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Canonical wnt signaling is required for commissural axon guidance.
Journal article

Canonical wnt signaling is required for commissural axon guidance.

  • Avilés EC Institute of Molecular Life Sciences and Neuroscience Center Zurich, University of Zurich, Winterthurerstrasse 190, 8057, Zurich, Switzerland.
  • Stoeckli ET Institute of Molecular Life Sciences and Neuroscience Center Zurich, University of Zurich, Winterthurerstrasse 190, 8057, Zurich, Switzerland.
  • 2015-05-28
Published in:
  • Developmental neurobiology. - 2016
Lrp5
Lrp6
PCP pathway
chicken embryo
dI1 neurons
in ovo RNAi
Animals
Axons
Chick Embryo
Chickens
Chlorocebus aethiops
Gene Expression Regulation, Developmental
Gene Silencing
Neurogenesis
Spinal Cord
Wnt Proteins
Wnt Signaling Pathway
English Morphogens have been identified as guidance cues for postcrossing commissural axons in the spinal cord. Shh has a dual effect on postcrossing commissural axons: a direct repellent effect mediated by Hhip as a receptor, and an indirect effect by shaping a Wnt activity gradient. Wnts were shown to be attractants for postcrossing commissural axons in both chicken and mouse embryos. In mouse, the effects of Wnts on axon guidance were concluded to depend on the planar cell polarity (PCP) pathway. Canonical Wnt signaling was excluded based on the absence of axon guidance defects in mice lacking Lrp6 which is an obligatory coreceptor for Fzd in canonical Wnt signaling. In the loss-of-function studies reported here, we confirmed a role for the PCP pathway in postcrossing commissural axon guidance also in the chicken embryo. However, taking advantage of the precise temporal control of gene silencing provided by in ovo RNAi, we demonstrate that canonical Wnt signaling is also required for proper guidance of postcrossing commissural axons in the developing spinal cord. Thus, axon guidance does not seem to depend on any one of the classical Wnt signaling pathways but rather involve a network of Wnt receptors and downstream components.
Language
  • English
Open access status
hybrid
Identifiers
Persistent URL
https://sonar.ch/global/documents/102965
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