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Novel strategies for expansion of tooth epithelial stem cells and ameloblast generation.

  • Binder M Research Program in Developmental Biology, Institute of Biotechnology, University of Helsinki, Helsinki, Finland.
  • Biggs LC Helsinki Institute of Life Science, Biomedicum Helsinki, University of Helsinki, Helsinki, Finland.
  • Kronenberg MS Center for Regenerative Medicine and Skeletal Development, School of Dental Medicine, UConn Health, Farmington, CT, USA.
  • Schneider P Department of Biochemistry, University of Lausanne, CH-1066, Epalinges, Switzerland.
  • Thesleff I Research Program in Developmental Biology, Institute of Biotechnology, University of Helsinki, Helsinki, Finland.
  • Balic A Research Program in Developmental Biology, Institute of Biotechnology, University of Helsinki, Helsinki, Finland. anamaria.balic@helsinki.fi.
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  • 2020-03-20
Published in:
  • Scientific reports. - 2020
Ameloblasts
Animals
Cell Differentiation
Cells, Cultured
Coculture Techniques
Epithelial Cells
Mice
Mice, Inbred C57BL
Signal Transduction
Stem Cells
Tooth
English Enamel is secreted by ameloblasts derived from tooth epithelial stem cells (SCs). Humans cannot repair or regenerate enamel, due to early loss of tooth epithelial SCs. Contrarily in the mouse incisors, epithelial SCs are maintained throughout life and endlessly generate ameloblasts, and thus enamel. Here we isolated Sox2-GFP+ tooth epithelial SCs which generated highly cellular spheres following a novel in vitro strategy. This system enabled analysis of SC regulation by various signaling molecules, and supported the stimulatory and inhibitory roles of Shh and Bmp, respectively; providing better insight into the heterogeneity of the SCs. Further, we generated a novel mouse reporter, Enamelin-tdTomato for identification of ameloblasts in live tissues and cells, and used it to demonstrate presence of ameloblasts in the new 3D co-culture system of dental SCs. Collectively, our results provide means of generating 3D tooth epithelium from adult SCs which can be utilized toward future generation of enamel.
Language
  • English
Open access status
gold
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Persistent URL
https://sonar.ch/global/documents/1029
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