Lymphatic endothelial cells prime naïve CD8+ T cells into memory cells under steady-state conditions.
- Vokali E Institute of Bioengineering, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
- Yu SS Institute of Bioengineering, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
- Hirosue S Institute of Bioengineering, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
- Rinçon-Restrepo M Institute of Bioengineering, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
- V Duraes F Department of Pathology and Immunology, Faculty of Medicine, University of Geneva, Geneva, Switzerland.
- Scherer S Swiss Vaccine Research Institute, Epalinges, Switzerland.
- Corthésy-Henrioud P Institute of Bioengineering, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
- Kilarski WW Institute of Bioengineering, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
- Mondino A Division of Immunology, Transplantation and Infectious Diseases, San Raffaele Scientific Institute, Milan, Italy.
- Zehn D Swiss Vaccine Research Institute, Epalinges, Switzerland.
- Hugues S Department of Pathology and Immunology, Faculty of Medicine, University of Geneva, Geneva, Switzerland.
- Swartz MA Institute of Bioengineering, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland. melodyswartz@uchicago.edu.
- 2020-01-29
Published in:
- Nature communications. - 2020
Animals
CD8-Positive T-Lymphocytes
Cell Proliferation
Endothelial Cells
Gene Expression Profiling
Immunologic Memory
Mice, Inbred C57BL
Mice, Transgenic
English
Lymphatic endothelial cells (LECs) chemoattract naïve T cells and promote their survival in the lymph nodes, and can cross-present antigens to naïve CD8+ T cells to drive their proliferation despite lacking key costimulatory molecules. However, the functional consequence of LEC priming of CD8+ T cells is unknown. Here, we show that while many proliferating LEC-educated T cells enter early apoptosis, the remainders comprise a long-lived memory subset, with transcriptional, metabolic, and phenotypic features of central memory and stem cell-like memory T cells. In vivo, these memory cells preferentially home to lymph nodes and display rapid proliferation and effector differentiation following memory recall, and can protect mice against a subsequent bacterial infection. These findings introduce a new immunomodulatory role for LECs in directly generating a memory-like subset of quiescent yet antigen-experienced CD8+ T cells that are long-lived and can rapidly differentiate into effector cells upon inflammatory antigenic challenge.
- Language
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- English
- Open access status
- gold
- Identifiers
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- DOI 10.1038/s41467-019-14127-9
- PMID 31988323
- Persistent URL
- https://sonar.ch/global/documents/100408
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