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Visualization of ER-to-Golgi transport in living cells reveals a sequential mode of action for COPII and COPI.
Journal article

Visualization of ER-to-Golgi transport in living cells reveals a sequential mode of action for COPII and COPI.

  • 1997-10-10
Published in:
  • Cell. - 1997
Animals
Biological Transport
Carrier Proteins
Cell Compartmentation
Cell Membrane
Chlorocebus aethiops
Coatomer Protein
Endoplasmic Reticulum
Exocytosis
Golgi Apparatus
Green Fluorescent Proteins
Luminescent Proteins
Membrane Glycoproteins
Membrane Proteins
Microtubules
Phosphoproteins
Recombinant Fusion Proteins
Saccharomyces cerevisiae Proteins
Temperature
Vero Cells
Vesicular Transport Proteins
Viral Envelope Proteins
English Exocytic transport from the endoplasmic reticulum (ER) to the Golgi complex has been visualized in living cells using a chimera of the temperature-sensitive glycoprotein of vesicular stomatitis virus and green fluorescent protein (ts-G-GFP[ct]). Upon shifting to permissive temperature, ts-G-GFP(ct) concentrates into COPII-positive structures close to the ER, which then build up to form an intermediate compartment or transport complex, containing ERGIC-53 and the KDEL receptor, where COPII is replaced by COPI. These structures appear heterogenous and move in a microtubule-dependent manner toward the Golgi complex. Our results suggest a sequential mode of COPII and COPI action and indicate that the transport complexes are ER-to-Golgi transport intermediates from which COPI may be involved in recycling material to the ER.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/10688
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