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Genotoxicity, acute and subchronic toxicity evaluation of savory food ingredients.
Journal article

Genotoxicity, acute and subchronic toxicity evaluation of savory food ingredients.

  • Tafazoli S Intertek Scientific and Regulatory Consultancy, 2233 Argentia Road, Suite 201, Mississauga L5N 2X7, ON, Canada.
  • Vo TD Intertek Scientific and Regulatory Consultancy, 2233 Argentia Road, Suite 201, Mississauga L5N 2X7, ON, Canada.
  • Petersen A Nestlé Product Technology Centre Lebensmittelforschung GmbH, Lange Str.21, 78224 Singen, Germany.
  • Constable A Nestec Ltd., P.O. Box 44, CH-1000 Lausanne 26, Switzerland.
  • Coulet M Nestec Ltd., P.O. Box 44, CH-1000 Lausanne 26, Switzerland. Electronic address: Myriam.Coulet@rdls.nestle.com.
  • Phothirath P Nestlé Institute of Health Sciences SA, EPFL Innovation Park, Building H, CH-1015 Lausanne, Switzerland.
  • Lang J Aurigon GmbH, Machtlfinger Straße 13, 81379 München, Germany.
  • Baldwin N Intertek Scientific & Regulatory Consultancy, Room 1036, Building A8, Cody Technology Park, Ively Road, Farnborough, Hampshire, GU14 0LX, UK.
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  • 2017-05-11
Published in:
  • Regulatory toxicology and pharmacology : RTP. - 2017
Formic acid
Genotoxicity
Glutamic acid
Inosine 5′-monophosphate
L-alanine
Rat
Safety
Subchronic toxicity
Succinic acid
glycine
Animals
DNA Damage
Female
Fermentation
Food Ingredients
Heart
Kidney
Liver
Male
Mutagenicity Tests
Mutagens
No-Observed-Adverse-Effect Level
Rats
Satureja
Toxicity Tests, Acute
Toxicity Tests, Subchronic
English The potential toxicity of two savory food ingredients produced by fermentation of enzymatically hydrolyzed corn starch (Savory Base 100 and Savory Base 200) was evaluated individually in a bacterial reverse mutation assay, an in vitro mammalian cell gene mutation assay, an acute oral study and as a mixture in a 90-day dietary study. In the bacterial reverse mutation and in vitro mammalian cell gene mutation assays, neither ingredient was mutagenic at concentrations up to 5000 μg/plate and 5000 μg/mL, respectively in the presence and absence of metabolic activation. In the acute study, the no-observed-adverse-effect level (NOAEL) for each Savory Base 100 and Savory Base 200 in male and female rats was 2000 mg/kg body weight. In the 90-day study, the hematology and clinical chemistry findings and histopathological changes noted in the liver, heart and kidneys were deemed to be of no toxicological significance, as the mean values were within the historical control range, were not dose-dependent, occurred at a similar frequency in control groups, or only occurred in the control group. Considering these findings, the NOAEL for Savory Base 100 and Savory Base 200 was 2333 and 1167 mg/kg body weight, respectively, the highest dose tested in each case.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/102562
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