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Encoded libraries of chemically modified peptides.
Journal article

Encoded libraries of chemically modified peptides.

  • Heinis C Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland. Electronic address: christian.heinis@epfl.ch.
  • Winter G MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, Francis Crick Avenue, Cambridge CB2 0QH, United Kingdom. Electronic address: winter@mrc-lmb.cam.ac.uk.
  • 2015-03-14
Published in:
  • Current opinion in chemical biology. - 2015
Combinatorial Chemistry Techniques
Cyclization
DNA
Drug Discovery
Gene Expression
Gene Library
Humans
Peptide Library
Peptides, Cyclic
Peptidomimetics
Small Molecule Libraries
English The use of powerful technologies for generating and screening DNA-encoded protein libraries has helped drive the development of proteins as pharmaceutical ligands. However the development of peptides as pharmaceutical ligands has been more limited. Although encoded peptide libraries are typically several orders of magnitude larger than classical chemical libraries, can be more readily screened, and can give rise to higher affinity ligands, their use as pharmaceutical ligands is limited by their intrinsic properties. Two of the intrinsic limitations include the rotational flexibility of the peptide backbone and the limited number (20) of natural amino acids. However these limitations can be overcome by use of chemical modification. For example, the libraries can be modified to introduce topological constraints such as cyclization linkers, or to introduce new chemical entities such as small molecule ligands, fluorophores and photo-switchable compounds. This article reviews the chemistry involved, the properties of the peptide ligands, and the new opportunities offered by chemical modification of DNA-encoded peptide libraries.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/112017
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