternal recumbency and transverse images with a reconstructed 1.5mm slice thickness were obtained by use of a 40-slice CT scanner. Absolute and relative measurements of the trachea, heart, cranial vena cava, thoracic aorta, caudal vena cava, right and left principal bronchus, right and left caudal lobar bronchus and the concomitant branch of the right and left pulmonary artery and vein, large caudal mediastinal lymph node and lung density were performed with dedicated software. Minimal to moderate interstitial or bronchopneumonia was incidentally found on CT in 24 animals. In conclusion, CT images obtained in this study can be used as a reference for the evaluation of thoracic diseases in goats. application/pdf https://sonar.ch/global/documents/12384 eng info:eu-repo/semantics/altIdentifier/doi/10.1016/j.rvsc.2010.10.019 info:eu-repo/semantics/altIdentifier/pmid/21071049 info:eu-repo/semantics/openAccess Research in veterinary science. - 2012 Animals Female Goats Multidetector Computed Tomography Radiography, Thoracic Reference Values Thorax Computed tomography measurements of thoracic structures in 26 clinically normal goats. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12397 2025-10-24T20:47:39Z

von Teichman A Compérat E Behnke S Storz M Moch H Schraml P 2011 Multilocular cystic renal cell carcinoma is a rare renal cell carcinoma with an excellent prognosis. To clarify the relationship with typical clear cell renal cell carcinoma, we evaluated 15 cases of multilocular cystic renal cell carcinomas diagnosed according to the 2004 WHO classification. Von Hippel Lindau (VHL) gene mutations were determined by whole genome amplification and direct sequencing. Carbonic anhydrase 9 (CAIX), a hypoxia-inducible factor (HIF) target, paired box gene 2 (PAX2), cyclin-dependent kinase inhibitor p27 and glycogen synthase kinase 3-β (GSK3β) were immunohistochemically evaluated as members of the VHL protein (pVHL)- and phosphatase and tensin homolog (PTEN)-controlled pathways. VHL mutations were identified in 3 of 12 (25%) tumors. Inactivated GSK3β, decreased PTEN expression and PAX2 positivity were observed in the vast majority of the multilocular cystic renal cell carcinomas. Strong nuclear staining of p27 was seen in 14 of 15 cases. Compared with multilocular cystic renal cell carcinomas, expression frequencies of PAX2, p-GSK3β, PTEN and CAIX were similar in a set of low-grade, early-stage clear cell renal cell carcinomas, whereas only 30% had strong p27 positivity. These results are consistent with the hypothesis that multilocular cystic renal cell carcinomas are related at the molecular level with clear cell renal cell carcinomas. Maintenance of a strong subcellular p27 expression in all multilocular cystic renal cell carcinomas analyzed may in part explain the excellent prognosis of these tumor patients. application/pdf https://sonar.ch/global/documents/12397 eng info:eu-repo/semantics/altIdentifier/doi/10.1038/modpathol.2010.222 info:eu-repo/semantics/altIdentifier/pmid/21151099 info:eu-repo/semantics/openAccess Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc. - 2011 Antigens, Neoplasm Carbonic Anhydrase IX Carbonic Anhydrases Carcinoma, Renal Cell Chi-Square Distribution Cyclin-Dependent Kinase Inhibitor p27 DNA Mutational Analysis Glycogen Synthase Kinase 3 Glycogen Synthase Kinase 3 beta Humans Immunohistochemistry Kidney Neoplasms Mutation Neoplasm Staging Neoplasms, Cystic, Mucinous, and Serous PAX2 Transcription Factor PTEN Phosphohydrolase Phosphorylation Signal Transduction Von Hippel-Lindau Tumor Suppressor Protein VHL mutations and dysregulation of pVHL- and PTEN-controlled pathways in multilocular cystic renal cell carcinoma. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12458 2025-10-24T20:47:42Z

Betschart C Scheiner D Hess E Seifert B Fink D Perucchini D 2011 INTRODUCTION AND HYPOTHESIS The aim of the Incontinence Outcome Questionnaire (IOQ) is to assess quality of life and patient-reported outcome after midurethral slings. METHODS In this retrospective study, 626 patients with a minimum follow-up period of 1 year were sent the IOQ. Four hundred twenty-two of 626 (67.4%) patient responses were evaluated. RESULTS The mean IOQ QoL extended score was 33.7 ± 17.5 and comparable for tension-free vaginal tape (TVT), outside-in transobturator tape (TOT), and inside-out transobturator vaginal tape (TVT-O). Evaluation of IOQ question about readmission revealed a total of 32 patients (18 TVT, 12 TOT, and 2 TVT-O) who underwent a subsequent operation due to sling-related complications. Freedom from reoperation for recurrent SUI at 1, 2, and 5 years was 100%, 99.7 ± 0.3%, and 99.7 ± 0.3% for TVT, 100% for TVT-O, and 94.2 ± 2.5%, 91.9 ± 2.9%, and 89.9 ± 3.5% for TOT, respectively (p < 0.001). CONCLUSIONS Patient satisfaction, assessed using the IOQ, is high after retropubic and transobturator slings. In our collective, relapse incontinence is higher after TOT. application/pdf https://sonar.ch/global/documents/12458 eng info:eu-repo/semantics/altIdentifier/doi/10.1007/s00192-011-1366-7 info:eu-repo/semantics/altIdentifier/pmid/21318441 info:eu-repo/semantics/openAccess International urogynecology journal. - 2011 Aged Female Humans Middle Aged Patient Readmission Patient Satisfaction Quality of Life Reoperation Retrospective Studies Suburethral Slings Surveys and Questionnaires Urinary Incontinence Urologic Surgical Procedures Patient satisfaction after retropubic and transobturator slings: first assessment using the Incontinence Outcome Questionnaire (IOQ). http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12479 2025-10-24T20:47:44Z

Bosshart H Heinzelmann M 2011 In the continuous presence of bacterial lipopolysaccharide (LPS), the membrane-bound LPS receptor, CD14, exhibits a biphasic pattern of surface expression in monocytes ex vivo; an initial increase is followed by a later decrease. In the presence of LPS, ex vivo changes in monocytic CD14 cell surface expression have been consistently interpreted as the direct result of LPS exposure. There has been little consideration for the possibility of additional cell culture effects. Here, an experiment is presented to dissect the differences between LPS effects and cell culture effects on monocytic CD14 cell surface expression ex vivo. The results show that in monocytes from diluted whole blood cultures, CD14 surface expression is induced in LPS-treated samples but decreased in untreated samples. These observations suggest that the previously observed biphasic surface expression pattern of CD14 in long-term LPS-treated monocytes may be the result of a superposition of LPS-induced expression and spontaneous disappearance of CD14 from the plasma membrane. Further, these results illustrate the importance of taking cell culture conditions into account when analyzing monocyte expression of CD14. https://sonar.ch/global/documents/12479 eng info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jim.2011.03.006 info:eu-repo/semantics/altIdentifier/pmid/21453706 Journal of immunological methods. - 2011 Cell Culture Techniques Female Flow Cytometry Humans Immunophenotyping Leukocytes, Mononuclear Lipopolysaccharide Receptors Lipopolysaccharides Male Spontaneous decrease of CD14 cell surface expression in human peripheral blood monocytes ex vivo. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12499 2025-10-24T20:47:45Z

Wenger M Sattler U Goldschmidt-Clermont E Segner H 2011 Research on the endocrine role of estrogens has focused on the reproductive system, while other potential target systems have been less studied. Here, we investigated the possible immunomodulating role of 17β-estradiol (E2) using rainbow trout (Oncorhynchus mykiss) as a model. The aims of the study were to examine a) whether estrogens can modulate immune gene transcription levels, and b) whether this has functional implications for the resistance of trout towards pathogens. Trout were reared from fertilization until 6 months of age under (1) control conditions, (2) short-term E2-treatment (6-month-old juveniles were fed a diet containing 20 mg E2/kg for 2 weeks), or c) long-term E2-treatment (twice a 2-h-bath-exposure of trout embryos to 400 μg 17β-estradiol (E2)/L, followed by rearing on the E2-spiked diet from start-feeding until 6 months of age). Analysis of plasma estrogen levels indicated that the internal estrogen concentrations of E2-exposed fish were within the physiological range and analysis of hepatic vitellogenin mRNA levels indicated that the E2 administration was effective in activating the endogenous estrogen receptor pathway. However, expression levels of the hepatic complement components C3-1, C3-3, and Factor H were not affected by E2-treatment. In a next step, 6-month-old juveniles were challenged with pathogenic bacteria (Yersinia ruckeri). In control fish, this bacterial infection resulted in significant up-regulation of the mRNA levels of hepatic complement genes (C3-1, C3-3, Factor B, Factor H), while E2-treated fish showed no or significantly lower up-regulation of the complement gene transcription levels. Apparently, the E2-treated trout had a lower capacity to activate their immune system to defend against the bacterial infection. This interpretation is corroborated by the finding that survival of E2-treated fish under bacterial challenge was significantly lower than in the control group. In conclusion, the results from this study suggest that estrogens are able to modulate immune parameters of trout with functional consequences on their ability to cope with pathogens. https://sonar.ch/global/documents/12499 eng info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fsi.2011.04.007 info:eu-repo/semantics/altIdentifier/pmid/21549195 Fish & shellfish immunology. - 2011 Animals Complement System Proteins Estradiol Female Fish Diseases Fish Proteins Gene Expression Profiling Immunity, Innate Liver Oncorhynchus mykiss RNA, Messenger Vitellogenins Yersinia Infections Yersinia ruckeri 17Beta-estradiol affects the response of complement components and survival of rainbow trout (Oncorhynchus mykiss) challenged by bacterial infection. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12528 2025-10-24T20:47:47Z

Ster J Mateos JM Grewe BF Coiret G Corti C Corsi M Helmchen F Gerber U 2011 Impaired function or expression of group II metabotropic glutamate receptors (mGluRIIs) is observed in brain disorders such as schizophrenia. This class of receptor is thought to modulate activity of neuronal circuits primarily by inhibiting neurotransmitter release. Here, we characterize a postsynaptic excitatory response mediated by somato-dendritic mGluRIIs in hippocampal CA3 pyramidal cells and in stratum oriens interneurons. The specific mGluRII agonists DCG-IV or LCCG-1 induced an inward current blocked by the mGluRII antagonist LY341495. Experiments with transgenic mice revealed a significant reduction of the inward current in mGluR3(-/-) but not in mGluR2(-/-) mice. The excitatory response was associated with periods of synchronized activity at theta frequency. Furthermore, cholinergically induced network oscillations exhibited decreased frequency when mGluRIIs were blocked. Thus, our data indicate that hippocampal responses are modulated not only by presynaptic mGluRIIs that reduce glutamate release but also by postsynaptic mGluRIIs that depolarize neurons and enhance CA3 network activity. application/pdf https://sonar.ch/global/documents/12528 eng info:eu-repo/semantics/altIdentifier/doi/10.1073/pnas.1100548108 info:eu-repo/semantics/altIdentifier/pmid/21628565 info:eu-repo/semantics/openAccess Proceedings of the National Academy of Sciences of the United States of America. - 2011 Action Potentials Amino Acids Animals CA3 Region, Hippocampal Cyclopropanes Excitatory Amino Acid Agonists Excitatory Amino Acid Antagonists Excitatory Postsynaptic Potentials Glycine Interneurons Mice Mice, Knockout Microscopy, Electron Nerve Net Patch-Clamp Techniques Pyramidal Cells Rats Rats, Wistar Receptors, Metabotropic Glutamate Theta Rhythm Xanthenes Enhancement of CA3 hippocampal network activity by activation of group II metabotropic glutamate receptors. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12403 2025-10-24T20:47:49Z

Tzankov A Sotlar K Muhlematter D Theocharides A Went P Jotterand M Horny HP Dirnhofer S 2008 Systemic mastocytoses represent neoplastic proliferations of mast cells. In about 20% of cases systemic mastocytoses are accompanied by clonal haematopoietic non-mast cell-lineage disorders, most commonly myeloid neoplasms. A case of systemic mastocytosis carrying the characteristic mutation at codon 816 (D816V) in the KIT gene of mast cells, with two concurrent accompanying clonal haematopoietic non-mast cell-lineage disorders, chronic myeloproliferative disease, unclassifiable and precursor B lymphoblastic leukaemia is documented. Both accompanying clonal haematopoietic non-mast cell-lineage disorders carried the wild-type KIT gene, but had a novel t(13;13)(q12;q22) involving the FLT3 locus at 13q12. The chronic myeloproliferative disease, unclassifiable and the precursor B lymphoblastic leukaemia were cured by syngenous stem cell transplantation, but the systemic mastocytosis persisted for more than 10 years. The additional impact of molecular techniques on the correct diagnosis in haematological malignancies is highlighted, and evidence is provided that, apart from internal tandem duplications and mutations, FLT3 can be activated by translocations. application/pdf https://sonar.ch/global/documents/12403 eng info:eu-repo/semantics/altIdentifier/doi/10.1136/jcp.2008.058073 info:eu-repo/semantics/altIdentifier/pmid/18663058 info:eu-repo/semantics/openAccess Journal of clinical pathology. - 2008 Chromosomes, Human, Pair 13 Humans In Situ Hybridization, Fluorescence Karyotyping Male Mastocytosis, Systemic Middle Aged Myeloproliferative Disorders Peripheral Blood Stem Cell Transplantation Precursor B-Cell Lymphoblastic Leukemia-Lymphoma Translocation, Genetic fms-Like Tyrosine Kinase 3 Systemic mastocytosis with associated myeloproliferative disease and precursor B lymphoblastic leukaemia with t(13;13)(q12;q22) involving FLT3. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12425 2025-10-24T20:47:51Z

Blum JA Zellweger MJ Burri C Hatz C 2008 American trypanosomiasis (Chagas disease) and human African trypanosomiasis (HAT; sleeping sickness) are both caused by single-celled flagellates that are transmitted by arthropods. Cardiac problems are the main cause of morbidity in chronic Chagas disease, but neurological problems dominate in HAT. Physicians need to be aware of Chagas disease and HAT in patients living in or returning from endemic regions, even if they left those regions long ago. Chagas heart disease has to be taken into account in the differential diagnosis of cardiomyopathy, primarily in patients with pathological electrocardiographic (ECG) findings, such as right bundle branch block or left anterior hemiblock, with segmental wall motion abnormalities or aneurysms on echocardiography, and in young patients with stroke in the absence of arterial hypertension. In HAT patients, cardiac involvement as seen by ECG alterations, such as repolarisation changes and low voltage, is frequent. HAT cardiopathy in general is benign and does not cause relevant congestive heart failure and subsides with treatment. We review the differences between the American and African trypanosomiasis with the main focus on the heart. https://sonar.ch/global/documents/12425 eng info:eu-repo/semantics/altIdentifier/doi/10.1016/S1473-3099(08)70230-5 info:eu-repo/semantics/altIdentifier/pmid/18922485 The Lancet. Infectious diseases. - 2008 Chagas Disease Heart Diseases Humans Trypanosomiasis, African Cardiac involvement in African and American trypanosomiasis. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12460 2025-10-24T20:47:55Z

Hunziker S Tschan F Semmer NK Zobrist R Spychiger M Breuer M Hunziker PR Marsch SC 2009 BACKGROUND Cardiac arrests are handled by teams rather than by individual health-care workers. Recent investigations demonstrate that adherence to CPR guidelines can be less than optimal, that deviations from treatment algorithms are associated with lower survival rates, and that deficits in performance are associated with shortcomings in the process of team-building. The aim of this study was to explore and quantify the effects of ad-hoc team-building on the adherence to the algorithms of CPR among two types of physicians that play an important role as first responders during CPR: general practitioners and hospital physicians. METHODS To unmask team-building this prospective randomised study compared the performance of preformed teams, i.e. teams that had undergone their process of team-building prior to the onset of a cardiac arrest, with that of teams that had to form ad-hoc during the cardiac arrest. 50 teams consisting of three general practitioners each and 50 teams consisting of three hospital physicians each, were randomised to two different versions of a simulated witnessed cardiac arrest: the arrest occurred either in the presence of only one physician while the remaining two physicians were summoned to help ("ad-hoc"), or it occurred in the presence of all three physicians ("preformed"). All scenarios were videotaped and performance was analysed post-hoc by two independent observers. RESULTS Compared to preformed teams, ad-hoc forming teams had less hands-on time during the first 180 seconds of the arrest (93 +/- 37 vs. 124 +/- 33 sec, P < 0.0001), delayed their first defibrillation (67 +/- 42 vs. 107 +/- 46 sec, P < 0.0001), and made less leadership statements (15 +/- 5 vs. 21 +/- 6, P < 0.0001). CONCLUSION Hands-on time and time to defibrillation, two performance markers of CPR with a proven relevance for medical outcome, are negatively affected by shortcomings in the process of ad-hoc team-building and particularly deficits in leadership. Team-building has thus to be regarded as an additional task imposed on teams forming ad-hoc during CPR. All physicians should be aware that early structuring of the own team is a prerequisite for timely and effective execution of CPR. https://sonar.ch/global/documents/12460 eng info:eu-repo/semantics/altIdentifier/doi/10.1186/1471-227X-9-3 info:eu-repo/semantics/altIdentifier/pmid/19216796 BMC emergency medicine. - 2009 Adult Algorithms Cardiopulmonary Resuscitation Communication Female Guideline Adherence Humans Male Middle Aged Patient Care Team Patient Simulation Prospective Studies Surveys and Questionnaires Time Factors Videotape Recording Hands-on time during cardiopulmonary resuscitation is affected by the process of teambuilding: a prospective randomised simulator-based trial. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12476 2025-10-24T20:47:57Z

Rickenbacher I Gugleta K Zawinka C Schötzau A Katamay R Flammer J Orgül S 2009 BACKGROUND Vascular dysregulation is considered to be a risk factor in several ophthalmic diseases. The purpose of this study was to evaluate the reaction of retinal vessels to flicker light in otherwise healthy subjects with a vasospastic propensity. PATIENTS AND METHODS Thirty healthy Caucasians, aged between 18-35 years were recruited for this study and grouped into vasospastics, based on a history of frequent cold hands, even in summer, with concordant findings in nailfold capillary microscopy, or as controls, if such a history was absent. The reaction of the retinal vascular diameter to flicker light was observed in a distance of two to three discs diameters away from the optic nerve head with the retinal vessel analyser. Three phases of flicker light of twenty seconds followed by baseline light phases of eighty seconds were recorded. The maximal vasodilatory amplitude of each flicker phase was determined and the results averaged. RESULTS The maximal average dilatory amplitude at the arterial side reached (mean +/- SD) 2.9 +/- 1.7 % and 4.8 +/- 2.6 % of the baseline amplitude respectively in vasospastic subjects and in healthy controls (t = 2.34; p = 0.025). The reaction at the venous side was statistically comparable in both groups. CONCLUSIONS Otherwise healthy, vasospastic subject disclosed an altered reaction of the retinal vasculature to flicker light in this study. https://sonar.ch/global/documents/12476 eng info:eu-repo/semantics/altIdentifier/doi/10.1055/s-0028-1109271 info:eu-repo/semantics/altIdentifier/pmid/19384788 Klinische Monatsblatter fur Augenheilkunde. - 2009 Adolescent Adult Female Humans Male Photic Stimulation Retinal Vessels Vasospasm, Intracranial Young Adult [Response of retinal vessel diameter to flicker-light in vasospastics compared to healthy controls]. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12484 2025-10-24T20:47:58Z

Dongus S Nyika D Kannady K Mtasiwa D Mshinda H Gosoniu L Drescher AW Fillinger U Tanner M Killeen GF Castro MC 2009 A cross-sectional survey of agricultural areas, combined with routinely monitored mosquito larval information, was conducted in urban Dar es Salaam, Tanzania, to investigate how agricultural and geographical features may influence the presence of Anopheles larvae. Data were integrated into a geographical information systems framework, and predictors of the presence of Anopheles larvae in farming areas were assessed using multivariate logistic regression with independent random effects. It was found that more than 5% of the study area (total size 16.8 km2) was used for farming in backyard gardens and larger open spaces. The proportion of habitats containing Anopheles larvae was 1.7 times higher in agricultural areas compared to other areas (95% confidence interval = 1.56-1.92). Significant geographic predictors of the presence of Anopheles larvae in gardens included location in lowland areas, proximity to river, and relatively impermeable soils. Agriculture-related predictors comprised specific seedbed types, mid-sized gardens, irrigation by wells, as well as cultivation of sugar cane or leafy vegetables. Negative predictors included small garden size, irrigation by tap water, rainfed production and cultivation of leguminous crops or fruit trees. Although there was an increased chance of finding Anopheles larvae in agricultural sites, it was found that breeding sites originated by urban agriculture account for less than a fifth of all breeding sites of malaria vectors in Dar es Salaam. It is suggested that strategies comprising an integrated malaria control effort in malaria-endemic African cities include participatory involvement of farmers by planting shade trees near larval habitats. application/pdf https://sonar.ch/global/documents/12484 eng info:eu-repo/semantics/altIdentifier/doi/10.4081/gh.2009.220 info:eu-repo/semantics/altIdentifier/pmid/19440962 info:eu-repo/semantics/openAccess Geospatial health. - 2009 Animals Anopheles Cross-Sectional Studies Ecosystem Gardening Larva Logistic Models Parasite Egg Count Tanzania Urban Population Urban agriculture and Anopheles habitats in Dar es Salaam, Tanzania. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12500 2025-10-24T20:47:59Z

Bozic-Weber B Constable EC Housecroft CE Kopecky P Neuburger M Zampese JA 2011 6-(1-Methylpyrrol-2-yl)-2,2'-bipyridine, 3, and 6-(selenophene-2-yl)-2,2'-bipyridine, 4, have been prepared and characterized in solution and by structural determinations. Copper(I) complexes [CuL(2)][PF(6)] in which L is 2,2'-bipyridine substituted in the 6-position by furyl, thienyl, N-methylpyrrolyl, selenopheneyl, methyl or phenyl, (L = 1-6) have been synthesized. The complexes have been characterized by electrospray mass spectrometry, and solution NMR and UV-VIS spectroscopies. The single crystal structures of [Cu(1)(2)][PF(6)], [Cu(2)(2)][PF(6)], [Cu(3)(2)][PF(6)], [Cu(5)(2)][PF(6)] and [Cu(6)(2)][PF(6)] have been determined. In those compounds containing an aromatic substituent attached to the bpy unit, the substituent is twisted with respect to the latter. In [Cu(3)(2)][PF(6)] and [Cu(5)(2)][PF(6)], this results in intra-cation π-stacking between ligands which is very efficient in [Cu(3)(2)](+) despite the steric requirements of the N-methyl substituents. Face-to-face stacking between the ligands in the [Cu(2)(2)](+) ion is achieved by complementary substituent twisting and elongation of one Cu-N bond, but there is no analogous intra-cation π-stacking in [Cu(1)(2)](+). Ligand exchange reactions between [CuL(2)][PF(6)] (L = 1-6) and TiO(2)-anchored ligands 7-10 (L' = 2,2'-bipyridine-based ligands with CO(2)H or PO(OH)(2) anchoring groups) have been applied to produce 24 surface-anchored heteroleptic copper(i) complexes, the formation of which has been evidenced by using MALDI-TOF mass spectrometry and thin layer solid state diffuse reflectance electronic absorption spectroscopy. The efficiencies of the complexes as dyes in DSCs have been measured, and the best efficiencies are observed for [CuLL'] with L' = 10 which contains phosphonate anchoring groups. https://sonar.ch/global/documents/12500 eng info:eu-repo/semantics/altIdentifier/doi/10.1039/c1dt11052g info:eu-repo/semantics/altIdentifier/pmid/21993898 Dalton transactions (Cambridge, England : 2003). - 2011 The intramolecular aryl embrace: from light emission to light absorption. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12509 2025-10-24T20:48:01Z

Rovó A Tichelli A 2012 Cardiovascular diseases (CVD) are emerging late effects after allogeneic hematopoietic stem cell transplantation (allo-HSCT), leading to considerable morbidity and mortality. These late CVD are in most cases related to enhanced atherosclerosis, promoted by the early appearance after transplantation of cardiovascular risk factors. According to the data obtained from the general population it is very likely that early intervention on these cardiovascular risk factors might defer the appearance of late CVD. This review focuses on the published data of cardiovascular diseases after transplantation, the potential associated risk factors, and the postulated pathophysiological mechanisms. A suggested approach for early identification of patients at risk, optimal surveillance, and screening of the modifiable cardiovascular risk factors and the possible early interventions are also discussed here. Long-term survivors should be assessed lifelong after HSCT; all healthcare providers involved in the follow-up of these patients should be aware of premature health threatening of cardiovascular diseases after transplantation. https://sonar.ch/global/documents/12509 eng info:eu-repo/semantics/altIdentifier/doi/10.1053/j.seminhematol.2011.10.001 info:eu-repo/semantics/altIdentifier/pmid/22221782 Seminars in hematology. - 2012 Cardiovascular Diseases Follow-Up Studies Hematopoietic Stem Cell Transplantation Humans Risk Factors Survivors Time Factors Transplantation, Homologous Cardiovascular complications in long-term survivors after allogeneic hematopoietic stem cell transplantation. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12517 2025-10-24T20:48:03Z

Güven S Karagianni M Schwalbe M Schreiner S Farhadi J Bula S Bieback K Martin I Scherberich A 2012 The stromal vascular fraction of adipose tissue has gained popularity as a source of autologous progenitor cells for tissue engineering and regenerative medicine applications. The aim of this study was to validate a newly developed, automated procedure to isolate adipose-derived mesenchymal stem/stromal cells (ASCs) from adult human lipoaspirates in a closed and clinical-grade device, based on the Sepax(®) technology. Using a total of 11 donors, this procedure was compared with the standard operator-based manual separation in terms of isolation yield, clonogenic fraction, phenotype, and differentiation potential of ASCs. As compared with the manual process, automation resulted in a 62% higher isolation yield, with 2.6±1.2×10(5) nucleated cells per mL of liposuction, and a 24% higher frequency of clonogenic progenitors. The variability in the isolation yield and clonogenicity across different preparations was reduced by 18% and 50%, respectively. The cytofluorimetric profile and in vitro differentiation capacity into mesenchymal lineages were comparable in the cells isolated using the two procedures. The new Sepax-based process thus allows an efficient isolation of ASCs with higher and more reproducible yields than the standard manual procedure, along with minimal operator intervention. These results are expected to facilitate the use of ASCs for clinical purposes, either within an intraoperative setting or in combination with further in vitro cell expansion/cultivation. application/pdf https://sonar.ch/global/documents/12517 eng info:eu-repo/semantics/altIdentifier/doi/10.1089/ten.TEC.2011.0617 info:eu-repo/semantics/altIdentifier/pmid/22372873 info:eu-repo/semantics/openAccess Tissue engineering. Part C, Methods. - 2012 Adipose Tissue Adult Aged Automation Cell Culture Techniques Cell Differentiation Cells, Cultured Chondrocytes Female Flow Cytometry Humans Lipectomy Mesoderm Middle Aged Osteoblasts Phenotype Stem Cells Tissue Culture Techniques Validation of an automated procedure to isolate human adipose tissue-derived cells by using the Sepax® technology. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12550 2025-10-24T20:48:06Z

Ochoa-Espinosa A Baer MM Affolter M 2012 New work shows the instructive role of Src42A kinase in tube size regulation. By inducing polarized cell-shape changes, Src42A promotes tube elongation in the Drosophila tracheal system. application/pdf https://sonar.ch/global/documents/12550 eng info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cub.2012.04.033 info:eu-repo/semantics/altIdentifier/pmid/22677286 info:eu-repo/semantics/openAccess Current biology : CB. - 2012 Animals Drosophila Drosophila Proteins Malpighian Tubules Proto-Oncogene Proteins pp60(c-src) Tubulogenesis: Src42A goes to great lengths in tube elongation. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12553 2025-10-24T20:48:13Z

Duong FH Christen V Lin S Heim MH 2010 UNLABELLED The molecular mechanisms underlying hepatocarcinogenesis in chronic viral hepatitis are poorly understood. A potential tumorigenic pathway could involve protein phosphatase 2A (PP2A) and protein arginine methyltransferase 1 (PRMT1), because both enzymes are dysregulated in chronic hepatitis C, and both enzymes have been involved in chromatin remodeling and DNA damage repair. We used cell lines that allow the inducible expression of hepatitis C virus proteins (UHCV57.3) and of the catalytic subunit of PP2A (UPP2A-C8) as well as Huh7.5 cells infected with recombinant cell culture-derived hepatitis C virus (HCVcc) to study epigenetic histone modifications and DNA damage repair. The induction of viral proteins, the overexpression of PP2Ac, or the infection of Huh7.5 cells with HCVcc resulted in an inhibition of histone H4 methylation/acetylation and histone H2AX phosphorylation, in a significantly changed expression of genes important for hepatocarcinogenesis, and inhibited DNA damage repair. Overexpression of PP2Ac in NIH-3T3 cells increased anchorage-independent growth. These changes were partially reversed by the treatment of cells with the methyl-group donor S-adenosyl-L-methionine (SAMe). CONCLUSION Hepatitis C virus-induced overexpression of PP2Ac contributes to hepatocarcinogenesis through dysregulation of epigenetic histone modifications. The correction of defective histone modifications by S-adenosyl-L-methionine makes this drug a candidate for chemopreventive therapies in patients with chronic hepatitis C who are at risk for developing hepatocellular carcinoma. https://sonar.ch/global/documents/12553 eng info:eu-repo/semantics/altIdentifier/doi/10.1002/hep.23388 info:eu-repo/semantics/altIdentifier/pmid/20043320 Hepatology (Baltimore, Md.). - 2010 Cells, Cultured DNA Repair Hepacivirus Histones Humans Liver Neoplasms Protein Phosphatase 2 Up-Regulation Hepatitis C virus-induced up-regulation of protein phosphatase 2A inhibits histone modification and DNA damage repair. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12570 2025-10-24T20:48:15Z

Steffel J Rempel H Breitenstein A Schmidt S Namdar M Krasniqi N Holzmeister J Lüscher TF Ruschitzka F Hürlimann D 2014 BACKGROUND Cardiac resynchronization therapy (CRT) reduces morbidity and mortality in patients suffering from chronic heart failure (CHF). Optimal device programming is crucial for maximum patient benefit. The goal of the present study was to assess device settings from CHF patients undergoing CRT optimization in a "real world" setting, and to delineate parameters most frequently requiring adjustment. METHODS All patients who underwent CRT device implantation in the Cardiology Clinicat the University Hospital Zurich between January 2011 and September 2012 and in whom follow-up was available were included in this analysis. RESULTS A total of 170 CHF patients were included in this analysis. True biventricular pacing was present in 44% of all patients, while QRS fusion was detected in 49.9%. The majority of the patients presented with suboptimal atrioventricular (AV) delays requiring adjustment. AV delays were therefore shortened due to the presence of QRS fusion in 53.3% and 38.1% of patients (sAV and pAV, respectively) or prolonged because of truncation of the A wave in the left ventricular inflow pulse wave Doppler measurement (17.5% and 28.4% for sAV and pAV, respectively). In contrast, interventricular delay (VV delay) was rarely changed (11.9%). CONCLUSIONS In our "real world" cohort, a substantial proportion of patients presented to their first post-operative consultation with suboptimal device settings. Our data indicate that the opportunity to optimize device settings is frequently wasted in the "real world", underlining the necessity for expert device follow-up to deliver optimal care to this challenging group of heart failure patients. application/pdf https://sonar.ch/global/documents/12570 eng info:eu-repo/semantics/altIdentifier/doi/10.5603/CJ.a2013.0123 info:eu-repo/semantics/altIdentifier/pmid/23990194 info:eu-repo/semantics/openAccess Cardiology journal. - 2014 Cardiac Resynchronization Therapy Echocardiography, Doppler Female Follow-Up Studies Heart Failure Heart Rate Heart Ventricles Humans Male Middle Aged Prospective Studies Treatment Outcome Comprehensive cardiac resynchronization therapy optimization in the real world. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12577 2025-10-24T20:48:17Z

Auer JA 2011 The first meeting leading to the formation of the European College of Veterinary Surgeons (ECVS) was held August 30/31, 1990 in Lenzburg, Switzerland. Specialists in small animal and large animal surgery from practice and academicians from many European countries were invited. The constitution was developed based on that of the American College of Veterinary Surgeons (ACVS). ECVS was founded during the ACVS European Surgical Forum in Nice in 1991. The provisional Board elected at this meeting stayed active during the initial 4 years to provide stability. For the 146 Charter Members to become a Diplomate, they had to pass an examination. Therefore, the ECVS is the only Specialty College that does not have a "Grand Father Clause." In 2000 ECVS, was the first College to acquire full recognition-status by the European Board of Specialization (EBVS), which underlines the leading role the surgeons play in the development of the European veterinary specialty colleges. Like its American Sister College, ECVS maintains high standards for surgical training programs for Residents and administers rigorous certifying examinations. It is appropriate to recall the facts that lead to foundation of ECVS on its 20-year anniversary. https://sonar.ch/global/documents/12577 eng info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1532-950X.2011.00865.x info:eu-repo/semantics/altIdentifier/pmid/21815897 Veterinary surgery : VS. - 2011 Certification Education, Graduate Education, Veterinary Europe History, 20th Century History, 21st Century Societies, Scientific Surgery, Veterinary The European College of Veterinary Surgeons 1991-2011: a 20-year success story. http://purl.org/coar/resource_type/c_6501

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Khaiseb S Sydler T Zimmermann D Pospischil A Sidler X Brugnera E 2011 A member of the family Circoviridae, porcine circovirus type 2 (PCV2), is associated with postweaning multisystemic wasting syndrome (PMWS), a recent emerging disease worldwide. PCV2 is also found in clinically asymptomatic animals. This paradoxical finding makes the syndrome etiology challenging. We developed new assays to study PCV2 with links to syndrome etiology. For analysis, we used PCV2-infected tissues from subclinically infected and diseased piglets. We compared antigen- and PCV2 DNA-derived signals for tissue localization and intensity. Oligonucleotides were designed to the signature motif of the PCV2 capsid open reading frame to discriminate experimentally between PCV2 genotype groups by PCR, in situ hybridization (ISH), and fluorescence in situ hybridization (FISH). Unexpectedly, all PCV2-infected animals carried both PCV2a and PCV2b genotype group members. Using confocal microscopy, genotype single-cell infections and cell superinfections were visible. Additionally, we discriminated replicative DNA from total PCV2 DNA isoforms with FISH. This aided in our inquiry into cellular genotype-specific replication. Importantly, single-genotype-group replication was not observed. In infected cells with replicating virus, both genotype groups were equally present. These findings suggest PCV2 genotype group members relaxed replication regulation requirements and may even point to PCV2 replication cooperativity in vivo. These observations explain the readily seen PCV2 DNA recombinations and the high overall PCV2 genome plasticity. Hence, we suggest a novel mechanism of syndrome etiology that consists of a continuously changing PCV2 genome pool in hosts and pig herds, posing a constant challenge to the individual maturing immune system. application/pdf https://sonar.ch/global/documents/12585 eng info:eu-repo/semantics/altIdentifier/doi/10.1128/JVI.05156-11 info:eu-repo/semantics/altIdentifier/pmid/21865380 info:eu-repo/semantics/openAccess Journal of virology. - 2011 Animals Capsid Proteins Circovirus DNA, Viral Evolution, Molecular Genotype In Situ Hybridization Microscopy, Confocal Polymerase Chain Reaction Porcine Postweaning Multisystemic Wasting Syndrome Swine Virus Replication Coreplication of the major genotype group members of porcine circovirus type 2 as a prerequisite to coevolution may explain the variable disease manifestations. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12598 2025-10-24T20:48:23Z

Bielicki JA Achermann R Berger C 2013 OBJECTIVE Incomplete and delayed vaccination is a barrier to individual and population protection from vaccine-preventable diseases. We aimed to assess visit frequency and pattern in relation to vaccination status in a Swiss cohort of 2-year-old children in order to review opportunities for completion of scheduled immunizations. METHOD A retrospective dynamic cohort study design involving children insured with a single health insurer in Switzerland was chosen. Time-to-event analysis was used to evaluate timing of defined immunizations of interest from submitted invoices. Diphtheria, tetanus, acellular pertussis (DTaP) and measles, mumps and rubella (MMR) immunizations administered to children registered with this health insurer were assessed. The specified vaccines are recommended at 2, 4, 6 and 15-24 months, and 12 and 15-24 months of age, respectively. RESULTS 21,588 children born between January 1st, 2006 and June 30th, 2008 and registered with the health insurer from no later than 4 weeks of age were included. Only 40.9% of the cohort was up-to-date for both vaccines (DTaP and MMR) at 2 years of age. The average number of visits made during up to 2 years of age was 14.7 (95% CI: 15.9-16.3). Less than 5% of children made fewer than 5 visits, the minimum number required to complete all recommended immunizations by 2 years of age. Although number of visits varied by final vaccination status, more than 90% of the cohort made sufficient visits to complete the specified courses, even when contraindications were assumed to be present at up to half the visits. CONCLUSION Swiss children who are not fully immunized at 2 years of age make fewer visits to ambulatory health care up to that age, but they have more than sufficient opportunities to complete immunizations as scheduled. Ambulatory healthcare providers in Switzerland have ample opportunity to promote and administer vaccinations in a timely manner. https://sonar.ch/global/documents/12598 eng info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vaccine.2013.09.025 info:eu-repo/semantics/altIdentifier/pmid/24075922 Vaccine. - 2013 Immunization programme Organizational Case Study Physician's Practice Patterns Preventive Health Services Ambulatory Care Child, Preschool Cohort Studies Female Humans Immunization Schedule Infant Male Medication Adherence Office Visits Retrospective Studies Switzerland Vaccination In touch but not up-to-date: ambulatory visits and vaccination status in a cohort of young Swiss children. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12599 2025-10-24T20:48:23Z

Lachat M Veith FJ Pfammatter T Glenck M Bettex D Mayer D Rancic Z Gloekler S Pecoraro F 2013 PURPOSE To evaluate the performance of periscope and/or chimney grafts (CPGs) in the endovascular treatment of pararenal or thoracoabdominal aneurysms using off-the-shelf devices. METHODS Between February 2002 and August 2012, 77 consecutive patients (62 men; mean age 73±9 years) suffering from pararenal aortic (n=55), thoracoabdominal (n=16), or arch to visceral artery aneurysms (n=6) were treated with aortic stent-graft implantation requiring chimney and/or periscope grafts to maintain side branch perfusion. CPGs were planned in advance and were not used as bailout. A standardized follow-up protocol including computed tomographic angiography, laboratory testing, and clinical examination was performed at 6 weeks; 3, 6, and 12 months; and annually thereafter. RESULTS Technical success was achieved in 76 (99%) patients; 1 branch stent-graft became dislocated from a renal artery, which could not be re-accessed. Overall, 169 target vessels (121 renal arteries, 30 superior mesenteric arteries, 17 celiac trunks, and 1 inferior mesenteric artery) were addressed with the chimney graft configuration in 111 and the periscope graft configuration in 58. In total, 228 devices were used for the CPGs: 213 Viabahn stent-grafts and 15 bare metal stents. Over a mean 25±16 months (range 1-121), 9 patients died of unrelated causes. Nearly all (95%) of the patients demonstrated a decreased or stable aneurysm size on imaging; there was a mean 13% shrinkage in aneurysm diameter. Twenty patients had primary type I/III endoleaks at discharge; in follow-up, only 3 of these were still present (no secondary or recurrent endoleaks were noted). Additional endovascular maneuvers were required for CPG-related complications in 13 patients from intervention throughout follow-up. Overall, 4 CPGs occluded (98% target vessel patency); no stent-graft migration was observed. Renal function remained stable in all patients. CONCLUSION In this series, the use of CPGs has proven to be a feasible, safe, and effective way to treat thoracoabdominal and pararenal aneurysms with maintenance of blood flow to the renovisceral arteries. Nearly all of the aneurysms showed no increase in diameter over a >2-year mean follow-up, which supports the midterm adequacy of the CPG technique as a method to effectively revascularize branch vessels with few endoleaks or branch occlusions. application/pdf https://sonar.ch/global/documents/12599 eng info:eu-repo/semantics/altIdentifier/doi/10.1583/13-4372.1 info:eu-repo/semantics/altIdentifier/pmid/24093310 info:eu-repo/semantics/openAccess Journal of endovascular therapy : an official journal of the International Society of Endovascular Specialists. - 2013 Aged Aged, 80 and over Aortic Aneurysm, Abdominal Aortic Aneurysm, Thoracic Aortography Blood Vessel Prosthesis Blood Vessel Prosthesis Implantation Endoleak Endovascular Procedures Female Humans Kaplan-Meier Estimate Male Middle Aged Prosthesis Design Retrospective Studies Stents Time Factors Tomography, X-Ray Computed Treatment Outcome Vascular Patency Chimney and periscope grafts observed over 2 years after their use to revascularize 169 renovisceral branches in 77 patients with complex aortic aneurysms. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12603 2025-10-24T20:48:24Z

Leeners B Hengartner MP Rössler W Ajdacic-Gross V Angst J 2014 INTRODUCTION There are little published data on the prevalence of orgasmic difficulties in representative samples and no longitudinal data on the subject. In addition, our knowledge of the factors, which increase or protect against the risk of orgasmic difficulties, is still rudimentary. AIM The aim of this study was to evaluate the cumulative incidence rate and longitudinal course of orgasmic difficulties from age 30 to age 50, and the role of psychopathological and personality covariates in a representative population cohort of women. METHODS Semi-structured interviews with single-item questions on sexual problems in general as well as on orgasmic difficulties were conducted. Psychopathological covariates were from the Symptom Checklist 90 Revised and personality characteristics from the Freiburg Personality Inventory. Coping resources were measured by the scales of mastery and self-esteem. MAIN OUTCOME MEASURES One-year prevalences of orgasmic difficulties, associations with psychopathological and personality covariates as well as coping resources were the main outcome measures. RESULTS Orgasmic difficulties were reported annually by 7.4% to 13.5% of the women with a mean of 10.0% and a cumulative risk of 27.3% from age 30 to 50. No relation between age and the prevalence of orgasmic difficulties could be demonstrated. Psychopathological covariates such as depression, psychoticism, interpersonal sensitivity, obsessive-compulsive symptoms, and somatization were moderately associated with orgasmic difficulties. Also, personality traits, i.e., nervousness, aggressiveness, depressiveness, irritability, sociability, and openness, were related to orgasmic difficulties. CONCLUSIONS With a cumulative risk of 27.3%, orgasmic difficulties represent a frequent sexual problem. Annual prevalence rates are particularly high in women with psychopathological characteristics and modestly related to various personality traits. Our findings support the relevance of psychosocial factors in the regulation of female orgasm. More basic research is needed to better understand underlying pathophysiological mechanisms and to improve diagnostic as well as therapeutic tools for women suffering from orgasmic difficulties. https://sonar.ch/global/documents/12603 eng info:eu-repo/semantics/altIdentifier/doi/10.1111/jsm.12709 info:eu-repo/semantics/altIdentifier/pmid/25358802 The journal of sexual medicine. - 2014 Age Coping Resources Female Sexual Dysfunction Longitudinal Course Orgasmic Difficulties Personality Traits Psychopathology Adult Anxiety Cohort Studies Depression Emotions Female Humans Longitudinal Studies Middle Aged Orgasm Personality Personality Disorders Prevalence Prospective Studies Self Concept Sexual Dysfunctions, Psychological Women's Health The role of psychopathological and personality covariates in orgasmic difficulties: a prospective longitudinal evaluation in a cohort of women from age 30 to 50. http://purl.org/coar/resource_type/c_6501

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Merki-Feld GS Skouby S Serfaty D Lech M Bitzer J Crosignani PG Cagnacci A Sitruk-Ware R 2015 The obesity 'epidemic' continues to increase, mostly but not only in developed countries. As overweight and obese women are at an increased risk for venous thromboembolism (VTE) at baseline and at a much higher risk during pregnancy, it is essential to help these women to plan pregnancies carefully and to use contraceptives with a positive ratio of benefits versus risks. The Expert Group on hormonal and molecular contraception of the European Society of Contraception convened to review the existing evidence and propose recommendations to the prescribers in line with most recent studies and with the Medical Eligibility Criteria of the World Health Organisation. https://sonar.ch/global/documents/12606 eng info:eu-repo/semantics/altIdentifier/doi/10.3109/13625187.2014.960561 info:eu-repo/semantics/altIdentifier/pmid/25380138 The European journal of contraception & reproductive health care : the official journal of the European Society of Contraception. - 2015 Contraceptive failure Depo-medroxyprogesterone Efficacy Hormonal contraception Obesity Progestin-only contraception Thromboembolic risk Weight Contraception Contraception, Postcoital Contraceptive Agents, Female Developed Countries Europe Female Humans Intrauterine Devices, Medicated Obesity Overweight Pregnancy Societies, Medical Venous Thromboembolism European society of contraception statement on contraception in obese women. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12617 2025-10-24T20:48:26Z

Sturm V Hejcmanova M Landau K 2014 BACKGROUND Monocular infantile blindness may be associated with bilateral horizontal nystagmus, a subtype of fusion maldevelopment nystagmus syndrome (FMNS). Patients often adopt a significant anomalous head posture (AHP) towards the fixing eye in order to dampen the nystagmus. This clinical entity has also been reported as unilateral Ciancia syndrome. The aim of the study was to ascertain the clinical features and surgical outcome of patients with FMNS with infantile unilateral visual loss. METHODS In this retrospective case series, nine consecutive patients with FMNS with infantile unilateral visual loss underwent strabismus surgery to correct an AHP and/or improve ocular alignment. Outcome measures included amount of AHP and deviation at last follow-up. RESULTS Eye muscle surgery according to the principles of Kestenbaum resulted in a marked reduction or elimination of the AHP. On average, a reduction of AHP of 1.3°/mm was achieved by predominantly performing combined horizontal recess-resect surgery in the intact eye. In cases of existing esotropia (ET) this procedure also markedly reduced the angle of deviation. A dosage calculation of 3 prism diopters/mm was established. CONCLUSIONS We advocate a tailored surgical approach in FMNS with infantile unilateral visual loss. In typical patients who adopt a significant AHP accompanied by a large ET, we suggest an initial combined recess-resect surgery in the intact eye. This procedure regularly led to a marked reduction of the head turn and ET. In patients without significant strabismus, a full Kestenbaum procedure was successful, while ET in a patient with a minor AHP was corrected by performing a bimedial recession. application/pdf https://sonar.ch/global/documents/12617 eng info:eu-repo/semantics/altIdentifier/doi/10.1186/1471-2415-14-137 info:eu-repo/semantics/altIdentifier/pmid/25413480 info:eu-repo/semantics/openAccess BMC ophthalmology. - 2014 Adolescent Blindness Child Child, Preschool Female Humans Male Nystagmus, Pathologic Oculomotor Muscles Ophthalmologic Surgical Procedures Posture Retrospective Studies Strabismus Vision, Monocular Visual Acuity Effects of extraocular muscle surgery in children with monocular blindness and bilateral nystagmus. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12687 2025-10-24T20:48:29Z

Abdelhady W Chen L Bayer AS Seidl K Yeaman MR Kreiswirth BN Xiong YQ 2015 OBJECTIVES Persistent MRSA infections are especially relevant to endovascular infections and correlate with suboptimal outcomes. However, the virulence signatures of Staphylococcus aureus that drive such persistence outcomes are not well defined. In the current study, we investigated correlations between accessory gene regulator (agr) activation and the outcome of vancomycin treatment in an experimental model of infective endocarditis (IE) due to MRSA strains with different agr and clonal complex (CC) types. METHODS Twelve isolates with the four most common MRSA CC and agr types (CC5-agr II, CC8-agr I, CC30-agr III and CC45-agr I) were evaluated for heterogeneous vancomycin-intermediate S. aureus (hVISA), agr function, agrA and RNAIII transcription, agr locus sequences, virulence and response to vancomycin in the IE model. RESULTS Early agr RNAIII activation (beginning at 2 h of growth) in parallel with strong δ-haemolysin production correlated with persistent outcomes in the IE model following vancomycin therapy. Importantly, such treatment failures occurred across the range of CC/agr types studied. In addition, these MRSA strains: (i) were vancomycin susceptible in vitro; (ii) were not hVISA or vancomycin tolerant; and (iii) did not evolve hVISA phenotypes or perturbed δ-haemolysin activity in vivo following vancomycin therapy. Moreover, agr locus sequence analyses revealed no common point mutations that correlated with either temporal RNAIII transcription or vancomycin treatment outcomes, encompassing different CC and agr types. CONCLUSIONS These data suggest that temporal agr RNAIII activation and agr functional profiles may be useful biomarkers to predict the in vivo persistence of endovascular MRSA infections despite vancomycin therapy. application/pdf https://sonar.ch/global/documents/12687 eng info:eu-repo/semantics/altIdentifier/doi/10.1093/jac/dku547 info:eu-repo/semantics/altIdentifier/pmid/25564565 info:eu-repo/semantics/openAccess The Journal of antimicrobial chemotherapy. - 2015 antibiotics bacteria resistance Animals Anti-Bacterial Agents Bacterial Proteins Disease Models, Animal Endocarditis Gene Expression Profiling Methicillin-Resistant Staphylococcus aureus Rabbits Sequence Analysis, DNA Staphylococcal Infections Trans-Activators Transcriptional Activation Treatment Failure Virulence Early agr activation correlates with vancomycin treatment failure in multi-clonotype MRSA endovascular infections. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12693 2025-10-24T20:48:29Z

Mallano T Palumbo-Zerr K Zerr P Ramming A Zeller B Beyer C Dees C Huang J Hai T Distler O Schett G Distler JH 2016 BACKGROUND Activating transcription factor 3 (ATF3), a member of the ATF/cAMP-responsive element binding (CREB) family of transcription factors, regulates cellular response to stress including oxidative stress. The aim of this study was to analyse the role of ATF3 in fibroblast activation in systemic sclerosis (SSc). METHODS ATF3 was analysed by reverse transcription quantitative PCR, western blot and immunohistochemistry. ATF3 knockout fibroblasts and mice were used to study the functional role of ATF3. Knockdown experiments, reporter assays and coimmunoprecipitation were performed to study the effects of ATF3 on Smad and activation protein 1 (AP-1) signalling. The role of c-Jun was analysed by costaining, specific inactivation and coimmunoprecipitation. RESULTS Transforming growth factor-β (TGFβ) upregulates the expression of ATF3 in SSc fibroblasts. ATF3-deficient fibroblasts were less sensitive to TGFβ, whereas ectopic expression of ATF3 enhanced the profibrotic effects of TGFβ. Mechanistically, ATF3 interacts with Smad3 directly on stimulation with TGFβ and regulates Smad activity in a c-Jun-dependent manner. Knockout of ATF3 protected mice from bleomycin-induced fibrosis and fibrosis induced by overexpression of a constitutively active TGFβ receptor I. Reporter assays and analyses of the expression of Smad target genes demonstrated that binding of ATF3 regulates the transcriptional activity of Smad3. CONCLUSIONS We demonstrate for the first time a key role for ATF3 in fibrosis. Knockout of the ATF3 gene reduced the stimulatory effect of TGFβ on fibroblasts by interfering with canonical Smad signalling and protected the mice from experimental fibrosis in two different models. ATF3 might thus be a candidate for molecular targeted therapies for SSc. application/pdf https://sonar.ch/global/documents/12693 eng info:eu-repo/semantics/altIdentifier/doi/10.1136/annrheumdis-2014-206214 info:eu-repo/semantics/altIdentifier/pmid/25589515 info:eu-repo/semantics/openAccess Annals of the rheumatic diseases. - 2016 Fibroblasts Systemic Sclerosis Treatment Activating Transcription Factor 3 Adult Aged Animals Blotting, Western Case-Control Studies Dermis Female Fibroblasts Fibrosis Fluorescent Antibody Technique Gene Expression Profiling Gene Expression Regulation Humans Immunohistochemistry Male Mice Mice, Knockout Middle Aged Protein-Serine-Threonine Kinases Proto-Oncogene Proteins c-jun Receptor, Transforming Growth Factor-beta Type I Receptors, Transforming Growth Factor beta Reverse Transcriptase Polymerase Chain Reaction Scleroderma, Systemic Signal Transduction Smad3 Protein Transcription Factor AP-1 Transforming Growth Factor beta Young Adult Activating transcription factor 3 regulates canonical TGFβ signalling in systemic sclerosis. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12755 2025-10-24T20:48:34Z

Felber M Bauwens M Mateos JM Imstepf S Mottaghy FM Alberto R 2015 Radiolabeling allows noninvasive imaging by single photon emission computed tomography (SPECT) or positron emission tomography (PET) for assessing the biodistribution of nanostructures. Herein, the synthesis of a new coating ligand for gold nanoparticles (AuNPs) and quantum dots (QDs) is reported. This ligand is multifunctional; it combines the metal chelate with conjugating functions to biological vectors. The concept allows the coupling of any targeting function to the chelator; an example for the prostate specific membrane antigen is given. Derivatized NPs can directly be labeled in one step with [(99m) Tc(OH2 )3 (CO)3 ](+) . AuNPs in particular are highly stable, a prerequisite for in vivo studies excluding misinterpretation of the biodistribution data. AuNPs with differing sizes (7 and 14 nm core diameter) were administered intravenously into nude NMRI mice bearing LNCaP xenografts. MicroSPECT images show for both probes rapid clearance from the blood pool through the hepatobiliary pathway. The 7 nm AuNPs revealed a significantly higher bone uptake than the 14 nm AuNPs. The high affinity towards bone mineral is further confirmed in vitro with hydroxyapatite. https://sonar.ch/global/documents/12755 eng info:eu-repo/semantics/altIdentifier/doi/10.1002/chem.201405704 info:eu-repo/semantics/altIdentifier/pmid/25765900 Chemistry (Weinheim an der Bergstrasse, Germany). - 2015 gold nanoparticles radiochemistry radiolabeling technetium Animals COS Cells Cell Line Chlorocebus aethiops Gold Ligands Metal Nanoparticles Mice, Nude Models, Molecular Neoplasms Organotechnetium Compounds Quantum Dots Tissue Distribution Tomography, Emission-Computed, Single-Photon (99m) Tc radiolabeling and biological evaluation of nanoparticles functionalized with a versatile coating ligand. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12786 2025-10-24T20:48:36Z

Szövényi P Frangedakis E Ricca M Quandt D Wicke S Langdale JA 2015 BACKGROUND Plants colonized terrestrial environments approximately 480 million years ago and have contributed significantly to the diversification of life on Earth. Phylogenetic analyses position a subset of charophyte algae as the sister group to land plants, and distinguish two land plant groups that diverged around 450 million years ago - the bryophytes and the vascular plants. Relationships between liverworts, mosses hornworts and vascular plants have proven difficult to resolve, and as such it is not clear which bryophyte lineage is the sister group to all other land plants and which is the sister to vascular plants. The lack of comparative molecular studies in representatives of all three lineages exacerbates this uncertainty. Such comparisons can be made between mosses and liverworts because representative model organisms are well established in these two bryophyte lineages. To date, however, a model hornwort species has not been available. RESULTS Here we report the establishment of Anthoceros agrestis as a model hornwort species for laboratory experiments. Axenic culture conditions for maintenance and vegetative propagation have been determined, and treatments for the induction of sexual reproduction and sporophyte development have been established. In addition, protocols have been developed for the extraction of DNA and RNA that is of a quality suitable for molecular analyses. Analysis of haploid-derived genome sequence data of two A. agrestis isolates revealed single nucleotide polymorphisms at multiple loci, and thus these two strains are suitable starting material for classical genetic and mapping experiments. CONCLUSIONS Methods and resources have been developed to enable A. agrestis to be used as a model species for developmental, molecular, genomic, and genetic studies. This advance provides an unprecedented opportunity to investigate the biology of hornworts. application/pdf https://sonar.ch/global/documents/12786 eng info:eu-repo/semantics/altIdentifier/doi/10.1186/s12870-015-0481-x info:eu-repo/semantics/altIdentifier/pmid/25886741 info:eu-repo/semantics/openAccess BMC plant biology. - 2015 Anthocerotophyta Axenic Culture Molecular Sequence Data Sequence Analysis, DNA Establishment of Anthoceros agrestis as a model species for studying the biology of hornworts. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12620 2025-10-24T20:48:37Z

Gerber PA Berneis K 2012 PURPOSE OF REVIEW This article aims at reviewing the recent findings that have been made concerning the crosstalk of carbohydrate metabolism with the generation of small, dense low-density lipoprotein (LDL) particles, which are known to be associated with an adverse cardiovascular risk profile. RECENT FINDINGS Studies conducted during the past few years have quite unanimously shown that the quantity of carbohydrates ingested is associated with a decrease of LDL particle size and an increase in its density. Conversely, diets that aim at a reduction of carbohydrate intake are able to improve LDL quality. Furthermore, a reduction of the glycaemic index without changing the amount of carbohydrates ingested has similar effects. Diseases with altered carbohydrate metabolism, for example, type 2 diabetes, are associated with small, dense LDL particles. Finally, even the kind of monosaccharide the carbohydrate intake consists of is important concerning LDL particle size: fructose has been shown to alter the LDL particle subclass profile more adversely than glucose in many recent studies. SUMMARY LDL particle quality, rather than its quantity, is affected by carbohydrate metabolism, which is of clinical importance, in particular, in the light of increased carbohydrate consumption in today's world. application/pdf https://sonar.ch/global/documents/12620 eng info:eu-repo/semantics/altIdentifier/doi/10.1097/MCO.0b013e3283545a6d info:eu-repo/semantics/altIdentifier/pmid/22617560 info:eu-repo/semantics/openAccess Current opinion in clinical nutrition and metabolic care. - 2012 Blood Glucose Cardiovascular Diseases Diet Diet, Carbohydrate-Restricted Dietary Carbohydrates Energy Intake Glycemic Index Humans Lipoproteins, LDL Metabolic Diseases Monosaccharides Particle Size Regulation of low-density lipoprotein subfractions by carbohydrates. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12707 2025-10-24T20:48:42Z

Eichholzer M Steinbrecher A Kaaks R Teucher B Linseisen J Rohrmann S 2012 UNLABELLED Study Type--Prognosis (case control) Level of Evidence 2. What's known on the subject? and What does the study add? Geographical and ethnic differences in the distribution of BPH and the results of migrant studies indicate that not only age, androgens and genetics, but also modifiable factors may play a role in the aetiology of BPH. Oxidative stress induced by chronic inflammation could be a cause and antioxidants, including selenoproteins, may reduce the risk. The published data related to this topic are scarce and are mainly based on cross-sectional and case-control studies. In a nested case-control study, we observed a significant inverse association between serum selenium concentrations and the risk of BPH. These results need to be confirmed in larger, prospective epidemiological studies. Prostate enlargement is an increasing health problem as a result of an ageing population in many countries. Modifiable factors may also play a role. In the present study, before this antioxidant can be recommended as a preventive measure. OBJECTIVE • To determine whether geographical differences in the distribution of benign prostatic hyperplasia (BPH) and migrant studies indicate that modifiable factors play a role in the aetiology of BPH. Oxidative stress produced by chronic inflammation could represent one of the causes, and antioxidants, including selenoproteins, may reduce the risk. SUBJECTS AND METHODS • Conditional logistic regression was used to examine the associations of serum selenium and selenoprotein P concentrations and glutathione peroxidase activity with respect to the risk of BPH in a case-control study nested in the European Prospective Investigation into Cancer and Nutrition-Heidelberg cohort, including 111 cases and 214 matched controls. • In addition, dietary glucosinolate intake and the serum glutathione S-transferase α concentration was investigated. RESULTS • The risk of BPH significantly decreased with an increasing serum selenium concentration; the risk estimate was 0.83 (35% CI 0.69-0.99) per 10 µg/L increase in serum selenium concentration. • However, no significant association was present for serum selenoprotein P concentration or glutathione peroxidase activity. Risk estimates for BPH decreased with a higher intake of glucosinolates, although the results were not statistically significant. CONCLUSION • A low serum selenium concentration may increase the risk of BPH, although the findings reported in the present study need to be confirmed in larger, well-designed epidemiological studies. https://sonar.ch/global/documents/12707 eng info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1464-410X.2012.11383.x info:eu-repo/semantics/altIdentifier/pmid/22882569 BJU international. - 2012 Adult Aged Biomarkers Dietary Supplements Follow-Up Studies Germany Glucosinolates Glutathione Transferase Humans Incidence Isoenzymes Male Middle Aged Prostatic Hyperplasia Retrospective Studies Risk Factors Selenium Effects of selenium status, dietary glucosinolate intake and serum glutathione S-transferase α activity on the risk of benign prostatic hyperplasia. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12729 2025-10-24T20:48:46Z

Wulf MA Bode B Zimmermann D Rufibach K Weder W Moch H Soltermann A Tischler V 2012 Epidermal growth factor receptor (EGFR) gene mutation and high gene copy number (CN) predict response to EGFR tyrosine kinase inhibitor therapy in the adenocarcinoma subtype of non-small cell lung cancer (NSCLC). The aims of this study were first to compare automated enzyme metallographic silver-enhanced in situ hybridization (SISH) with conventionally used fluorescence in situ hybridization (FISH) in the determination of EGFR CN in NSCLC tissue sections, and second to assess the association of EGFR CN with EGFR mutations and clinicopathological parameters. FISH and SISH were performed on tissue microarrays and large sections. Samples from 56 consecutively surgically resected NSCLC patients (cohort 1) and from 60 selected lung adenocarcinoma patients (cohort 2) were analyzed. EGFR CN was classified applying the Colorado criteria, and agreement between both methods was evaluated using κ values. EGFR CN was compared with EGFR protein expression and EGFR gene mutations. The results of SISH and FISH were identical in 114 of the 116 cases examined using the 2 techniques. One case was FISH+, SISH-, and 1 case was FISH- and SISH+. The agreement between the 2 methods was good in cohort 1 (κ=0.642 [0.428, 0.823]) and excellent in cohort 2 (κ=0.963 [0.870, 1.000]). EGFR positivity by FISH and SISH was associated with high EGFR protein expression (P<0.001) and EGFR mutation (P<0.001). These results validate the use of SISH for assessing EGFR CN alterations in NSCLC. The advantage of a permanent result and the possibility of bright-field microscopy make SISH an attractive alternative to FISH. https://sonar.ch/global/documents/12729 eng info:eu-repo/semantics/altIdentifier/doi/10.1097/PAS.0b013e31826ff153 info:eu-repo/semantics/altIdentifier/pmid/23154768 The American journal of surgical pathology. - 2012 Acetates Aged Automation, Laboratory Biomarkers, Tumor Carcinoma, Non-Small-Cell Lung DNA Copy Number Variations DNA Mutational Analysis ErbB Receptors Female Gene Dosage Humans Immunohistochemistry In Situ Hybridization In Situ Hybridization, Fluorescence Lung Neoplasms Male Middle Aged Mutation Predictive Value of Tests Reproducibility of Results Silver Compounds Tissue Array Analysis Silver-enhanced in situ hybridization for determination of EGFR copy number alterations in non-small cell lung cancer. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12752 2025-10-24T20:48:47Z

Jäncke L Rogenmoser L Meyer M Elmer S 2012 BACKGROUND Coloured-hearing (CH) synesthesia is a perceptual phenomenon in which an acoustic stimulus (the inducer) initiates a concurrent colour perception (the concurrent). Individuals with CH synesthesia "see" colours when hearing tones, words, or music; this specific phenomenon suggesting a close relationship between auditory and visual representations. To date, it is still unknown whether the perception of colours is associated with a modulation of brain functions in the inducing brain area, namely in the auditory-related cortex and associated brain areas. In addition, there is an on-going debate as to whether attention to the inducer is necessarily required for eliciting a visual concurrent, or whether the latter can emerge in a pre-attentive fashion. RESULTS By using the EEG technique in the context of a pre-attentive mismatch negativity (MMN) paradigm, we show that the binding of tones and colours in CH synesthetes is associated with increased MMN amplitudes in response to deviant tones supposed to induce novel concurrent colour perceptions. Most notably, the increased MMN amplitudes we revealed in the CH synesthetes were associated with stronger intracerebral current densities originating from the auditory cortex, parietal cortex, and ventral visual areas. CONCLUSIONS The automatic binding of tones and colours in CH synesthetes is accompanied by an early pre-attentive process recruiting the auditory cortex, inferior and superior parietal lobules, as well as ventral occipital areas. application/pdf https://sonar.ch/global/documents/12752 eng info:eu-repo/semantics/altIdentifier/doi/10.1186/1471-2202-13-151 info:eu-repo/semantics/altIdentifier/pmid/23241212 info:eu-repo/semantics/openAccess BMC neuroscience. - 2012 Acoustic Stimulation Adult Attention Auditory Cortex Auditory Perception Brain Waves Case-Control Studies Color Perception Electroencephalography Female Humans Male Parietal Lobe Perceptual Disorders Synesthesia Visual Cortex Pre-attentive modulation of brain responses to tones in coloured-hearing synesthetes. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12616 2025-10-24T20:48:49Z

Burkhardt JK Burkhardt WF Zinn PO Bozinov O Bertalanffy H 2011 All medical subspecialties and, in particular, high-tech field neurosurgery are subject to continuous change in therapeutic concepts due to novel treatment options emerging through research and evolution in the field. Hence, the question arises if the patient's informed consent needs to be adjusted in the face of multiple therapeutic options with different configurations of risk/benefit relationships. In this paper we discuss different therapeutic scenarios with regard to ruptured intracerebral aneurysms (RIA) and unruptured intracerebral aneurysms (UIA), and we advise the medical doctor involved in the process. Indeed, experienced neurosurgeons and endovascular interventionalists are very familiar with the management of these scenarios; likewise, interns, residents, and otherwise affiliated physicians may be faced with these situations. In general, asymptomatic and mentally capacitated patients as well as legal guardians of minor patients and of mentally incapacitated patients with incidental aneurysms and more than one therapeutic option need to be accurately informed about the advantages and disadvantages of the treatment options available to them. In case of emergency or when treating a mentally disabled patient who is incapable of making a sound judgment and has no legal guardian, the attending doctor will have to choose the best possible treatment option. application/pdf https://sonar.ch/global/documents/12616 eng info:eu-repo/semantics/altIdentifier/doi/10.1016/j.zefq.2010.07.040 info:eu-repo/semantics/altIdentifier/pmid/21958620 info:eu-repo/semantics/openAccess Zeitschrift fur Evidenz, Fortbildung und Qualitat im Gesundheitswesen. - 2011 Aneurysm, Ruptured Germany Humans Informed Consent Intracranial Aneurysm Legal Guardians Malpractice Mental Competency National Health Programs Outcome and Process Assessment, Health Care Patient Education as Topic Prognosis Risk Assessment [Intracerebral aneurysm--treatment options, informed consent, and legal aspects]. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12621 2025-10-24T20:48:49Z

Staubli S 2011 This paper studies the effect of a large-scale policy change in the Austrian disability insurance program, which tightened eligibility criteria for men above a certain age. Using administrative data on the universe of Austrian private-sector employees, the results of difference-in-difference regressions suggest a substantial and statistically significant decline in disability enrollment of 6 to 7.4 percentage points and an increase in employment of 1.6 to 3.4 percentage points. The policy change had important spillover effects into the unemployment and sickness insurance program. Specifically, the share of individuals receiving unemployment benefits increased by 3.5 to 3.9 percentage points, and the share receiving sickness insurance benefits, by roughly 0.7 percentage points. https://sonar.ch/global/documents/12621 eng info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jpubeco.2011.05.008 info:eu-repo/semantics/altIdentifier/pmid/21969737 Journal of public economics. - 2011 The impact of stricter criteria for disability insurance on labor force participation. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12623 2025-10-24T20:48:49Z

Münz C 2011 Macroautophagy is a catabolic pathway in eukaryotic cells that has recently been shown to facilitate pathogen detection, pathogen restriction and pathogen-derived antigen presentation to CD4(+) T cells. Due to these protective functions during immune responses, several pathogens, including RNA and DNA viruses, have developed strategies to inhibit autophagosome generation or maturation. Interestingly, most of the respective viral proteins exert these functions via binding to Beclin-1, an essential macroautophagy protein that constitutes part of the phosphatidylinositol-3 kinase complexes that mark membranes for autophagosome generation and facilitate autophagosome fusion with lyososomes. The viruses that inhibit macroautophagy by this pathway include herpesviruses, HIV and influenza A virus. Inhibition either before or after autophagosome formation seems to benefit their viral replication by different mechanisms, which are discussed here. application/pdf https://sonar.ch/global/documents/12623 eng info:eu-repo/semantics/altIdentifier/doi/10.3390/v3071166 info:eu-repo/semantics/altIdentifier/pmid/21994775 info:eu-repo/semantics/openAccess Viruses. - 2011 HIV HSV KSHV autophagy immune evasion immunity influenza virus Animals Apoptosis Regulatory Proteins Autophagy Beclin-1 DNA Viruses Humans Immune Evasion Membrane Proteins RNA Viruses Virus Diseases Beclin-1 targeting for viral immune escape. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12632 2025-10-24T20:48:51Z

Schweiger A Grimm F Tanner I Müllhaupt B Bertogg K Müller N Deplazes P 2012 PURPOSE Human alveolar (AE) and cystic echinococcosis (CE) caused by the metacestode stages of Echinococcus multilocularis and E. granulosus, respectively, lack pathognomonic clinical signs. Diagnosis therefore relies on the results of imaging and serological studies. The primary goal of this study was to evaluate the efficacy of several easy-to-produce crude or partially purified E. granulosus and E. multilocularis metacestode-derived antigens as tools for the serological diagnosis and differential diagnosis of patients suspicious for AE or CE. METHODS The sera of 51 treatment-naïve AE and 32 CE patients, 98 Swiss blood donors and 38 patients who were initially suspicious for echinococcosis but suffering from various other liver diseases (e.g., liver neoplasia, etc.) were analysed. RESULTS According to the results of enzyme-linked immunosorbent assays (ELISA), metacestode-derived antigens of E. granulosus had sensitivities varying from 81 to 97% and >99.9% for the diagnosis of CE and AE, respectively. Antigens derived from E. multilocularis metacestodes had sensitivities ranging from 84 to 91% and >99.9% for the diagnosis of CE and AE, respectively. Specificities ranged from 92 to >99.9%. Post-test probabilities for the differential diagnosis of AE from liver neoplasias, CE from cystic liver lesions, and screening for AE in Switzerland were around 95, 86 and 2.2%, respectively. Cross-reactions with antibodies in sera of patients with other parasitic affections (fasciolosis, schistosomosis, amebosis, cysticercosis, and filarioses) did occur at variable frequencies, but could be eliminated through the use of confirmatory testing. CONCLUSIONS Different metacestode-derived antigens of E. granulosus and E. multilocularis are valuable, widely accessible, and cost-efficient tools for the serological diagnosis of echinococcosis. However, confirmatory testing is necessary, due to the lack of species specificity and the occurrence of cross-reactions to other helminthic diseases. application/pdf https://sonar.ch/global/documents/12632 eng info:eu-repo/semantics/altIdentifier/doi/10.1007/s15010-011-0205-6 info:eu-repo/semantics/altIdentifier/pmid/22076692 info:eu-repo/semantics/openAccess Infection. - 2012 Adult Aged Animals Antibodies, Helminth Antigens, Helminth Cross Reactions Diagnosis, Differential Echinococcosis Echinococcosis, Hepatic Echinococcus granulosus Echinococcus multilocularis Enzyme-Linked Immunosorbent Assay Female Humans Male Middle Aged Switzerland Serological diagnosis of echinococcosis: the diagnostic potential of native antigens. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12652 2025-10-24T20:48:53Z

Kampa BM Roth MM Göbel W Helmchen F 2011 How are visual scenes encoded in local neural networks of visual cortex? In rodents, visual cortex lacks a columnar organization so that processing of diverse features from a spot in visual space could be performed locally by populations of neighboring neurons. To examine how complex visual scenes are represented by local microcircuits in mouse visual cortex we measured visually evoked responses of layer 2/3 neuronal populations using 3D two-photon calcium imaging. Both natural and artificial movie scenes (10 seconds duration) evoked distributed and sparsely organized responses in local populations of 70-150 neurons within the sampled volumes. About 50% of neurons showed calcium transients during visual scene presentation, of which about half displayed reliable temporal activation patterns. The majority of the reliably responding neurons were activated primarily by one of the four visual scenes applied. Consequently, single-neurons performed poorly in decoding, which visual scene had been presented. In contrast, high levels of decoding performance (>80%) were reached when considering population responses, requiring about 80 randomly picked cells or 20 reliable responders. Furthermore, reliable responding neurons tended to have neighbors sharing the same stimulus preference. Because of this local redundancy, it was beneficial for efficient scene decoding to read out activity from spatially distributed rather than locally clustered neurons. Our results suggest a population code in layer 2/3 of visual cortex, where the visual environment is dynamically represented in the activation of distinct functional sub-networks. application/pdf https://sonar.ch/global/documents/12652 eng info:eu-repo/semantics/altIdentifier/doi/10.3389/fncir.2011.00018 info:eu-repo/semantics/altIdentifier/pmid/22180739 info:eu-repo/semantics/openAccess Frontiers in neural circuits. - 2011 3D imaging calcium imaging natural movies neocortex two-photon microscopy Representation of visual scenes by local neuronal populations in layer 2/3 of mouse visual cortex. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12660 2025-10-24T20:48:58Z

Scharl M Frei P Frei SM Biedermann L Weber A Rogler G 2014 Anal adenocarcinomas arising from perianal fistulae represent a rare complication in Crohn's disease (CD) patients. We have previously demonstrated the involvement of an epithelial-to-mesenchymal transition (EMT) in the pathogenesis of CD-associated fistulae. Although EMT has also been implicated in the development of colorectal and anal carcinoma, the molecular link from fistula to carcinoma is unclear. We present a case of a 48-year-old White woman who developed a mucinous anal adenocarcinoma originating from a perianal, CD-associated fistula 24 years after being diagnosed with CD. To characterize the expression of EMT-associated molecules in fistula and carcinoma tissue, immunohistochemical analysis for Snail1, Slug, β-catenin and E-cadherin was performed. A mucinous anal adenocarcinoma developed on a perianal fistula in a patient with long-standing CD. After neoadjuvant radiochemotherapy, the fistula-associated tumour was resected and the patient is presently in remission. Using immunohistochemical analysis, we detected a remarkable staining of the Slug transcription factor in transitional cells lining the fistula tract. This observation is unique to this 'carcinoma'-fistula: we had previously shown Slug expression in cells surrounding the fistula tract but not in transitional cells. Expression of Snail1, β-catenin and E-cadherin in this case was comparable with our previous findings. We describe a rare case of a CD fistula-associated adenocarcinoma within an area of squamous epithelium of the perianal area and an unusual expression pattern of EMT markers in this fistula. This case seems to underline the relevance of our previous findings demonstrating that EMT plays an important role for fistula pathogenesis and likely carcinogenesis in CD patients. application/pdf https://sonar.ch/global/documents/12660 eng info:eu-repo/semantics/altIdentifier/doi/10.1097/MEG.0b013e32836371a2 info:eu-repo/semantics/altIdentifier/pmid/24284372 info:eu-repo/semantics/openAccess European journal of gastroenterology & hepatology. - 2014 Adenocarcinoma, Mucinous Antigens, CD Anus Neoplasms Biomarkers, Tumor Cadherins Chemoradiotherapy, Adjuvant Colonoscopy Crohn Disease Endosonography Epithelial-Mesenchymal Transition Female Humans Magnetic Resonance Imaging Middle Aged Neoadjuvant Therapy Rectal Fistula Snail Family Transcription Factors Transcription Factors Treatment Outcome beta Catenin Epithelial-to-mesenchymal transition in a fistula-associated anal adenocarcinoma in a patient with long-standing Crohn's disease. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12686 2025-10-24T20:49:02Z

Roesslein M Froehlich C Jans F Piegeler T Goebel U Loop T 2014 AIMS Dobutamine is cytoprotective when applied before a subsequent stress. However, the underlying molecular mechanism is unknown. Dobutamine also inhibits nuclear factor (NF)-κB in human T lymphocytes. Other inhibitors of NF-κB induce a so-called heat shock response. We hypothesized that dobutamine mediates protection from apoptotic cell death by the induction of a heat shock response. MAIN METHODS Jurkat T lymphoma cells were preincubated with dobutamine (0.1, 0.5 mM) before the induction of apoptosis (staurosporine, 2 μM). DNA-binding of heat shock factor (HSF)-1 was analyzed by electrophoretic mobility shift assay, mRNA-expression of heat shock protein (hsp)70 and hsp90 by Northern Blot, activity of caspase-3 by fluorogenic caspase activity assay and cleavage of pro-caspase-3 by Western Blot. Apoptosis was assessed by flow cytometry after annexin V-fluorescein isothiocyanate staining. Hsp70 and hsp90 were inhibited using N-formyl-3,4-methylenedioxy-benzylidene-gamma-butyrolaetam and 17-allylamino-17-demethoxygeldana-mycin, respectively. All data are given as median and 25/75% percentile. KEY FINDINGS Pre-incubation with dobutamine inhibited staurosporine-induced annexin V-fluorescence (28 [20-32] % vs. 12 [9-15] % for dobutamine 0.1 mM and 7 [5-12] % for dobutamine 0.5 mM, p<0.001), cleavage of pro-caspase-3 as well as caspase-3-like activity (0.46 [0.40-0.48] vs. 0.32 [0.27-0.39] for Dobutamine 0.1 mM and 0.20 [0.19-0.23] for Dobutamine 0.5 mM, p<0.01). Dobutamine induced DNA-binding of HSF-1 and mRNA-expression of hsp70 and hsp90. While inhibition of Hsp90 had no effect, inhibition of Hsp70 increased the number of annexin V-positive cells (33 [32-36] % vs. 18 [16-24] %) and caspase-3-like activity (0.21 [0.19-0.23] vs. 0.16 [0.13-0.17], p<0.05). SIGNIFICANCE Dobutamine protects from apoptotic cell death via the induction of Hsp70. https://sonar.ch/global/documents/12686 eng info:eu-repo/semantics/altIdentifier/doi/10.1016/j.lfs.2014.01.005 info:eu-repo/semantics/altIdentifier/pmid/24447628 Life sciences. - 2014 Apoptosis Dobutamine Dobutamine hydrochloride (PubChem CID: 65324) Heat shock protein 70 Heat shock response Protection Adrenergic beta-1 Receptor Agonists Apoptosis Blotting, Northern Cell Line, Tumor Cytoprotection Dobutamine Electrophoresis, Polyacrylamide Gel Flow Cytometry Gene Expression Regulation HSP70 Heat-Shock Proteins Humans Protein-Serine-Threonine Kinases RNA, Messenger Real-Time Polymerase Chain Reaction Dobutamine mediates cytoprotection by induction of heat shock protein 70 in vitro. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12732 2025-10-24T20:49:06Z

Beyer C Huang J Beer J Zhang Y Palumbo-Zerr K Zerr P Distler A Dees C Maier C Munoz L Krönke G Uderhardt S Distler O Jones S Rose-John S Oravecz T Schett G Distler JH 2015 OBJECTIVES To investigate the role of liver X receptors (LXRs) in experimental skin fibrosis and evaluate their potential as novel antifibrotic targets. METHODS We studied the role of LXRs in bleomycin-induced skin fibrosis, in the model of sclerodermatous graft-versus-host disease (sclGvHD) and in tight skin-1 (Tsk-1) mice, reflecting different subtypes of fibrotic disease. We examined both LXR isoforms using LXRα-, LXRβ- and LXR-α/β-double-knockout mice. Finally, we investigated the effects of LXRs on fibroblasts and macrophages to establish the antifibrotic mode of action of LXRs. RESULTS LXR activation by the agonist T0901317 had antifibrotic effects in bleomycin-induced skin fibrosis, in the sclGvHD model and in Tsk-1 mice. The antifibrotic activity of LXRs was particularly prominent in the inflammation-driven bleomycin and sclGvHD models. LXRα-, LXRβ- and LXRα/β-double-knockout mice showed a similar response to bleomycin as wildtype animals. Low levels of the LXR target gene ABCA-1 in the skin of bleomycin-challenged and control mice suggested a low baseline activation of the antifibrotic LXR signalling, which, however, could be specifically activated by T0901317. Fibroblasts were not the direct target cells of LXRs agonists, but LXR activation inhibited fibrosis by interfering with infiltration of macrophages and their release of the pro-fibrotic interleukin-6. CONCLUSIONS We identified LXRs as novel targets for antifibrotic therapies, a yet unknown aspect of these nuclear receptors. Our data suggest that LXR activation might be particularly effective in patients with inflammatory disease subtypes. Activation of LXRs interfered with the release of interleukin-6 from macrophages and, thus, inhibited fibroblast activation and collagen release. application/pdf https://sonar.ch/global/documents/12732 eng info:eu-repo/semantics/altIdentifier/doi/10.1136/annrheumdis-2013-204401 info:eu-repo/semantics/altIdentifier/pmid/24618263 info:eu-repo/semantics/openAccess Annals of the rheumatic diseases. - 2015 Fibroblasts Systemic Sclerosis Treatment Animals Antibiotics, Antineoplastic Bleomycin Disease Models, Animal Fibroblasts Fibrosis Humans Hydrocarbons, Fluorinated Interleukin-6 Liver X Receptors Macrophages Mice Mice, Knockout Orphan Nuclear Receptors Scleroderma, Diffuse Skin Skin Diseases Sulfonamides Activation of liver X receptors inhibits experimental fibrosis by interfering with interleukin-6 release from macrophages. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12741 2025-10-24T20:49:07Z

Maier PJ Zemoura K Acuña MA Yévenes GE Zeilhofer HU Benke D 2014 Cerebral ischemia frequently leads to long-term disability and death. Excitotoxicity is believed to be the main cause for ischemia-induced neuronal death. Although a role of glutamate receptors in this process has been firmly established, the contribution of metabotropic GABAB receptors, which control excitatory neurotransmission, is less clear. A prominent characteristic of ischemic insults is endoplasmic reticulum (ER) stress associated with the up-regulation of the transcription factor CCAAT/enhancer-binding protein-homologous protein (CHOP). After inducing ER stress in cultured cortical neurons by sustained Ca(2+) release from intracellular stores or by a brief episode of oxygen and glucose deprivation (in vitro model of cerebral ischemia), we observed an increased expression of CHOP accompanied by a strong reduction of cell surface GABAB receptors. Our results indicate that down-regulation of cell surface GABAB receptors is caused by the interaction of the receptors with CHOP in the ER. Binding of CHOP prevented heterodimerization of the receptor subunits GABAB1 and GABAB2 and subsequent forward trafficking of the receptors to the cell surface. The reduced level of cell surface receptors diminished GABAB receptor signaling and, thus, neuronal inhibition. These findings indicate that ischemia-mediated up-regulation of CHOP down-regulates cell surface GABAB receptors by preventing their trafficking from the ER to the plasma membrane. This mechanism leads to diminished neuronal inhibition and may contribute to excitotoxicity in cerebral ischemia. application/pdf https://sonar.ch/global/documents/12741 eng info:eu-repo/semantics/altIdentifier/doi/10.1074/jbc.M114.550517 info:eu-repo/semantics/altIdentifier/pmid/24668805 info:eu-repo/semantics/openAccess The Journal of biological chemistry. - 2014 CHOP Cell Surface Cerebral Ischemia Endoplasmic Reticulum (ER) Endoplasmic Reticulum Stress G Protein-coupled Receptors (GPCR) GABA Receptors Neurons Trafficking Animals Brain Ischemia Cell Membrane Cells, Cultured Down-Regulation Endoplasmic Reticulum Stress Female Gene Expression Glucose HEK293 Cells Humans Microscopy, Confocal Neurons Oxygen Protein Binding Rats Rats, Wistar Receptors, GABA-B Reverse Transcriptase Polymerase Chain Reaction Transcription Factor CHOP Ischemia-like oxygen and glucose deprivation mediates down-regulation of cell surface γ-aminobutyric acidB receptors via the endoplasmic reticulum (ER) stress-induced transcription factor CCAAT/enhancer-binding protein (C/EBP)-homologous protein (CHOP). http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12777 2025-10-24T20:49:10Z

Csomor PA Preller KH Geyer MA Studerus E Huber T Vollenweider FX 2014 Despite advances in the treatment of schizophrenia spectrum disorders with atypical antipsychotics (AAPs), there is still need for compounds with improved efficacy/side-effect ratios. Evidence from challenge studies suggests that the assessment of gating functions in humans and rodents with naturally low-gating levels might be a useful model to screen for novel compounds with antipsychotic properties. To further evaluate and extend this translational approach, three AAPs were examined. Compounds without antipsychotic properties served as negative control treatments. In a placebo-controlled, within-subject design, healthy males received either single doses of aripiprazole and risperidone (n=28), amisulpride and lorazepam (n=30), or modafinil and valproate (n=30), and placebo. Prepulse inhibiton (PPI) and P50 suppression were assessed. Clinically associated symptoms were evaluated using the SCL-90-R. Aripiprazole, risperidone, and amisulpride increased P50 suppression in low P50 gaters. Lorazepam, modafinil, and valproate did not influence P50 suppression in low gaters. Furthermore, low P50 gaters scored significantly higher on the SCL-90-R than high P50 gaters. Aripiprazole increased PPI in low PPI gaters, whereas modafinil and lorazepam attenuated PPI in both groups. Risperidone, amisulpride, and valproate did not influence PPI. P50 suppression in low gaters appears to be an antipsychotic-sensitive neurophysiologic marker. This conclusion is supported by the association of low P50 suppression and higher clinically associated scores. Furthermore, PPI might be sensitive for atypical mechanisms of antipsychotic medication. The translational model investigating differential effects of AAPs on gating in healthy subjects with naturally low gating can be beneficial for phase II/III development plans by providing additional information for critical decision making. application/pdf https://sonar.ch/global/documents/12777 eng info:eu-repo/semantics/altIdentifier/doi/10.1038/npp.2014.102 info:eu-repo/semantics/altIdentifier/pmid/24801767 info:eu-repo/semantics/openAccess Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology. - 2014 Acoustic Stimulation Amisulpride Antipsychotic Agents Aripiprazole Auditory Perception Benzhydryl Compounds Brain Double-Blind Method Electroencephalography Electromyography Humans Lorazepam Male Modafinil Piperazines Prepulse Inhibition Psychometrics Psychotropic Drugs Quinolones Risperidone Sensory Gating Sulpiride Valproic Acid Young Adult Influence of aripiprazole, risperidone, and amisulpride on sensory and sensorimotor gating in healthy 'low and high gating' humans and relation to psychometry. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12814 2025-10-24T20:49:11Z

Noll G Noll S Hürlimann D 2014 Atrial fibrillation (AF), the most frequent sustained arrhythmia, is associated with an increased risk of thromboembolic events. The risk of stroke depends on risk factors such as age, hypertension, heart failure, and vascular disease. Thus, antithrombotic therapy is a cornerstone in the management of AF. Warfarin is successfully used to reduce thromboembolic events. More recently, direct thrombin (dabigatran) and factor Xa (apixaban, edoxaban, rivaroxaban) inhibitors have been compared to warfarin in large randomized trials. All new substances have been shown to be non-inferior to warfarin concerning thromboembolic events. Severe bleeding, such as fatal and intracranial bleeding, was less frequent with direct oral anticoagulants. Results of the studies and subgroup analyses are discussed. Further trials using direct oral anticoagulants in special populations such as very old and patients with kidney disease are needed. https://sonar.ch/global/documents/12814 eng info:eu-repo/semantics/altIdentifier/doi/10.1053/j.seminhematol.2014.04.001 info:eu-repo/semantics/altIdentifier/pmid/24861798 Seminars in hematology. - 2014 Administration, Oral Anticoagulants Atrial Fibrillation Benzimidazoles Dabigatran Embolism Hemorrhage Humans Morpholines Pyrazoles Pyridines Pyridones Risk Factors Rivaroxaban Stroke Thiazoles Thiophenes Warfarin beta-Alanine Direct oral anticoagulants in atrial fibrillation. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12835 2025-10-24T20:49:13Z

Fuge P Aust S Fan Y Weigand A Gärtner M Feeser M Bajbouj M Grimm S 2014 BACKGROUND Early life stress (ELS) experience is associated with persisting working memory (WM) deficits; changes to the corticotropin-releasing hormone (CRH) system; and structural, functional, and epigenetic changes in the hippocampus. Single nucleotide polymorphisms in the CRH receptor 1 (CRHR1) gene interact with ELS experience to predict depression as well as neuroendocrine and neuronal reactivity. Although these findings indicate that vulnerable genotypes might also show impaired WM performance after ELS experience, no previous study investigated whether there is an interaction effect of CRHR1 polymorphisms and ELS experience on WM performance. METHODS Subjects (N = 451) were genotyped for rs110402 and rs242924 within the CRHR1 gene. We used an n-back task to investigate the hypothesis that WM performance in healthy subjects may be subtly influenced by functional differences in CRHR1 and represents an early marker of increased vulnerability after exposure to ELS. RESULTS Exposure to ELS had a particularly strong impact on WM performance in subjects with the common homozygous GG GG genotype, whereas only severe exposure to ELS interfered with WM accuracy in AT carriers. CONCLUSIONS Our data indicate that specific CRHR1 polymorphisms moderate the effect of ELS experience on WM performance. Exposure to ELS in combination with a vulnerable genotype results in subtle memory deficits in adulthood, which might develop before psychopathological symptoms. https://sonar.ch/global/documents/12835 eng info:eu-repo/semantics/altIdentifier/doi/10.1016/j.biopsych.2014.04.016 info:eu-repo/semantics/altIdentifier/pmid/24931706 Biological psychiatry. - 2014 Corticotropin-releasing hormone receptor 1 early life stress hippocampus rs110402 rs242924 working memory Adult Age Factors Female Genotype Humans Male Memory, Short-Term Middle Aged Polymorphism, Single Nucleotide Receptors, Corticotropin-Releasing Hormone Stress, Psychological Interaction of early life stress and corticotropin-releasing hormone receptor gene: effects on working memory. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12837 2025-10-24T20:49:13Z

Haas F Yamauchi K Murat M Bernasconi M Yamanaka N Speck RF Nadal D 2014 UNLABELLED In order to understand and possibly treat B-cell malignancies associated with latent gammaherpesvirus infection, it is vital to understand the factors that control the balance between the two transcriptional states of gammaherpesviruses: latency and lytic replication. We used murine gammaherpesvirus 68 (MHV 68) as a model system to investigate how engagement of endosomal Toll-like receptors (TLRs) impacts reactivation from latency in vitro and establishment of latent infection in vivo. We found that treatment with TLR7 ligand R848 or TLR9 ligand CpG oligodeoxynucleotide (ODN) suppresses reactivation of MHV 68 in vitro. These suppressive effects correlated with the ability to activate cellular transcription factor NF-κB. Downregulation of TLR9 by RNA interference in vitro led to a reduction of nuclear levels of NF-κB p65 and consequently to an increase of spontaneous reactivation in cells latently infected with MHV 68, indicating that the TLR9 pathway suppresses spontaneous reactivation events. In vivo, sustained stimulation of TLR7 by repeated R848 treatment led to an increased frequency of infected splenocytes compared to mock-treated control results. Frequencies of infected splenic B cells in tlr7-/- or tlr9-/- mice after establishment of latency did not differ from those seen with their wild-type counterparts. Nevertheless, MHV 68-infected B cells from tlr9-/- mice showed a higher frequency of reactivation than B cells from wild-type or tlr7-/- mice in ex vivo reactivation assays. Thus, we show a suppressive effect of TLR7 or TLR9 triggering on MHV 68 reactivation that correlates with NF-κB activation and that the mere presence of a functional TLR9 signaling pathway contributes to dampen lytic gammaherpesvirus reactivation in infected cells. IMPORTANCE A hallmark of gammaherpesviruses is their establishment of latency in B cells that is reversible through lytic reactivation. Latency can result in B-cell malignancies. Activation of the innate immune system is thought to contribute to controlling the switch between the transcriptional states of latency and reactivation. Nevertheless, the mechanisms involved are not clear. Here, we show that engagement of Toll-like receptor 7 (TLR7) and TLR9 suppresses reactivation of murine gammaherpesvirus MHV 68 in vitro and that stimulation of TLR7 in vivo increases the frequency of infected cells. TLR7 and TLR9 are innate immunity sensors of nucleic acids localized in endosomes. Additionally, we demonstrate that impairment of TLR9 signaling in latently infected B cells leads to increased reactivation. Thus, activated endosomal TLR7 and TLR9 pathways play an important role in promoting establishment of latent gammaherpesvirus infection. Counteracting signaling of these pathways allows reactivation and could represent treatment targets in gammaherpesvirus-associated malignancies. application/pdf https://sonar.ch/global/documents/12837 eng info:eu-repo/semantics/altIdentifier/doi/10.1128/JVI.01486-14 info:eu-repo/semantics/altIdentifier/pmid/24942583 info:eu-repo/semantics/openAccess Journal of virology. - 2014 Animals Herpesviridae Infections Membrane Glycoproteins Mice, Inbred C57BL NF-kappa B Rhadinovirus Toll-Like Receptor 7 Toll-Like Receptor 9 Tumor Virus Infections Virus Activation Virus Latency Activation of NF-κB via endosomal Toll-like receptor 7 (TLR7) or TLR9 suppresses murine herpesvirus 68 reactivation. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12891 2025-10-24T20:49:17Z

Knüpfer SC Liechti MD Mordasini L Abt D Engeler DS Wöllner J Pannek J Kiss B Burkhard FC Schneider MP Miramontes E Kessels AG Bachmann LM Kessler TM 2014 BACKGROUND Sacral neuromodulation has become a well-established and widely accepted treatment for refractory non-neurogenic lower urinary tract dysfunction, but its value in patients with a neurological cause is unclear. Although there is evidence indicating that sacral neuromodulation may be effective and safe for treating neurogenic lower urinary tract dysfunction, the number of investigated patients is low and there is a lack of randomized controlled trials. METHODS AND DESIGN This study is a prospective, randomized, placebo-controlled, double-blind multicenter trial including 4 sacral neuromodulation referral centers in Switzerland. Patients with refractory neurogenic lower urinary tract dysfunction are enrolled. After minimally invasive bilateral tined lead placement into the sacral foramina S3 and/or S4, patients undergo prolonged sacral neuromodulation testing for 3-6 weeks. In case of successful (defined as improvement of at least 50% in key bladder diary variables (i.e. number of voids and/or number of leakages, post void residual) compared to baseline values) prolonged sacral neuromodulation testing, the neuromodulator is implanted in the upper buttock. After a 2 months post-implantation phase when the neuromodulator is turned ON to optimize the effectiveness of neuromodulation using sub-sensory threshold stimulation, the patients are randomized in a 1:1 allocation in sacral neuromodulation ON or OFF. At the end of the 2 months double-blind sacral neuromodulation phase, the patients have a neuro-urological re-evaluation, unblinding takes place, and the neuromodulator is turned ON in all patients. The primary outcome measure is success of sacral neuromodulation, secondary outcome measures are adverse events, urodynamic parameters, questionnaires, and costs of sacral neuromodulation. DISCUSSION It is of utmost importance to know whether the minimally invasive and completely reversible sacral neuromodulation would be a valuable treatment option for patients with refractory neurogenic lower urinary tract dysfunction. If this type of treatment is effective in the neurological population, it would revolutionize the management of neurogenic lower urinary tract dysfunction. TRIAL REGISTRATION TRIAL REGISTRATION NUMBER http://www.clinicaltrials.gov; Identifier: NCT02165774. https://sonar.ch/global/documents/12891 eng info:eu-repo/semantics/altIdentifier/doi/10.1186/1471-2490-14-65 info:eu-repo/semantics/altIdentifier/pmid/25123172 BMC urology. - 2014 Analysis of Variance Double-Blind Method Electric Stimulation Therapy Electrodes, Implanted Humans Patient Selection Prospective Studies Research Design Sacrococcygeal Region Treatment Outcome Urinary Bladder, Neurogenic Protocol for a randomized, placebo-controlled, double-blind clinical trial investigating sacral neuromodulation for neurogenic lower urinary tract dysfunction. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12913 2025-10-24T20:49:18Z

Cantù C Zimmerli D Hausmann G Valenta T Moor A Aguet M Basler K 2014 Bcl9 and Bcl9l (Bcl9/9l) encode Wnt signaling components that mediate the interaction between β-catenin and Pygopus (Pygo) via two evolutionarily conserved domains, HD1 and HD2, respectively. We generated mouse strains lacking these domains to probe the β-catenin-dependent and β-catenin-independent roles of Bcl9/9l and Pygo during mouse development. While lens development is critically dependent on the presence of the HD1 domain, it is not affected by the lack of the HD2 domain, indicating that Bcl9/9l act in this context in a β-catenin-independent manner. Furthermore, we uncover a new regulatory circuit in which Pax6, the master regulator of eye development, directly activates Bcl9/9l transcription. application/pdf https://sonar.ch/global/documents/12913 eng info:eu-repo/semantics/altIdentifier/doi/10.1101/gad.246140.114 info:eu-repo/semantics/altIdentifier/pmid/25184676 info:eu-repo/semantics/openAccess Genes & development. - 2014 Bcl9 Pax6 Pygopus Wnt lens induction mouse Animals Cells, Cultured Eye Proteins Gene Expression Regulation, Developmental Gene Knock-In Techniques Homeodomain Proteins Intracellular Signaling Peptides and Proteins Lens, Crystalline Mice Mice, Inbred C57BL Neoplasm Proteins PAX6 Transcription Factor Paired Box Transcription Factors Protein Structure, Tertiary Repressor Proteins Signal Transduction Transcription Factors Wnt Proteins beta Catenin Pax6-dependent, but β-catenin-independent, function of Bcl9 proteins in mouse lens development. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12763 2025-10-24T20:49:20Z

Langer N Pedroni A Jäncke L 2013 Graph theory deterministically models networks as sets of vertices, which are linked by connections. Such mathematical representation of networks, called graphs are increasingly used in neuroscience to model functional brain networks. It was shown that many forms of structural and functional brain networks have small-world characteristics, thus, constitute networks of dense local and highly effective distal information processing. Motivated by a previous small-world connectivity analysis of resting EEG-data we explored implications of a commonly used analysis approach. This common course of analysis is to compare small-world characteristics between two groups using classical inferential statistics. This however, becomes problematic when using measures of inter-subject correlations, as it is the case in commonly used brain imaging methods such as structural and diffusion tensor imaging with the exception of fibre tracking. Since for each voxel, or region there is only one data point, a measure of connectivity can only be computed for a group. To empirically determine an adequate small-world network threshold and to generate the necessary distribution of measures for classical inferential statistics, samples are generated by thresholding the networks on the group level over a range of thresholds. We believe that there are mainly two problems with this approach. First, the number of thresholded networks is arbitrary. Second, the obtained thresholded networks are not independent samples. Both issues become problematic when using commonly applied parametric statistical tests. Here, we demonstrate potential consequences of the number of thresholds and non-independency of samples in two examples (using artificial data and EEG data). Consequently alternative approaches are presented, which overcome these methodological issues. application/pdf https://sonar.ch/global/documents/12763 eng info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0053199 info:eu-repo/semantics/altIdentifier/pmid/23301043 info:eu-repo/semantics/openAccess PloS one. - 2013 Adult Algorithms Brain Brain Mapping Data Interpretation, Statistical Electroencephalography Humans Intelligence Tests Male Models, Neurological Nerve Net Neural Pathways Young Adult The problem of thresholding in small-world network analysis. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12787 2025-10-24T20:49:32Z

van Eijk RP van der Zwan A Bleys RL Regli L Esposito G 2015 OBJECTIVE Postmortem CT angiography is a common procedure used to visualize the entire human vasculature. For visualization of a specific organ's vascular anatomy, casting is the preferred method. Because of the permanent and damaging nature of casting, the organ cannot be further used as an experimental model after angiography. Therefore, there is a need for a minimally traumatic method to visualize organ-specific vascular anatomy. The purpose of this study was to develop and evaluate a contrast enhancement technique that is capable of visualizing the intracranial vascular anatomy while preserving the anatomic integrity in cadaver heads. MATERIALS AND METHODS Seven human heads were used in this study. Heads were prepared by cannulating the vertebral and internal carotid arteries. Contrast agent was injected as a mixture of tap water, polyethylene glycol 600, and an iodinated contrast agent. Postmortem imaging was executed on a 64-MDCT scanner. Primary image review and 3D reconstruction were performed on a CT workstation. RESULTS Clear visualization of the major cerebral arteries and smaller intracranial branches was achieved. Adequate visualization was obtained for both the anterior and posterior intracranial circulation. The minimally traumatic angiography method preserved the vascular integrity of the cadaver heads. CONCLUSION A novel application of postmortem CT angiography is presented here. The technique can be used for radiologic evaluation of the intracranial circulation in cadaver heads. After CT angiography, the specimen can be used for further experimental or laboratory testing and teaching purposes. https://sonar.ch/global/documents/12787 eng info:eu-repo/semantics/altIdentifier/doi/10.2214/AJR.15.14500 info:eu-repo/semantics/altIdentifier/pmid/26587934 AJR. American journal of roentgenology. - 2015 CT angiography intracranial circulation postmortem Cadaver Cerebral Angiography Cerebrovascular Circulation Contrast Media Humans Imaging, Three-Dimensional Iohexol Radiographic Image Interpretation, Computer-Assisted Tomography, X-Ray Computed Novel Application of Postmortem CT Angiography for Evaluation of the Intracranial Vascular Anatomy in Cadaver Heads. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12793 2025-10-24T20:49:33Z

Ozdemir C Kucuksezer UC Akdis M Akdis CA 2016 Allergen immunotherapy (AIT) is an effective way to treat allergic disorders, targeting the underlying mechanisms and altering the disease course by inducing a long-lasting clinical and immune tolerance to allergens. Although sublingual and subcutaneous routes are used in daily practice, many novel ways to decrease side effects and duration and increase efficacy have been pursued. Further studies are needed to develop biomarkers for the identification of AIT responder patients and also to use the developed knowledge in allergy prevention studies. Future directions in AIT include treatments for autoimmune diseases, chronic infections, organ transplantation, and breaking immune tolerance to cancer cells. https://sonar.ch/global/documents/12793 eng info:eu-repo/semantics/altIdentifier/doi/10.1016/j.iac.2015.08.003 info:eu-repo/semantics/altIdentifier/pmid/26617228 Immunology and allergy clinics of North America. - 2016 Allergens Allergy Immunotherapy T regulatory cells Tolerance Administration, Sublingual Air Pollutants Allergens Animals Desensitization, Immunologic Environmental Exposure Humans Hypersensitivity Immune Tolerance Injections, Subcutaneous T-Lymphocytes, Regulatory Mechanisms of Aeroallergen Immunotherapy: Subcutaneous Immunotherapy and Sublingual Immunotherapy. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12808 2025-10-24T20:49:34Z

Bernasconi L Ramenzoni LL Al-Majid A Tini GM Graber SM Schmidlin PR Irani S 2015 OBJECTIVES To determine whether bronchial colonisations/infections with periodontopathogenic bacteria are associated with elevated inflammatory markers such as MMPs, interleukins and Tumor necrosis factor alpha in the bronchial fluid. METHODS Periodontal status was assessed in consecutive outpatients planned for elective bronchoscopies, and PCR for periodontopathogenic bacteria was performed from a protected specimen brush sample taken from the bronchial mucosa. Additionally, MMPs, interleukins and Tumor necrosis factor alpha were measured in the bronchial fluid. RESULTS Out of the four species assessed, one species was found in 13 of 91 (14%) patients, and two in 12 (13%), three in 13 (14%) and all four in 1 (1%) patient, respectively. In multiple linear regression models the presence of Treponema denticola showed a consistent pattern of positive effects in bronchial fluid (Bonferroni adjusted p-values) on the levels of MMP9 (p adj.: 0.028) and MMP12 (p adj.: 0.029). Active smoking was independently associated with increased levels of aMMP8 (p adj.: 0.005) and MMP9 (p adj.: 0.009). Levels of IL-1 ß, IL-8 and Tumor necrosis factor alpha measured in the bronchial fluid were not affected by the presence of periodontopathogenic bacteria. CONCLUSIONS Bronchial colonisation/infection with Treponema denticola and smoking are independently associated with elevated MMPs (MMP9/MMP12 and MMP8/MMP9, respectively) in the bronchial fluid. application/pdf https://sonar.ch/global/documents/12808 eng info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0144461 info:eu-repo/semantics/altIdentifier/pmid/26656474 info:eu-repo/semantics/openAccess PloS one. - 2015 Aged Bronchi Bronchoalveolar Lavage Fluid Bronchoscopy Cross-Sectional Studies Cytokines Female Humans Male Matrix Metalloproteinase 12 Matrix Metalloproteinase 8 Matrix Metalloproteinase 9 Middle Aged Periodontal Pocket Periodontitis Respiratory Mucosa Smoking Treponema denticola Elevated Matrix Metalloproteinase Levels in Bronchi Infected with Periodontopathogenic Bacteria. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12828 2025-10-24T20:49:36Z

Beyer T Lassen ML Boellaard R Delso G Yaqub M Sattler B Quick HH 2016 OBJECTIVE We assess inter- and intra-subject variability of magnetic resonance (MR)-based attenuation maps (MRμMaps) of human subjects for state-of-the-art positron emission tomography (PET)/MR imaging systems. MATERIALS AND METHODS Four healthy male subjects underwent repeated MR imaging with a Siemens Biograph mMR, Philips Ingenuity TF and GE SIGNA PET/MR system using product-specific MR sequences and image processing algorithms for generating MRμMaps. Total lung volumes and mean attenuation values in nine thoracic reference regions were calculated. Linear regression was used for comparing lung volumes on MRμMaps. Intra- and inter-system variability was investigated using a mixed effects model. RESULTS Intra-system variability was seen for the lung volume of some subjects, (p = 0.29). Mean attenuation values across subjects were significantly different (p < 0.001) due to different segmentations of the trachea. Differences in the attenuation values caused noticeable intra-individual and inter-system differences that translated into a subsequent bias of the corrected PET activity values, as verified by independent simulations. CONCLUSION Significant differences of MRμMaps generated for the same subjects but different PET/MR systems resulted in differences in attenuation correction factors, particularly in the thorax. These differences currently limit the quantitative use of PET/MR in multi-center imaging studies. https://sonar.ch/global/documents/12828 eng info:eu-repo/semantics/altIdentifier/doi/10.1007/s10334-015-0505-4 info:eu-repo/semantics/altIdentifier/pmid/26739263 Magma (New York, N.Y.). - 2016 Accuracy Combined PET/MR MR-based attenuation correction Reproducibility Whole-body hybrid imaging Adult Algorithms Computer Simulation Humans Image Processing, Computer-Assisted Lung Magnetic Resonance Imaging Male Middle Aged Multimodal Imaging Positron-Emission Tomography Reproducibility of Results Whole Body Imaging Investigating the state-of-the-art in whole-body MR-based attenuation correction: an intra-individual, inter-system, inventory study on three clinical PET/MR systems. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12836 2025-10-24T20:49:36Z

Wettstein MS Poyet C Grossmann NC Fankhauser CD Keller EX Kozomara M Meyer S Sulser T Müller A Hermanns T 2016 PURPOSE To assess whether and to what extent irrigation fluid absorption occurs during laser vaporization (LV) of the prostate using the 180 W XPS™ GreenLight laser. METHODS This prospective investigation was performed in a tertiary care center with a consecutive series of patients undergoing 180 W LV of the prostate. Intraoperative irrigation was performed with isotonic saline containing 1 % ethanol. The volume of irrigation fluid absorption was calculated from periodically performed breath ethanol measurements during LV. Additionally, intraoperative changes in biochemical and hematological blood parameters were assessed. RESULTS Positive breath ethanol tests were detectable in 22 of 54 patients. The median absorption volume in these patients was 950 ml (range 208-4579 ml). Ten patients absorbed more than 2000 ml. Absorbers had smaller prostates, more capsular perforations and injuries to venous sinuses, and more total energy was applied with higher output power. Five patients had transient symptoms potentially related to fluid absorption. A significant drop in hemoglobin, hematocrit, venous pH and bicarbonate and an increase in chloride were detectable in the absorber group. These changes were significantly different in the non-absorber group. CONCLUSIONS Absorption of irrigation fluid did occur in a relevant proportion of patients undergoing XPS™ GreenLight LV. High-volume absorption (≥2000 ml), which might be clinically relevant, was detectable in almost 20 % of all procedures. Absorption of saline irrigation fluid does not result in a classical TUR syndrome, but fluid and chloride overload can lead to serious complications, particularly in cardiovascular high-risk patients. Thus, patients with symptoms potentially related to fluid absorption should be monitored carefully. application/pdf https://sonar.ch/global/documents/12836 eng info:eu-repo/semantics/altIdentifier/doi/10.1007/s00345-016-1766-x info:eu-repo/semantics/altIdentifier/pmid/26780733 info:eu-repo/semantics/openAccess World journal of urology. - 2016 Ethanol Intraoperative complications Laser therapy Prostate Therapeutic irrigation Absorption, Physiological Aged Aged, 80 and over Breath Tests Ethanol Humans Intraoperative Complications Laser Therapy Male Middle Aged Prospective Studies Prostatectomy Sodium Chloride Therapeutic Irrigation Absorption of irrigation fluid during XPS™ GreenLight laser vaporization of the prostate: results from a prospective breath ethanol monitoring study. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12882 2025-10-24T20:49:40Z

Meo M Meste O Signore S Sorrentino A Cannata A Zhou Y Matsuda A Luciani M Kannappan R Goichberg P Leri A Anversa P Rota M 2016 BACKGROUND Diabetes is associated with prolongation of the QT interval of the electrocardiogram and enhanced dispersion of ventricular repolarization, factors that, together with atherosclerosis and myocardial ischemia, may promote the occurrence of electrical disorders. Thus, we tested the possibility that alterations in transmembrane ionic currents reduce the repolarization reserve of myocytes, leading to action potential (AP) prolongation and enhanced beat-to-beat variability of repolarization. METHODS AND RESULTS Diabetes was induced in mice with streptozotocin (STZ), and effects of hyperglycemia on electrical properties of whole heart and myocytes were studied with respect to an untreated control group (Ctrl) using electrocardiographic recordings in vivo, ex vivo perfused hearts, and single-cell patch-clamp analysis. Additionally, a newly developed algorithm was introduced to obtain detailed information of the impact of high glucose on AP profile. Compared to Ctrl, hyperglycemia in STZ-treated animals was coupled with prolongation of the QT interval, enhanced temporal dispersion of electrical recovery, and susceptibility to ventricular arrhythmias, defects observed, in part, in the Akita mutant mouse model of type I diabetes. AP was prolonged and beat-to-beat variability of repolarization was enhanced in diabetic myocytes, with respect to Ctrl cells. Density of Kv K(+) and L-type Ca(2+) currents were decreased in STZ myocytes, in comparison to cells from normoglycemic mice. Pharmacological reduction of Kv currents in Ctrl cells lengthened AP duration and increased temporal dispersion of repolarization, reiterating features identified in diabetic myocytes. CONCLUSIONS Reductions in the repolarizing K(+) currents may contribute to electrical disturbances of the diabetic heart. application/pdf https://sonar.ch/global/documents/12882 eng info:eu-repo/semantics/altIdentifier/doi/10.1161/JAHA.115.003078 info:eu-repo/semantics/altIdentifier/pmid/26896476 info:eu-repo/semantics/openAccess Journal of the American Heart Association. - 2016 diabetes electrophysiology repolarization variability Action Potentials Algorithms Animals Arrhythmias, Cardiac Blood Glucose Diabetes Mellitus, Experimental Diabetic Cardiomyopathies Electrocardiography Female Heart Rate Kinetics Male Mice, Inbred C57BL Myocytes, Cardiac Patch-Clamp Techniques Potassium Potassium Channels Signal Processing, Computer-Assisted Reduction in Kv Current Enhances the Temporal Dispersion of the Action Potential in Diabetic Myocytes: Insights From a Novel Repolarization Algorithm. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12914 2025-10-24T20:49:42Z

Ziv C Malitsky S Othman A Ben-Dor S Wei Y Zheng S Aharoni A Hornemann T Vardi A 2016 Marine viruses are the most abundant biological entities in the oceans shaping community structure and nutrient cycling. The interaction between the bloom-forming alga Emiliania huxleyi and its specific large dsDNA virus (EhV) is a major factor determining the fate of carbon in the ocean, thus serving as a key host-pathogen model system. The EhV genome encodes for a set of genes involved in the de novo sphingolipid biosynthesis, not reported in any viral genome to date. We combined detailed lipidomic and biochemical analyses to characterize the functional role of this virus-encoded pathway during lytic viral infection. We identified a major metabolic shift, mediated by differential substrate specificity of virus-encoded serine palmitoyltransferase, a key enzyme of sphingolipid biosynthesis. Consequently, unique viral glycosphingolipids, composed of unusual hydroxylated C17 sphingoid bases (t17:0) were highly enriched in the infected cells, and their synthesis was found to be essential for viral assembly. These findings uncover the biochemical bases of the virus-induced metabolic rewiring of the host sphingolipid biosynthesis during the chemical "arms race" in the ocean. application/pdf https://sonar.ch/global/documents/12914 eng info:eu-repo/semantics/altIdentifier/doi/10.1073/pnas.1523168113 info:eu-repo/semantics/altIdentifier/pmid/26984500 info:eu-repo/semantics/openAccess Proceedings of the National Academy of Sciences of the United States of America. - 2016 Emiliania huxelyi algal blooms marine viruses serine palmitoyltransferase sphingolipids DNA Viruses Eutrophication Gene Expression Regulation, Viral Glycosphingolipids Haptophyta Host-Pathogen Interactions Hydroxylation Plant Diseases Serine C-Palmitoyltransferase Sphingolipids Viral Proteins Viral serine palmitoyltransferase induces metabolic switch in sphingolipid biosynthesis and is required for infection of a marine alga. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12919 2025-10-24T20:49:42Z

Jentzsch T Rahm S Seifert B Farei-Campagna J Werner CM Bouaicha S 2016 PURPOSE To investigate the association between arthroscopy simulator performance and video game skills. METHODS This study compared the performances of 30 volunteers without experience performing arthroscopies in 3 different tasks of a validated virtual reality knee arthroscopy simulator with the video game experience using a questionnaire and actual performances in 5 different 2- and 3-dimensional (D) video games of varying genres on 2 different platforms. RESULTS Positive correlations between knee arthroscopy simulator and video game performances (ρ = 0.63, P < .001) as well as experiences (ρ = 0.50, P = .005) were found. The strongest correlations were found for the task of catching (hooking) 6 foreign bodies (virtual rings; "triangulation") and the dribbling performance in a sports game and a first-person shooter game, as well as the meniscus resection and a tile-matching puzzle game (all ρ ≥ 0.60, P < .001). No correlations were found for any of the knee arthroscopy simulator tasks and a strategy game. CONCLUSIONS Although knee arthroscopy performances do not correlate with 2-D strategy video game skills, they show a correlation with 2-D tile-matching puzzle games only for easier tasks with a rather limited focus, and highly correlate with 3-D sports and first-person shooter video games. These findings show that experienced and good 3-D gamers are better arthroscopists than nonexperienced and poor 3-D gamers. LEVEL OF EVIDENCE Level II, observational cross-sectional study. https://sonar.ch/global/documents/12919 eng info:eu-repo/semantics/altIdentifier/doi/10.1016/j.arthro.2015.12.047 info:eu-repo/semantics/altIdentifier/pmid/27006104 Arthroscopy : the journal of arthroscopic & related surgery : official publication of the Arthroscopy Association of North America and the International Arthroscopy Association. - 2016 Adult Arthroscopy Computer Simulation Cross-Sectional Studies Female Humans Knee Joint Male Simulation Training Video Games Correlation Between Arthroscopy Simulator and Video Game Performance: A Cross-Sectional Study of 30 Volunteers Comparing 2- and 3-Dimensional Video Games. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12803 2025-10-24T20:49:43Z

O'Neill F Riwanto M Charakida M Colin S Manz J McLoughlin E Khan T Klein N Kay CW Patel K Chinetti G Staels B D'Aiuto F Landmesser U Deanfield J 2015 OBJECTIVE HDL functionality has been shown to be impaired in inflammatory conditions, including coronary artery disease. The present study aims to determine the impact of low grade and acute inflammation on HDL function and structure. APPROACH AND RESULTS i) The endothelial protective effects of HDL were compared between 26 periodontal patients and 26 age and sex matched controls by measuring paraoxonase activity in serum and nitric oxide bioavailability and superoxide production in endothelial cells. Paraoxonase activity and nitric oxide bioavailability were reduced, while superoxide production was increased (p<0.01) in periodontal patients compared to controls. ii) HDL function, including cholesterol efflux and vascular cell adhesion molecule-1 expression, was subsequently measured in the periodontal patients following an inflammatory stimulus. There was an acute deterioration in HDL's endothelial protective function, without change in cholesterol efflux, after 24h (p<0.01 for all). These functional changes tracked increases of inflammatory markers and altered HDL composition. Finally, HDL function returned to baseline levels after resolution of inflammation. CONCLUSION This study demonstrates that even minor alterations in systemic inflammation can impair the endothelial protective effects of HDL. These functional changes were independent of cholesterol efflux and were associated with remodeling of the HDL proteome. All measures of HDL's endothelial protective functions recovered with resolution of inflammation. These findings suggest that HDL dysfunction may represent a novel mechanism linking inflammation with progression of atheroma. https://sonar.ch/global/documents/12803 eng info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijcard.2015.03.058 info:eu-repo/semantics/altIdentifier/pmid/25919891 International journal of cardiology. - 2015 Atherosclerosis High-density lipoprotein Inflammation Adult Aryldialkylphosphatase Case-Control Studies Chronic Disease Endothelial Cells Female Follow-Up Studies Humans Inflammation Inflammation Mediators Lipoproteins, HDL Longitudinal Studies Male Middle Aged Nitric Oxide Periodontitis Severity of Illness Index Time Factors Vascular Cell Adhesion Molecule-1 Structural and functional changes in HDL with low grade and chronic inflammation. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12817 2025-10-24T20:49:45Z

Starck CT Steffel J Caliskan E Holubec T Schoenrath F Maisano F Falk V 2016 AIMS We investigated the safety and efficacy of a new bidirectional rotational mechanical extraction sheath (Evolution RL, Cook Medical, USA). METHODS AND RESULTS From April 2013 until September 2014, we performed lead extraction procedures in 71 patients with 112 leads scheduled for extraction. During this time period, we used the new Evolution RL rotational sheath in 40 patients on 52 leads (24 pacemaker leads and 28 implantable cardioverter-defibrillator leads) scheduled for extraction. The mean lead implant duration in these patients was 100.2 (22-271) months. Forty leads were right ventricular leads, 10 right atrial leads, and 2 coronary sinus leads. Clinical success among the leads approached with the Evolution RL device was 98.1%. Operative mortality was zero. Major complications did not occur. Four minor complications were encountered (all pocket haematomas). No device-related complications were noted. In cases with multiple leads in situ, no wrapping of companion leads was seen. The average number of extraction tools used per lead was 2.4 (2-4) in the Evolution RL cohort. CONCLUSION Based on the presented results of our initial experience with the new bidirectional rotational mechanical extraction device, its use is safe and efficient with high success rates in long implanted leads. https://sonar.ch/global/documents/12817 eng info:eu-repo/semantics/altIdentifier/doi/10.1093/europace/euv126 info:eu-repo/semantics/altIdentifier/pmid/25995394 Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology. - 2016 ICD Lead extraction Pacemaker Adult Aged Aged, 80 and over Cardiac Catheterization Cardiac Catheters Defibrillators, Implantable Device Removal Female Humans Male Middle Aged Pacemaker, Artificial Prosthesis Design Retrospective Studies Treatment Outcome Clinical performance of a new bidirectional rotational mechanical lead extraction sheath. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12820 2025-10-24T20:49:45Z

Schörg A Santambrogio S Platt JL Schödel J Lindenmeyer MT Cohen CD Schrödter K Mole DR Wenger RH Hoogewijs D 2015 A crucial step in the cellular adaptation to oxygen deficiency is the binding of hypoxia-inducible factors (HIFs) to hypoxia response elements (HREs) of oxygen-regulated genes. Genome-wide HIF-1α/2α/β DNA-binding studies revealed that the majority of HREs reside distant to the promoter regions, but the function of these distal HREs has only been marginally studied in the genomic context. We used chromatin immunoprecipitation (ChIP), gene editing (TALEN) and chromosome conformation capture (3C) to localize and functionally characterize a 82 kb upstream HRE that solely drives oxygen-regulated expression of the newly identified HIF target gene PAG1. PAG1, a transmembrane adaptor protein involved in Src signalling, was hypoxically induced in various cell lines and mouse tissues. ChIP and reporter gene assays demonstrated that the -82 kb HRE regulates PAG1, but not an equally distant gene further upstream, by direct interaction with HIF. Ablation of the consensus HRE motif abolished the hypoxic induction of PAG1 but not general oxygen signalling. 3C assays revealed that the -82 kb HRE physically associates with the PAG1 promoter region, independent of HIF-DNA interaction. These results demonstrate a constitutive interaction between the -82 kb HRE and the PAG1 promoter, suggesting a physiologically important rapid response to hypoxia. application/pdf https://sonar.ch/global/documents/12820 eng info:eu-repo/semantics/altIdentifier/doi/10.1093/nar/gkv506 info:eu-repo/semantics/altIdentifier/pmid/26007655 info:eu-repo/semantics/openAccess Nucleic acids research. - 2015 Adaptor Proteins, Signal Transducing Animals Basic Helix-Loop-Helix Transcription Factors Cell Hypoxia Cell Line Chromatin HeLa Cells Humans Hypoxia-Inducible Factor 1 Membrane Proteins Mice Mice, Inbred C57BL Phosphoproteins Promoter Regions, Genetic Response Elements Signal Transduction Transcriptional Activation src-Family Kinases Destruction of a distal hypoxia response element abolishes trans-activation of the PAG1 gene mediated by HIF-independent chromatin looping. http://purl.org/coar/resource_type/c_6501

oai:sonar.ch:12842 2025-10-24T20:49:47Z

Paanalahti K Wertli MM Held U Åkerstedt T Holm LW Nordin M Skillgate E 2016 PURPOSE The estimated prevalence of poor sleep in patients with non-specific chronic low back pain is estimated to 64% in the adult population. The annual cost for musculoskeletal pain and reported poor sleep is estimated to be billions of dollars annually in the US. The aim of this cohort study with one-year follow-up was to explore the role of impaired sleep with daytime consequence on the prognosis of non-specific neck and/or back pain. METHODS Secondary analysis of a randomized controlled trial, including 409 patients. RESULTS Patients with good sleep at baseline were more likely to experience a minimal clinically important difference in pain [OR 2.03 (95% CI 1.22-3.38)] and disability [OR 1.85 (95% CI 1.04-3.30)] compared to patients with impaired sleep at one-year follow-up. CONCLUSION Patients with non-specific neck and/or back pain and self-reported good sleep are more likely to experience a minimal clinically important difference in pain and disability compared to patients with impaired sleep with daytime consequence. https://sonar.ch/global/documents/12842 eng info:eu-repo/semantics/altIdentifier/doi/10.1007/s00586-015-3987-x info:eu-repo/semantics/altIdentifier/pmid/26063054 European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society. - 2016 Back pain Impaired sleep Naprapathy Neck pain Spinal pain Adult Back Pain Cohort Studies Disability Evaluation Female Humans Longitudinal Studies Male Middle Aged Musculoskeletal Manipulations Neck Pain Pain Measurement Prognosis Sleep Wake Disorders Treatment Outcome Spinal pain--good sleep matters: a secondary analysis of a randomized controlled trial. http://purl.org/coar/resource_type/c_6501 .eJyN0EFzojAYxvHvkrOzkwBOxZke1tWA7CpNwCTk0omEAksAFVwpnX73Otueq-_9_c0z_zfQZZkGc_hjimzHnboP0LZnFkQPE3BQeQbm1gRUF3XKOzB_A3XWK6169XTKXsoBzEGrymedgvcJ6NJTa8xzecUA9sw5sQYkPfaSWO5Ze8zR2EWam2rZYCRFMGVeYfaVNNrbtr8WCSQjDoSNRchInzD3-EeYKl7mKNzJ5uf_e62FDXmEF2P6d-PQ6hCHfIUInE3p6tBd_896kL8zw5ytF3QSU2dr2FEzM-68voyXkn46MG8cwimTRSiKOlqxXVzjZGPpKBHEyvw1vDplDAuf8sslNlRszRpJKBtSo0rihf21B6dPa87q4F805q977FLl41HyXsk6OMZ-ahF6r0NuOPf2cW71GXgTtJKliLAAa37AKWSR8lm7h8VXn7HcmBlPBenlii1SqCPNmaBxIMMlRVk9BPJux7nl3Nln830f8vgI3j8AF1fq_A.aumPGg.3MSG93aYZEtQC2mGGN-QCFWy4Yc