Nucleosome positioning at the replication fork.
- Lucchini R Institute of Cell Biology, ETH Hönggerberg, CH-8093 Zürich, Switzerland.
- Wellinger RE
- Sogo JM
- 2001-12-18
Published in:
- The EMBO journal. - 2001
Base Sequence
Chromatin
DNA Primers
DNA Replication
DNA, Fungal
DNA, Ribosomal
Exonucleases
Nucleosomes
Saccharomyces cerevisiae
English
In order to determine the time required for nucleosomes assembled on the daughter strands of replication forks to assume favoured positions with respect to DNA sequence, psoralen cross-linked replication intermediates purified from preparative two-dimensional agarose gels were analysed by exonuclease digestion or primer extension. Analysis of sites of psoralen intercalation revealed that nucleosomes in the yeast Saccharomyces cerevisiae rDNA intergenic spacer are positioned shortly after passage of the replication machinery. Therefore, both the 'old' randomly segregated nucleosomes as well as the 'new' assembled histone octamers rapidly position themselves (within seconds) on the newly replicated DNA strands, suggesting that the positioning of nucleosomes is an initial step in the chromatin maturation process.
- Language
-
- English
- Open access status
- green
- Identifiers
-
- DOI 10.1093/emboj/20.24.7294
- PMID 11743005
- Persistent URL
- https://sonar.ch/global/documents/111492
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