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Designed ankyrin repeat proteins as anti-idiotypic-binding molecules.
Journal article

Designed ankyrin repeat proteins as anti-idiotypic-binding molecules.

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  • 2007-09-06
Published in:
  • Annals of the New York Academy of Sciences. - 2007
Animals
Ankyrin Repeat
Antibodies, Anti-Idiotypic
Cell Line
Humans
Mice
Protein Engineering
Rats
Receptors, IgE
English According to the network theory antibodies may act as antigens thus generating anti-idiotypic antibodies that can function as regulators of immune responses. Designed ankyrin repeat proteins (DARPins) are a new class of binding proteins and may serve as an alternative to antibodies. Selections from large DARPin libraries against the variable regions of a murine monoclonal anti-human IgE antibody, termed BSW17, yielded two highly specific anti-idiotypic DARPins both with high affinity. Their binding characteristics were comparable with these of a previously selected anti-idiotypic antibody. In vitro cell assays showed that the anti-idiotypic DARPins were able to inhibit the binding of BSW17 to cell-bound IgE and prevented BSW17 functional activity. These experiments demonstrate the possibility to isolate anti-idiotypic DARPins recognizing idiotypic determinants analogous to antibodies. In the future these DARPins may be further analyzed for their potential as putative vaccine candidates.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/101562
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