Journal article
Brefeldin A, a drug that blocks secretion, prevents the assembly of non-clathrin-coated buds on Golgi cisternae.
- Orci L Department of Morphology, University of Geneva Medical School, Switzerland.
- Tagaya M
- Amherdt M
- Perrelet A
- Donaldson JG
- Lippincott-Schwartz J
- Klausner RD
- Rothman JE
- 1991-03-22
Published in:
- Cell. - 1991
Animals
Biological Transport
Brefeldin A
Cell-Free System
Cells, Cultured
Cyclopentanes
Cytosol
Golgi Apparatus
English
We report that brefeldin A prevents the assembly of non-clathrin-coated vesicles from Golgi cisternae in a cell-free system. This finding provides a simple molecular explanation for the primary effect of this remarkable compound in blocking constitutive secretion. We further report that when coated vesicle assembly is blocked, extensive tubule networks form that connect previously separate cisternae and stacks into a single topological unit, allowing the intermixing of contents of Golgi cisternae, presumably by lateral diffusion. Formation of the tubule networks requires ATP, cytosol, and the general fusion protein NSF. Tubule networks may be related to the membrane tubules mediating retrograde transport in vivo.
- Language
-
- English
- Open access status
- closed
- Identifiers
-
- DOI 10.1016/0092-8674(91)90273-2
- PMID 2004424
- Persistent URL
- https://sonar.ch/global/documents/10794
Statistics
Document views: 40
File downloads: