Journal article
Biofilm formation on restorative materials and resin composite cements.
- Astasov-Frauenhoffer M Department of Preventive Dentistry and Oral Microbiology, University Center for Dental Medicine, University of Basel, Hebelstrasse 3, 4056 Basel, Switzerland.
- Glauser S Division of Dental Materials and Engineering, Department of Reconstructive Dentistry, University Center for Dental Medicine, University of Basel, Hebelstrasse 3, 4056 Basel, Switzerland.
- Fischer J Division of Dental Materials and Engineering, Department of Reconstructive Dentistry, University Center for Dental Medicine, University of Basel, Hebelstrasse 3, 4056 Basel, Switzerland.
- Schmidli F Division of Dental Materials and Engineering, Department of Reconstructive Dentistry, University Center for Dental Medicine, University of Basel, Hebelstrasse 3, 4056 Basel, Switzerland.
- Waltimo T Department of Preventive Dentistry and Oral Microbiology, University Center for Dental Medicine, University of Basel, Hebelstrasse 3, 4056 Basel, Switzerland.
- Rohr N Division of Dental Materials and Engineering, Department of Reconstructive Dentistry, University Center for Dental Medicine, University of Basel, Hebelstrasse 3, 4056 Basel, Switzerland. Electronic address: nadja.rohr@unibas.ch.
- 2018-09-18
Published in:
- Dental materials : official publication of the Academy of Dental Materials. - 2018
Biofilm
Prosthetic treatment
Resin composite cement
Restorative material
Surface free energy
Wettability
Zirconia implant
Biofilms
Ceramics
Composite Resins
Dental Materials
Fusobacterium nucleatum
Materials Testing
Microscopy, Electron, Scanning
Porphyromonas gingivalis
Resin Cements
Spectrometry, X-Ray Emission
Streptococcus sanguis
Surface Properties
Wettability
Zirconium
English
OBJECTIVES
Monolithic zirconia, polymer-infiltrated ceramic and acrylate polymer cemented with resin composite cement have recently been identified as prosthetic treatment options for zirconia implants. The aim of the present study is to determine in vitro, to what extent bacteria adhere to these materials.
METHODS
Disks made of zirconia (Vita YZ [YZ]), polymer-infiltrated ceramic (Vita Enamic [VE]), acrylate polymer (Vita CAD-Temp [CT]), self-adhesive cement (RelyX Unicem 2 Automix [RUN]) and of two different adhesive cements (RelyX Ulimate [RUL] and Vita Adiva F-Cem [VAF]) were produced. The biofilm formation of three bacterial species (Streptococcus sanguinis, Fusobacterium nucleatum, Porphyromonas gingivalis) on each material was assessed over 72h using a flow chamber system. The biofilms were quantified by crystal violet staining (optical density 595nm) and visualized using SEM. The inorganic composition of the different materials was analyzed and the wettability of the specimens was measured.
RESULTS
For the restorative materials lowest biofilm formation was found on CT: OD 0.5±0.1, followed by VE: OD 0.8±0.1 and YZ: OD 1.4±0.3. The biofilm formation on resin composite cements was significantly lower on VAF: OD 0.6±0.1 than for RUL: OD 0.9±0.1 and RUN: OD 1.0±0.1. A high wettability of the specimens with saliva/serum mixture tended to result in a higher biofilm formation. Correlations were obtained between the organic/inorganic composition of the materials and the polar/dispersive part of the surface free energy.
SIGNIFICANCE
Three-species biofilm formation on restorative and cement materials strongly relies on the materials composition. If the restorative material CT and cement VAF also prevent excessive biofilm formation in a clinical situation should be further investigated.
Monolithic zirconia, polymer-infiltrated ceramic and acrylate polymer cemented with resin composite cement have recently been identified as prosthetic treatment options for zirconia implants. The aim of the present study is to determine in vitro, to what extent bacteria adhere to these materials.
METHODS
Disks made of zirconia (Vita YZ [YZ]), polymer-infiltrated ceramic (Vita Enamic [VE]), acrylate polymer (Vita CAD-Temp [CT]), self-adhesive cement (RelyX Unicem 2 Automix [RUN]) and of two different adhesive cements (RelyX Ulimate [RUL] and Vita Adiva F-Cem [VAF]) were produced. The biofilm formation of three bacterial species (Streptococcus sanguinis, Fusobacterium nucleatum, Porphyromonas gingivalis) on each material was assessed over 72h using a flow chamber system. The biofilms were quantified by crystal violet staining (optical density 595nm) and visualized using SEM. The inorganic composition of the different materials was analyzed and the wettability of the specimens was measured.
RESULTS
For the restorative materials lowest biofilm formation was found on CT: OD 0.5±0.1, followed by VE: OD 0.8±0.1 and YZ: OD 1.4±0.3. The biofilm formation on resin composite cements was significantly lower on VAF: OD 0.6±0.1 than for RUL: OD 0.9±0.1 and RUN: OD 1.0±0.1. A high wettability of the specimens with saliva/serum mixture tended to result in a higher biofilm formation. Correlations were obtained between the organic/inorganic composition of the materials and the polar/dispersive part of the surface free energy.
SIGNIFICANCE
Three-species biofilm formation on restorative and cement materials strongly relies on the materials composition. If the restorative material CT and cement VAF also prevent excessive biofilm formation in a clinical situation should be further investigated.
- Language
-
- English
- Open access status
- closed
- Identifiers
-
- DOI 10.1016/j.dental.2018.08.300
- PMID 30220506
- Persistent URL
- https://sonar.ch/global/documents/102236
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